IDENTIFICATION OF A SCAR MARKER LINKED TO A SHATTERING RESISTANCE TRAIT IN SESAME

Year 2017, , 258 - 265, 15.12.2017

Chalermpol Phumıchaı Weerachai Matthayatthaworn Nipha Chuenpom Arunee Wongkaew Phakaked Somsaeng Tanapong Yodyıngyong Pherawich Panklang Sujin Jenweerawat Yaowamarn Keawsaard Thitaporn Phumıchaı Tanee Sreewongchaı Rangsarid Kaveeta

https://doi.org/10.17557/tjfc.359707

Cited By: 2

Abstract

Sesame (Sesamum indicum L.) is one of the most important oil crops in temperate and tropical regions and is
grown worldwide over an area of 5179 (hg ha-1
) to produce 5.469.024 tonnes of seed. Capsule shattering before
or during harvest can cause yield losses of greater than 50%. The objectives of the present study were to
evaluate the inheritance of resistance to capsule shattering in the F2 derived from a cross between Cplus1, a
sesame line with shattering-resistant capsules (Sh1Sh1Sh2Sh2), and KUAOX25, a line with shatteringsusceptible
capsules (sh1sh1sh2sh2-
); and use bulked segregant analysis and Amplified Fragment Length
Polymorphisms (AFLPs) to identify Sequence Characterized Amplified Region (SCAR) markers associated
with shattering resistance. After screening 192 AFLP primer combinations, nine polymorphic bands were
identified, and one of these AFLPs was developed into a Si-SR-32-19 SCAR that could distinguish between
shattering-susceptible and shattering-resistant phenotypes.

Keywords

AFLP, BSA, SCAR, Sesamum indicum L., shattering resistance

References

• Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSIBLAST: A new generation of protein database search programs. Nucleic Acids Res. 25: 3389–3402.
• Anilakumar, K.R., A. Pal, F. Khanum, and A.S. Bawa. 2010. Nutritional, medicinal and industrial uses of sesame (Sesamum indicum L.) seeds: An overview. Agric. Conspec. Sci. 75:159–168
• Arslan, C., B. Uzun, S. Ulger and M.I. Cagirgan. 2007. Determination of oil content and fatty acid composition of sesame mutants suited for intensive management conditions. J Am Oil Chem Soc. 84(10): 917-920.
• Ashri, A. 1998. Sesame breeding. Plant Breed. Rev. 16:179–228. Ashri, A. 2010. Sesame breeding. In: Plant Breed Rev., ed. Janick, J., Volume 16, Wiley, Oxford.
• Ashutosh, A., P.C. Sharma, S. Prakash and S.R. Bhat. 2007. Identification of AFLP markers linked to the male sterility restorer gene of CMS (Moricandia arvensis) Brassica juncea and conversion to SCAR markers. Theor. Appl. Genet. 114: 385–392.
• Benbouza, H., J.M. Jacquemin, J.P. Baudoin and G. Mergeai. 2006. Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels. Biotechnol. Agron. Soc. Environ. 10: 77–81.
• Cagirgan, M.I. 1996. Radiosensitivity of Turkish sesame to gamma-rays. Turk.J. Field Crops 1: 39–43.
• Cagirgan, M.I. 2001. Mutation techniques in sesame (Sesamum indicum L.) for intensive management: confirmed mutants. In: Sesame Improvement by Induced Mutations. Final report of an FAO/IAEA co-ordinated research project organized by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture. 31–40. International Atomic Energy Agency (IAEA).
• Cagirgan, M.I. 2006. Selection and morphological characterization of determinate mutants in sesame. Field Crops Res. 96:19–24.
• Cagirgan, M.I., S. Ozerden and M.O. Ozbas. 2009. Agronomic trait assessment and selection for number of capsules in determinate x indeterminate crosses of sesame. Turk J Agric For. 33: 231–241.
• Cosio, C. and C. Dunand. 2010. Transcriptome analysis of various flower and silique development stages indicates a set of class III peroxidase genes potentially involved in pod shattering in Arabidopsis thaliana. BMC Genomics 11: 528.
• Dussle, C.M., M. Quint, A.E. Melchinger, M.L. Xu and T. Lübberstedt. 2003. Saturation of two chromosome regions conferring resistance to SCMV with SSR and AFLP markers by targeted BSA. Theor. Appl. Genet. 106: 485–493.
• FAOSTAT. 2014. FAOSTATdatabase. FAO,Rome,Italy. http://faostat3.fao.org/browse/Q/QC/E, (Accessed July 25, 2016)
• Ferrandiz, C., S.J. Liljegren and M.F. Yanofsky.2000. Negative regulation of the SHATTERPROOF genes by FRUITFULL during Arabidopsis fruit development Science 289: 436–438.
• Girin, T., P. Stephenson, C.M.P. Goldsack, S.A. Kempin, A. Perez, N. Pires, P.A. Sparrow, T.A. Wood, M.F. Yanofsky and L. Østergaard. 2010. Brassicaceae INDEHISCENT genes specify valve margin cell fate and repress replum formation. Plant J. 63: 329–338.
• Hayashi, M., A. Ujiie, H. Serizawa, H. Sassa, H. Kakui, T. Oda and T. Koba. 2011. Development of SCAR and CAPS markers linked to a recessive male sterility gene in lettuce (Lactuca sativa L.). Euphytica 180: 429–436.
• Jenkins, E.S., W. Paul, M. Craze, C.A. Whitelaw, A. Weigand and J.A. Roberts. 1999. Dehiscence-related expression of an Arabidopsis thaliana gene encoding a polygalacturonase in transgenic plants of Brassica napus. Plant Cell Environ. 22: 159–167.
• Ke, L.P., Y.Q. Sun, P.W. Liu and G.S. Yang. 2004. Identification of AFLP fragments linked to one recessive genic male sterility (RGMS) in rapeseed (Brassica napus L.) and conversion to SCAR markers for marker-aided selection. Euphytica 138: 163–168.
• Ke, T., C. Dong, H. Mao, Y. Zhao, H. Chen, H. Liu, X. Dong, C. Tong and S. Liu. 2011. Analysis of expression sequence tags from a full-length-enriched cDNA library of developing sesame seeds (Sesamum indicum). BMC Plant Biol. 11: 180.
• Kotcha, A., W. Wongyai, P. Pongtongkam and J. Verawudh. 2012. Inheritance of shatter resistance capsules in sesame. Thai J. Genet. 5:149–158 (in Thai).
• Langham, D.G. 1946. Genetics of sesame III: “open sesame” and mottled leaf. J. Hered. 37: 149–152.
• Langham, D.G. and M. Rodriguez. 1946. Abrete 265esame: Ajonjoli (Sesamum indicum) que no pierde semillas. Circ. 17, Publ. Ministerio de Agricultura y Cria, Maracay, Venezuela.
• Langham, D.G., M. Rodriguez and E. Reveron. 1956. Dehiscencia, y otras caracteristicas del ajonjoli Sesamum indicum L., en relacion con el problema de la cosecha. Genesa, Publ. Tecnica 1, Maracay, Venezuela.
• Langham, D.R. 2001. Shatter resistance i192 AFLP primer combination n sesame. p. 51–61. In: L. Van Zanten (ed.), Sesame improvements by induced mutations, Proc. Final FAO/IAEA Co-ord. Res. Mtng, IAEA, Vienna, TECDOC- 1195.
• Langham, D. R. and T. Wiemers. 2002. Progress in mechanizing sesame in the US through breeding. In: Trends in New Crops and New Uses, eds. Janick, J. and Whipkey, A., 157–173, ASHS Press, Alexandria.
• Lenser, T. and G. Theißen. 2013. Conservation of fruit dehiscence pathways between Lepidium campestre and Arabidopsis thaliana sheds light on the regulation of INDEHISCENT. Plant J. 76:545–556.
• Liljegren, S.J., A.H. Roeder, S.A. Kempin, K. Gremski, L. Østergaard, S. Guimil, D.K. Reyes and M.F. Yanofsky. 2004. Control of fruit patterning in Arabidopsis by INDEHISCENT. Cell 19:843–853.
• Liljegren, S.J., G.S. Ditta, Y. Eshed, B. Savidge, J.L. Bowman and M.F. Yanofsky. 2000. SHATTERPROOF MADS-box genes control seed dispersal in Arabidopsis. Nature 404:766– 770.
• Michelmore, R.W., I. Paran and R.V. Kesseli. 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. 88: 9828–9832.
• Microsoft. 2010. Excel. Microsoft Office 2010. Microsoft, Redmond, WA.
• Mitsuda, N., A. Iwase, H. Yamamoto, M. Yoshida, M. Seki, K. Shinozaki and M. Ohme-takagi. 2007. NAC transcription factors, NST1 and NST3, are key regulators of the formation of secondary walls in woody tissues of Arabidopsis. Plant Cell. 19: 270–280.
• Negi, M.S., M. Devic, M. Delseny and M. Lakshmikumaran. 2000. Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection. Theor. Appl. Genet. 101:146–152. Ogawa, M., P. Kay, S. Wilson and S.M. Swain. 2009.
• Arabidopsis dehiscence zone polygalacturonase1 (ADPG1), ADPG2, and QUARTET2 are polygalacturonases required for cell separation during reproductive development in Arabidopsis. Plant Cell. 21:216–233.
• Østergaard, L., S.A. Kempin, D. Bies, H.J. Klee and M.F. Yanofsky. 2006. Pod shatter resistant Brassica fruit produced by ectopic expression of the FRUITFULL gene. Plant Biotech. J. 4:45–51.
• Rajani, S. and V. Sundaresan. 2001. The Arabidopsis myc/bHLH gene ALCATRAZ enables cell separation in fruit dehiscence. Curr. Biol. 11:1914–1922.
• Rozen, S. and H. Skaletsky. 2000. Primer3 on the www for general users and for biologist programmers. Methods of Molecular Biology 132: 365–386.
• Silme, R.S. and M.I. Cagirgan. 2010. Screening for resifigstance to fusarium wilt in induced mutants and world collection of sesame under intensive management. Turkish Journal of Field Crops 15:89–93.
• Tan, X., Z. Xia, L. Zhang, Z. Zhang, Z. Guo and C. Qi. 2009. Cloning and sequence analysis of oilseed rape (Brassica napus) SHP2 gene. Bot. Stud. 50:403–412.
• Tashiro, T., Y. Fukuda, T. Osawa and M. Namiki. 1990. Oil and minor components of sesame (Sesamum indicum L.) strains. J. Am. Oil Chem. Soc. 67:508–511.
• Vos, P., R. Hogers, M. Bleeker, M. Reijans, T.V. de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M. Zabeau. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23:4407–4414.
• Wongyai, W. and S. Chowchong. 2003. C plus 1: the new white sesame variety with non-shattering. Proceedings of the 3rd National Conference on Sesame, Sunflower, Castor and Safflower, Chiang Mai, Thailand (in Thai).