IDENTIFICATION OF BLUE‑ALEURONE WHEAT DERIVATIVES BY SEQUENTIAL GISH-FISH ASSAY

Year 2017, , 253 - 257, 15.12.2017

Wang Weı Chen Tıanqıng Sui Jıanshu Wang Yanlı Chai Shouxı He Qıngcaı

https://doi.org/10.17557/tjfc.359699

Abstract

To select stable inheritable disomic substitution line from the filial generation of blue-grain wheat and common
wheat high effectively, sequential GISH (genomic in situ hybridization) and multicolor FISH (fluorescence in
situ hybridization) assay was used to investigate the number and characteristics of chromosome 4E in F4 and
F6 hybrid offspring. The F4 and F6 plant were selected by the blue aleurone layer future, in which the bluegrain
genes from Thinopyrum species were existed. GISH assay showed that the rate of stable inheritable
disomic substitution line (blue-grain trait in common wheat) was above 80 percent in F6 plants, but lower in F4
plants. Specially, all of the F6 progenies of ‘qian 09206’, which was derived from crossing parents ‘0147’ and
common wheat ’9938’, were homozygous disomic substitution lines. The sequential GISH-FISH assay showed
that F4 progeny (B-228) from filial generation of ‘Neimai 10’ and ‘0147’ was a 4D/4E stable inheritable alien
disomic substitution line. These results showed that the color based selection was not effective. However,
combining color based selection with sequential GISH-FISH assay can improve wheat breeding efficiency. 

Keywords

Blue-grained wheat, chromosome 4E, FISH, GISH

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