IDENTIFICATION OF A SCAR MARKER LINKED TO A SHATTERING RESISTANCE TRAIT IN SESAME

Abstract

Sesame (Sesamum indicum L.) is one of the most important oil crops in temperate and tropical regions and is grown worldwide over an area of 5179 (hg ha-1 ) to produce 5.469.024 tonnes of seed. Capsule shattering before or during harvest can cause yield losses of greater than 50%. The objectives of the present study were to evaluate the inheritance of resistance to capsule shattering in the F2 derived from a cross between Cplus1, a sesame line with shattering-resistant capsules (Sh1Sh1Sh2Sh2), and KUAOX25, a line with shatteringsusceptible capsules (sh1sh1sh2sh2- ); and use bulked segregant analysis and Amplified Fragment Length Polymorphisms (AFLPs) to identify Sequence Characterized Amplified Region (SCAR) markers associated with shattering resistance. After screening 192 AFLP primer combinations, nine polymorphic bands were identified, and one of these AFLPs was developed into a Si-SR-32-19 SCAR that could distinguish between shattering-susceptible and shattering-resistant phenotypes.

Keywords

• AFLP,BSA,SCAR,Sesamum indicum L.,shattering resistance

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