SEED COAT THICKNESS DIFFERENTIATION AND GENETIC POLYMORPHISM FOR Lupinus mutabilis SWEET BREEDING

Abstract

The paper investigates seed coat characteristics in L. mutabilis, a potential forage crop. In the study twelve genotypes, including three epigonal lines KW-5, KW-10, Pop.1 and lines with traditional type of growth XM.5, Pop.15 LM.13, LM.34, ‘Potosi’, Mut-45, Mut-160, Mut-136, Mut-628 were evaluated. The highest seed coat percentage was recorded in mutant line Mut-160 and the lowest was noted for mutant line Mut-45. The statistical significance relationship between seed coat thickness and 1000 seeds weight was not notice. It’s gives capabilities for selection of favorable genotypes in breeding process. In order to illustrate genetic diversity among the genotypes tested, 24 ISSR primers and 6 pairs of AFLP primers were used. They generated a total of 685 polymorphic amplification products in 12 evaluated Andean lupin genotypes. The products characteristic only for the finest seed coat lines Mut-45 were detected as well as for the thickness seed coat line Mut-160.

Keywords

• AFLP markers,genetic diversity,ISSR markers,Lupinus mutabilis,seed coat proportion,seed coat ultrastructure

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