SPAD GREENNESS TO ESTIMATE GENOTYPIC VARIATION IN FLAG LEAF CHLOROPHYLL IN SPRING WHEAT UNDER MEDITERRANEAN CONDITIONS

Year 2015, , 1 - 8, 18.05.2015

Celaleddin Barutcular , İrem Toptas Hatice Turkten Mehmet Yıldırım Mujde Koc

https://doi.org/10.17557/.51440

Cited By: 3

Abstract

Leaf chlorophyll (Chl) is emphasized as an indicator for photosynthesis in wheat (Triticum aestivum L.). SPAD greenness meters are used to predict extractable Chl, but few studies have evaluated relationships between flag leaf greenness and Chl among wheat genotypes. Sixteen spring wheat genotypes with similar development patterns were studied in eight environments (2 years, 2 irrigation treatments and 2 sowing times) to investigate the precision of the SPAD-502 meter to predict Chl content/concentration. Relationships of Chl with SPAD greenness generally best fit linear and quadratic models.  Relationships of SPAD greenness with Chl concentration were weak and inconsistent. Strongest associations were observed with Chl_a content (highest R² = 0.71 under late sowing), whereas associations with Chl_b content were weak (highest R² = 0.46) or insignificant. Relationships  between  SPAD greenness and  total Chl  content ranged from low (R² = 0.24, p = 0.054) to moderate (R² = 0.64, p < 0.001) under rainfed conventional-sowing and late-sowing conditions in the second year, respectively. SPAD greenness can be used to diagnose spring wheat genotypes differing in flag leaf Chl_a and total Chl content under warm Mediterranean conditions, but may not always applicable in cooler Mediterranean conditions, where genetic variability, especially in Chl_b, was not expressed adequately.

Keywords

Chlorophyll meter, Chlorophyll content, Chlorophyll concentration, Flag leaf, Spring wheat, drought

References

• LITERATURE CITED
• Araus, J.L., G.A. Slafer, C. Royo, M.D. Serret. 2008. Breeding for Yield Potential and Stress Adaptation in Cereals. Critical Reviews in Plant Sci. 27: 377–412.
• Arnon, D.I.. 1949. Copper enzymes in isolated chloroplasts; Polyphenoloxidases in Beta vulgaris. Plant Physiol. 24: 1–15.
• Babar, M.A, M.P. Reynolds, M. Van Ginkel, A.R. Klatt, W.R. Raun, M.L. Stone. 2006. Spectral Reflectance to Estimate Genetic Variation for In-Season Biomass, Leaf Chlorophyll, and Canopy Temperature in Wheat. Crop Sci. 46: 1046–1057.
• Bindi, M., A. Hacour, K. Vandermeiren, J. Craigon, K. Ojanpera, G. Sellden, P. Hogy, J. Finnan, L. Fibbi. 2002. Chlorophyll concentration of potatoes grown under elevated carbon dioxide and/or ozone concentrations. European Journal of Agron. 17: 319 -335.
• Campbell, R.J., K.N. Mobley, R.P. Marini, D.G. Pfeiffer. 1990. Growing conditions alter the relationship between SPAD-501 values and apple leaf chlorophyll. Hortsci. 25: 330–331.
• Castelli, F., R. Contillo, F. Miceli. 1996. Non-destructive Determination of Leaf Chlorophyll Content in Four Crop Species. Journal of Agronomy and Crop Sci. 177: 275–283.
• Del Blanco, I.A., S. Rajaram, W.E. Kronstad, M.P. Reynolds. 2000. Physiological performance of synthetic hexaploid wheat-derived populations. Crop Sci. 40: 1257-1263.
• Evans, J.R.. 1983. Nitrogen and photosynthesis in the flag leaf of wheat (Triticum aestivum L.). Plant Physiol. 72: 297 - 302.
• Fritschi, F.B., J.D. Ray. 2007. Soybean leaf nitrogen, chlorophyll content, and chlorophyll a/b ratio. Photosynthetica 45 (1): 92-98.
• Gutiérrez-Rodríguez, M., M.P. Reynolds, A. Larqué-Saavedra. 2000. Photosynthesis of wheat in a warm, irrigated environment II. Traits associated with genetic gains in yield. Field Crops Research 66: 51-62.
• Hoel, B.O., K.A. Solhaug. 1998. Company Effect of Irradiance on Chlorophyll Estimation with The Minolta SPAD-502 Leaf Chlorophyll Meter. Annals of Bot. 82: 389-392.
• Jifon, J.L., J.P. Syvertsen, E. Whaley. 2005. Growth environment and leaf anatomy affect non-destructive estimates of chlorophyll and nitrogen in Citrus sp. leaves. Journal of American Society of Horticultural Sci., 130: 152 – 158.
• Lichtenthaler, H.K., A.R.Wellburn. 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11: 591–592.
• Lopes, M.S., M.P. Reynolds. 2012. Stay-green in spring wheat can be determined by spectral reflectance measurements (normalized difference vegetation index) independently from phenology. Journal of Experimental Bot. 63: 3789-3798.
• Markwell, J., J.C. Osterman, J.L. Mitchell. 1995. Calibration of the Minolta SPAD-502 leaf chlorophyll meter. Photosynthesis Res. 46: 467-472.
• Marquard, R.D., J.L. Tipton. 1987. Relationship between extractable chlorophyll and an in situ method to estimate leaf greenness. HortSci. 22: 1327
• Martínez, D.E., J.J. Guiamet. 2004. Distortion of the SPAD 502 chlorophyll meter readings by changes in irradiance and leaf water status. Agronomie, 24, 41-46.
• Monje, O.A., Bugbee B.. 1992. Inherent limitations of nondestructive chlorophyll meters: A comparison of two types of meters. HortSci. 27: 69-71.
• Neufeld, H.S., A.H. Chappelka, G.L. Somers, K.O. Burkey, A.W. Davison, P.L. Finkelstein. 2006. Visible foliar injury caused by ozone alters the relationship between SPAD meter readings and chlorophyll concentrations in cutleaf coneflower. Photosynthesis Res. 87, 281–286.
• Palta, J.P.. 1990. Leaf chlorophyll content. Remote Sensing Reviews, 5: 207-213.
• Parry, M.A.J., M. Reynolds, M.E. Salvucci, C. Raines, P.J. Andralojc, X-G. Zhu, G.D. Price, A.G. Condon, R. Furbank. 2011. Raising Yield Potential of Wheat: (II) Increasing photosynthetic capacity and efficiency. Journal of Experimental Bot. 62: 453-467.
• Porra, R.J., W.A. Thompson, P.E. Kreidemann. 1989. Determination of accurate extinctions coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta (BBA) – Bioenergetics, 975: 384–394.
• Reynolds, M.P., M. Balota, M.I.B. Delgado, I. Amani, R.A.Fischer. 1994. Physiological and morphological traits associated with spring wheat yield under hot, dry irrigated conditions. Australian Journal of Plant Physiol. 21: 717-730.
• Reynolds, M.P., M.I.B. Delgado, M. Gutierrez-Rodriguez, A. Larque-Saavedra. 2000. Photosynthesis of wheat in a warm, irrigated environment. I. Genetic diversity and crop productivity. Field Crops Res. 66: 37–50.
• Richardson, A.D., S.P. Duigan, G.P. Berlyn. 2002. An evaluation of noninvasive methods to estimate foliar chlorophyll content. New Phytologist, 153: 185-194.
• Ruiz-Espinoza, F. H., B. Murillo-Amador, J. L. García-Hernández, L. Fenech-Larios, E. O. Rueda-Puente, E. Troyo-Diéguez , C. Kaya, A. Beltrán-Morales. 2010. Field evaluation of the relationship between chlorophyll content in basil leaves and a portable chlorophyll meter (SPAD-502) readings. Journal of Plant Nutrition 33:423 - 438.
• SPSS, 1999. SYSTAT User’s Guide: Statistics. Version 9.0. SPSS, Inc., Chicago, IL
• Uddling, J., J. Gelang-Alfredsson, K. Piikki, H. Pleijel. 2007. Evaluating the relationship between leaf chlorophyll concentration and SPAD-502 chlorophyll meter readings. Photosynthesis Res. 91: 37–46.
• Xiao, Y.G., Z.G. Qian, K. Wu, J.J. Liu, X.C. Xia, W.Q. Ji, Z.H. He. 2012. Genetic gains in grain yield and physiological traits of winter wheat in Shandong Province, China, from 1969 to 2006. Crop Sci. 52: 44–56.
• Yamamoto, A., T. Nakamura, J.J. Adu-Gyamfi, M. Saigusa. 2002. relationship between chlorophyll content in leaves of sorghum and pigeonpea determined by extraction method and by chlorophyll meter (SPAD-502). Journal of Plant Nutrition 25(10): 2295 – 2301.
• Zadoks, J.C., T.T. Chang, C.F. Konzak. 1974. A decimal code for the growth stages of cereals. Weed Res. 14: 415–421.
• Zhang, C.J., G.X. Chen, X.X. Gao, C.J. Chu. 2006. Photosynthetic decline in flag leaves of two field-grown spring wheat cultivars with different senescence properties. South African Journal of Bot. 72: 15 -23.