EFFECTS OF ORGANIC, CHEMICAL AND INTEGRATED NUTRITION SYSTEMS ON MORPHO-PHYSIOLOGICAL TRAITS OF OREGANO (Origanum vulgare L.)

Abstract

In order to evaluate the effects of organic, chemical and integrated nutrition systems on morpho-physiological

traits of oregano (Origanum vulgare L.), an experiment was carried out at the experimental farm of the

Agricultural and Natural Resources Research Center, Urmia, Iran, during 2016-2017 growing season. The

experimental design was a completely randomized block design with three replications. The experimental

treatments included NPK, two organic (vermicompost and biochar), two biological (nitroxin and bio super

phosphate) fertilizers and their all twin combinations and control (non-fertilized). Our results showed that the

application of organic fertilizers caused a significant increase in yield and essential oil content in oregano. On

the other hand, integrated nutrition treatments had the most effect on increasing the traits in compared with

the individual treatments. The highest essential oil yield was obtained from Nitroxin treatment (871.27 kg ha-1)

in the second year. Whereas, the lowest value of essential oil yield was found in control (non-fertilized) (25.92

kg ha-1) in the first year. The present study clearly indicated that chemical fertilizers could be replaced by biofertilizers

resulted in similar oil yield.

Keywords

• Biochar,biological fertilizers,essential oil yield

References

1. Al-Fraihat, A.H., S.Y.A. Al-dalain, Z.B. Al-Rawashdeh, M.S. Abu-Darwish and J.A. Al-Tabbal. 2011. Effect of organic and biofertilizers on growth, herb yield and volatile oil of Marjoram plant grown in Ajloun region, Jordan. Medicinal Plants Research 5(13): 2822-2833.
2. Aligianis, N., E. Kalpoutzakis, S. Mitaku and I.B. Chinou. 2001. Composition and antimicrobial activity of the essential oil from Origanum species. Agricultural and Food Chemistry 49(9): 4168-4170.
3. Anjum, M., M. Sajjad, N. Akhtar, M. Quereshi, A. Iqbal, A. Jami and M. Hassan. 2007. Response of Cotton to plant growth promoting rhizobacteria (PGPR) inoculation under different levels of nitrogen. Agricultural Research 45: 135- 143.
4. Anwar, M., D.D. Patra, S. Chand, A. Kumar, A.A. Naqvi and S.P.S. Khanuja. 2005. Effect of organic manure and inorganic fertilizers on growth, herb, oil yield, nutrient accumulation and oil quality of French basil (Ocimum basilcum L.). Communications in Soil Science and Plant Analysis. 36: 1737-1746.
5. Arancon, N.Q., C.A. Edwards, P. Bierman, C. Welch and J.D. Metzger. 2004. Influences of vermicompost on field Strawberries: 1. Effects on growth and yields. Bioresource Technology 93: 145-153.
6. Atkinson, C.J., J.D. Fitzgerald and N.A. Hipps. 2010. Potential mechanisms for achieving agricultural benefits from biochar application to temperate soils: a review. Plant and Soil. 337(1): 1-18.
7. Azzaz, N.A., E.A. Hassan and E.H. Hamad. 2009. The chemical constituent and vegetative and yielding characteristics of Fennel plants treated with organic and biofertilizer instead of mineral fertilizer. Australian Journal of Basic and Applied Sciences 3(2): 579-587.
8. Bajeli, J., S. Tripathi, A. Kumar and R.K. Upadhyay. 2016. Organic manures a convincing source for quality production of Japanese mint (Mentha arvensis L.). Industrial Crops and Products. 83: 603-606.

9. Banchio, E., P.C. Bogino, J. Zygadlo and W. Giordano. 2008. Plant growth promoting rhizobacteria improve growth and essential oil yield in (Origanum majorana L.). Biochemical Systematics and Ecology. 36(10): 766-771.
10. Clevenger, J.H. 1928. Apparatus for the determination of volatile oil. American Pharmaceutical Association. 17(4): 346-349.
11. Copetta, A., G. Lingua and G. Berta. 2006. Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil production in Ocimum basilicum L. var. Genovese. Mycorrhiza. 16: 485-494.
12. Darzi, M.T., M.R. Haj-Seyed-Hadi and F. Rejali. 2012. Effects of the application of vermicompost and nitrogen fixing bacteria on quantity and quality of the essential oil in Dill (Anethum graveolens). Medicinal Plants Research 6(21): 3793-3799.
13. Ding Y., Y.X. Liu, W.X. Wu, D.Z. Shi, M. Yang and Z.K. Zhong. 2010. Evaluation of biochar effects on nitrogen retention and leaching in multi-layered soil columns. Water, Air, and Soil Pollution. 213(1): 47-55.
14. Fatma, E.M., I. El-Zamik, T. Tomader, H.I. El-Hadidy, L. Abd El-Fattah and H. Seham-Salem. 2006. Efficiency of biofertilizers, organic and in organic amendments application on growth and essential oil of Marjoram (Majorana hortensis L.) plants grown in sandy and calcareous. Agric. Microbiology Dept., Zagazig University and Soil Fertility and Microbiology Department. Desert Research Center. Cairo. Egypt, p. 212-264.
15. Gharib, F.A., L.A. Moussa and O.N. Massoud. 2008. Effect of compost and Bio-fertilizers on growth, yield and essential oil of sweet Marjoram (Majorana hortensis) plant. International Journal of Agriculture & Biology 10(4): 381-387.
16. Gravel, V., H. Antoun and R. Tweddell. 2007. Growth stimulation and fruit yield of greenhouse tomato plants by inoculation with Pseudomonas putida or Trichoderma atroviride: Possible role of indole acetic acid (IAA). Soil Biology and Biochemistry 39: 1968-1977.
17. Grichko, V.P. and B.R. Glick. 2001. Amelioration of flooding stress by ACC deaminase-containing plant growthpromoting bacteria. Plant Physiology and Biochemistry. 39: 11-17.
18. GUENTHER, E. 1961. The essential oils. D. von Nostrand Company Press. NY.
19. Hassan, F.A.S. and E.F. Ali. 2014. Impact of different water regimes based on class-A pan on growth, yield and oil content of Coriandrum sativum L. plant. Journal of the Saudi Society of Agricultural Sciences 13(2): 155-161.
20. Kapoor, R., B. GIRI and K.G. Mukerji. 2004. Improved growth and essential oil yield and quality in Foeniculum vulgare Mill. on mycorrhizal inoculation supplemented with Pfertilizer. Bioresource Technology 93(3): 307-311.
21. Karla, A. 2003. Organic cultivation of medicinal and aromatic plants. A hope for sustainability and quality enhancement. Journal of Organic production of Medicinal, Aromatic and Dye-Yielding Plants (MADPs), FAO. 198 p.
22. Karthikeyan, B., C. Abdul Jaleel, G.M.A. Lakshmanan and M. Deiveekasundaram. 2008. Studies on rhizosphere microbial diversity of some commercially important medicinal plants. Colloids and Surfaces B: Biointerfaces. 62(1): 143-145.
23. Khalid, K.A. and M.S. Hussein. 2012. Effect of cattle and liquid manures on essential oil and antioxidant activities of celery (Apium graveolens L.) Fruits. Essential Oil Bearing Plants. 15: 97-107.
24. Kim, K.R., J.G. Kim, J.S. Park, M.S. Kim, G. Owens, G.H. Youn and J.S. Lee. 2012. Immobilizer-assisted management of metal-contaminated agricultural soils for safer food production. Environmental management 102: 88-95.
25. Laboski, C.A.M. and J.A. Lamb. 2003. Changes in soil test phosphorus concentration after application of manure or fertilizer. Soil Science Society of America Journal. 67(2): 544-554.
26. Lehmann, J. 2007. Bio-energy in the black. Frontiers in Ecology and the Environment 5: 381-387.
27. LEU, A. 2007. Organics and soil carbon: Increasing soil carbon, crop productivity and farm profitability. In Managing the Carbon Cycle, Katanning, Western Australia, Workshop, p. 19-26.
28. Manna, M.C., P.K. Ghosh, B.N. Ghosh and K.N. Singh. 2001. Comparative effectiveness of phosphate-enriched compost and single superphosphate on yield, uptake of nutrients and soil quality under soybean-wheat rotation. Agricultural Sciences 137: 45-54.
29. Marinari, S., G. Masciandaro, B. Ceccanti and S. Grego. 2000. Influence of organic and mineral fertilizers on soil biological and physical properties. Bioresource Technology 72(1): 9- 17.
30. Maurya, B.R., V.S. Meena and O.P. Meena. 2014. Influence of Inceptisol and Alfisol’s potassium solubilizing bacteria (KSB) isolates on release of K from Waste mica. Vegetos. 27(1): 181-187.
31. Mehnaz, S. and G. Lazarovits. 2006. Inoculation effects of Pseudomonas putida, Gluconacetobacter azotocaptans and Azospirillum lipoferum on Corn plant growth under greenhouse conditions. Microbial Ecology 51(3): 326-335.
32. Melero, S., E. Madejon, J.F. Herencia and J.C. Ruiz. 2007. Biochemical properties of two different textured soils (loam and clay) after the addition of two different composts during conversion to organic farming. Spanish Journal of Agricultural Research 5(4): 593-604.
33. Meena, O.P., B.R. Maurya and V.S. Meena. 2013. Influence of K-solubilizing bacteria on release of potassium from waste mica. Agriculture for Sustainable Development. 1(1): 53-56.
34. Meena, V.S., B.R. Maurya and I. Bahadur. 2014. Potassium solubilization by bacterial strain in Waste mica. Bangladesh Journal of Botany 43(2): 235-237.
35. Moradi, R., P. Rezvani-Moghaddam, M. Nasiri-Mahallati and A. Nezhadali. 2011. Effects of organic and biological fertilizers on fruit yield and essential oil of sweet fennel (Foeniculum vulgare var. dulce). Spanish Journal of Agricultural Research 9(2): 546-553.
36. Nejatzadeh-Barandozi, F. and B. Pourmaleknejad. 2014. Effect of Biological, Organic and Chemical Fertilizers on Vegetative Growth, Physiological Characteristics and Essential Oils of (Thymus vulgaris L.). Annual Research & Review in Biology 4(11): 1847-1853.
37. Ordookhani, K., K. Khavazi, A. Moezzi and F. Rejali. 2010. Influence of PGPR and AMF on antioxidant activity, lycopene and potassium contents in tomato. African Journal of Agricultural Research 5(10): 1108-1116.
38. Olsen, S.R. and J.F. Sommers. 1982. Phosphorus. In: A.L. Page (ed.), Methods of soil Analysis. Agron. No. 9, part 2: Chemical and microbiological properties, 2nd ed. American Society and Agronomy Madison, WI, USA, p. 403-430.
39. Patra, D.D., M. Anwarm and S. Chand. 2000. Integrated nutrient management and waste recycling for restoring soil fertility and productivity in Japanes mint (Mentha arvensis) and Mustard (Brassica juncea) sequence in Uttar Pradesh India. Agriculture Ecosystem and Environment 80(3): 267-275.
40. Radušienė, J., L. Ivanauskas, V. Janulis and V. Jakštas. 2008. Composition and variability of phenolic compounds in Origanum vulgare from Lithuania. Journal of Biologija. 54(1): 45-49.
41. RAO, B.R. 2001. Biomass and essential oil yields of rainfed palmarosa (Cymbopogon martinii) (Roxb.) Wats. var. motia Burk. supplied with different levels of organic manure and fertilizer nitrogen in semi-arid tropical climate. Industrial Crops and Products. 14(3): 171-178.
42. Raut, J.S. and S.M. Karuppayil. 2014. A status review on the medicinal properties of essential oils. Industrial Crops and Products. 62: 250-264.
43. Rebey, I.B., I. Jabri-Karoui, I. Hamrouni-Sellami, S. Bourgou, F. Limam and B. Marzouk. 2012. Effect of drought on the biochemical composition and antioxidant activities of cumin (Cuminum cyminum L.) seeds. Industrial Crops and Products. 36: 238-245.
44. Richter, J., M. Stutzer and I. Schellenberg. 2005. Effects of mycorrhization on the essential oil content and composition of aroma components of Marjoram (Marjorana hortensis), Thyme (Thymus vulgaris L.) and Caraway (Carum carvi L.). 36th International Symposium on Essential Oils, 4-7 September 2005; Budapest, Hungary. Roshanpour, N., M.T. Darzi and M. Haj-seyed-hadi. 2014. Effects of plant growth promoter bacteria on biomass and yield of Basil (Ocimum basilicum L.). International Journal of Advanced Biological and Biomedical Research 2(6): 2077-2085.
45. Schroth, G. and F.L. Sinclair. 2003. Trees, crops, and soil fertility: concepts and research methods. CABI Publishing, Wallingford, 437 p.
46. Seilsepour, M., E. Baniani and M. Kianirad. 2002. Effect of phosphate solubilizing microorganism (PSM) in reducing the rate of phosphate fertilizers application to Cotton crop. Proc 15th Int Meeting on Microbial Phosphate Solubilization, Salamanca University, 16-19 July 2002. Salamanca, Spain.
47. Sinha, J., C.K. Biswas, A. Ghosh and A. Saha. 2010. Efficacy of vermicompost against fertilizers on Cicer and Pisum and on population diversity of N2 fixing bacteria. Environmental Biology 31(3): 287-292.
48. Singh, R., S. Divya, A. Ashutosh and A. Karla. 2012. Technology for efficient and successful delivery of vermicompost colonized bioionculants in Pogostemon cabin (patchouli) Benth. World Journal of Microbiology and Biotechnology 28(1): 323-333.
49. Sturz, A.V. and B.R. Christie. 2003. Beneficial microbial allelopathies in the root zone. The management of soil quality and plant disease with rhizobacteria. Soil & Tillage Research. 72(2): 107-123.
50. Tilak, K.V.B.R., N. Ranganayaki, K.K. Pal, R. De, A.K. Saxena, C. Shekhar-Nautiyal, S. Mittal, A.K. Tripathi and B.N. Johri. 2005. Diversity of plant growth and soil health supporting bacteria. Current Science 89(1): 136-150.
51. Valadabadi, S.A. and H.A. Farahani. 2011. Investigation of biofertilizers influence on quantity and quality characteristics in Nigella sativa L. Horticulture and Forestry. 3(3): 88-92.
52. Vessey, J.K. 2003. Plant growth promoting Rhizobacteria as biofertilizer. Plant and Soil 255: 571-586.
53. Villegas, J. and J.A. Fortin. 2002. Phosphorous solubilization and pH changes as a result of interactions between soil bacteria and arbuscular mycorrhizal fungi on a medium containing NO3- as nitrogen source. Canadian Journal of Botany 80: 571-576.
54. Vittal-Navi, H., B.S. Shivakumar, C.K. Suresh and N. Earanna. 2006. Effect of Glomus fasciculatum and plant growth promoting rhizobacteria on growth and yield of Ocimum basilicum. Karnataka Journal of Agricultural Sciences 19(1): 17-20.
55. Yadegari, M., G.H.N. Farahani and Z. Mosadeghzad. 2012. Biofertilizers effects on quantitative and qualitative yield of Thyme (Thymus vulgaris). African Journal of Agricultural Research 7(34): 4716-4723.
56. Youssef, A.A., A.E. Edris and A.M. Gomaa. 2004. A comparative study between some plant growth regulators and certain growth hormones producing microorganisms on growth and essential oil composition of Salvia officinalis L. Plant Annual. Agricultural Science 49: 299-311.
57. Zaidi, A., M.S. Khan and M. Amil. 2003. Interactive effect of rhizotrophic microorganisms on yield and nutrient uptake of chickpea (Cicer arietinum L.). European Journal of Agronomy 19(1): 15-21.
58. Zaller, J.G. 2007. Vermicompost as a substitute for peat in potting media: Effects on germination, biomass allocation, yields and fruit quality of three tomato varieties. Scientia Horticulturae 112(2): 191-199.