GENETIC VARIATION AMONG EGYPTIAN WHITE LUPIN (LUPINUS ALBUS L.) GENOTYPES

Abstract

Field Evaluation of eighteen lupin genotypes was carried out during 2012/13 and 2013/14 at Giza Agriculture Research Station, Egypt, to assess the genetic variation on phenological parameters. Simultaneously, molecular diversity assessment was performed using 11 SRAP primer pair combinations. High significant differences among genotypes were observed for field performance during the two seasons and their combined data. The Australian genotype 75 B9.10 and landrace Fayed1 recorded the highest seed yield/plant while the Egyptian landraces Sohag2, Fayed1 and the cultivar Giza1 were superiors in seed yield/hectare (2.8, 2.6 and 2.6t, respectively). Only the first two principle components explained 97 % of variability. Characters, number of pods, seed yield/plant and seed yield/hectare were grouped on the positive PC1 axis of the biplot with genotypes Sohag2, 75B9.10 and Fayed1. The molecular analysis revealed coherent results. The 11 SRAP primers generated 3286 amplified fragments represents 337 genetic loci across the lupin genome. The average detected loci per primer pair was 30.64 with 322 polymorphic loci across the studied genotypes (96.05%). The polymorphism information content (PIC) values were generally high and ranged from 0.883 to 0.981. The high PIC values highlight the power of SRAP markers in detecting the molecular diversity in lupin genotypes. The genotypes tended to cluster based on their origin and genetic background.

Keywords

• biplot,cluster analysis,lupin,marker,SRAP

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