FOLIAR APPLICATION OF SELENIUM, ZINC AND COPPER IN ALFALFA (Medicago sativa L.) BIOFORTIFICATION

Abstract

Biofortification of forage crops has an important role in improving the quality of plants used for animal nutrition. The field experiments were conducted in three consecutive years in Subotica, Serbia, in order to investigate the effect of Se, Zn, and Cu foliar fertilization on the yield, Se and Zn contents and nutrient efficiency, as well as on other mineral compositions of alfalfa hay. The treatments were as follows: i) control without fertilization, ii) 5 g Se ha-1, iii) 10 g Se ha-1, iv) 0.5 kg Zn ha-1, v) 1 kg Zn ha-1, vi) the combination of these two elements (0.5 kg Zn ha-1 and Se 10 g ha-1) and vii) 2% Cu solution. The application of Se, Zn, and Cu had no effect on dry yield or on crude protein, P, K, Ca, Mg, Fe, Mn, Mo, and Co contents in alfalfa hay. However, Se, Zn, and Cu fertilization significantly increased the contents of Se, Zn, and Cu in alfalfa hay. The results showed that Se and Zn contents in plant biomass were significantly correlated with the applied doses of Se (r=0.99) and Zn (r=0.99). The production years of alfalfa and the weather conditions proved to be significant factors in fertilization efficiency.

Keywords

• Animal feed,fertilization,fertilization efficiency,microelements

Supporting Institution

the HERD project

References

1. Ademi, A., E. Govasmark, A. Bernhoft, H. Bytyqi, M. Djikic, M. Manojlović, Z. Loncaric, M. Drinic, A. Filipovic and B.R. Singh. 2015. Status of selenium in sheep and dairy cow blood in Western Balkan countries. Acta Agric. Scand. A Anim. Sci. 65:9–16. Alloway, B.J. 2009. Soil factors associated with zinc deficiency in crops and humans. Environ Geochem Health. 31:537–548.
2. Ao, T., J.L. Pierce, R. Power, A.J. Pescatore, A.H. Cantor, K.A. Dawson and M.J. Ford. 2009. Effects of feeding different forms of zinc and copper on the performance and tissue mineral content of chicks. Poult. Sci. 88:2171–2175.
3. Ceylan, S., H. Soya, B. Budak, H. Akdemir and B.C. Esetlili. 2009. Effect of zinc on yield and some related traits of alfalfa. Turk J Field Crops. 14:136–143. Ćupina, B., S. Vujić, D. Krstić, B. Djurić, S. Aliu, M. Manojlović, R. Čabilovski and P. Lombnaes. 2016. Performance of legume–grass mixtures in the West Balkan region. Acta Agric. Scand. Sect. B Soil Plant Sci. 7:1–11.
4. Egner, H., H. Riehm and W.R. Domingo. 1960. Investigations of the chemical soil analysis as a basis for the evaluation of nutrient status in soil. II. Chemical extraction methods for phosphorus and potassium determination. K. Lantbruks. Hogsk. Ann. 26: 195-215 (DE)
5. Fang, Y., L. Wang, Z. Xin, L. Zhao, X. An and Q. Hu. 2008. Effect of foliar application of zinc, selenium, and iron fertilizers on nutrients concentration and yield of rice grain in China. J. Agric. Food Chem. 56:2079–2084. Fisher, G.E. 2008. Micronutrients and animal nutrition and the link between the application of micronutrients to crops and animal health. Turk J Agric For. 32:221–233.
6. Fixen, P., F. Brentrup, T. Bruulsema, F. Garcia, R. Norton and S. Zingore. 2015. Nutrient/fertilizer use efficiency: measurement, current situation and trends. In: Managing water and fertilizer for sustainable agricultural intensification, ed. Drechsel P., Heffer P., Magen H., Mikkelsen R. and Wichelns. D. 8–37, International Fertilizer Industry Association (IFA), Paris, France. Grewal, H.S. 2001. Zinc influences nodulation, disease severity, leaf drop and herbage yield of alfalfa cultivars. Plant Soil. 234:47–59. Grewal, H.S. 2010. Fertiliser management for higher productivity of established lucerne pasture. N. Z. J. Agric. Res. 53:303–314. Gupta, U.C. and S.C. Gupta. 2002. Quality of animal and human life as affected by selenium management of soils and crops. Commun. Soil Sci. Plant Anal. 33:2537–2555. Gupta, U.C., W.U. Kening and S. Liang. 2008. Micronutrients in soils, crops, and livestock. Earth Science Frontiers. 15:110–125. Hall, J.A., G. Bobe, J.K. Hunter, W.R. Vorachek, W.C. Stewart, J.A. Vanegas, TC. Estill, D. Wayne, W.D. Mosher and G.J. Pirelli. 2013. Effect of feeding selenium-fertilized alfalfa hay on performance of weaned beef calves. PLoS One 8: e58188. doi: 10.1371/journal.pone.0058188 ISO International Organization for Standardization. 2001. ISO14870:2001, Soil quality - Extraction of trace elements by buffered DTPA solution ISO International Organization for Standardization. 2005. ISO 10693:2005, Soil quality Determination of carbonate content Volumetric method ISO International Organization for Standardization. 2005. ISO 14235:2005, Soil quality Determination of organic carbon by sulfochromic oxidation Kalra, Y. 1997. Handbook of Reference Methods for Plant Analysis. CRC Press Boca Raton (USA) Lindsay, W.L. and W.A. Norvell. 1978. Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Sci Soc Am J. 42: 421-428. Manojlović, M. and B.R. Singh. 2012. Trace elements in soils and food chains of the Balkan region. Acta Agric. Scand. Sect. B Soil Plant Sci. 62:673–695. Manojlović, M. and Z. Lončarić. 2017. Selenium deficiency in regional soils affecting animal and human health in Balkan and other European countries. In: The Nexus of Soils, Plant, Animals and Human Health, ed. Singh, B.R., McLaughlin, M.J. and Brevik, E., 87-98, Schweizerbart, Stuttgart. Marijanušić, K., M. Manojlović, D. Bogdanović, R. Čabilovski and P. Lombnaes. 2017. Mineral composition of forage crops in respect to dairy cow nutrition, Bulg J. Agric. Sci. 23:204–212. Marschner, H. 1995. Mineral nutrition of higher plants. 2. ed. Academic press. Nawaz, F., M. Naeem, M.Y. Ashraf, M.N. Tahir, B. Zulfiqar, M. Salahuddin and M. Aslam. 2016. Selenium supplementation affects physiological and biochemical processes to improve fodder yield and quality of maize (Zea mays L.) under water deficit conditions. Front Plant Sci. 7:1438. 90 Nawaz, F., M.Y. Ashraf, R. Ahmad, E.A. Waraich, R.N. Shabbir and M.A. Bukhari. 2015. Supplemental selenium improves wheat grain yield and quality through alterations in biochemical processes under normal and water deficit conditions. Food Chem. 175:350–357. Nikolic, M., N. Nikolic, L. Kostic, J. Pavlovic, P. Bosnic, N. Stevic, J. Savic and N. Hristov. 2016. The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition. Sci. Total Environ. 553:141–148. Novoselec, J., Ž. Klir, M. Domaćinović, Z. Lončarić and Z. Antunović. 2018. Biofortification of feedstuffs with microelements in animal nutrition. Agriculture. 24:25-34. Owusu-Sekyere, A., J. Kontturi, R. Hajiboland, S. Rahmat, N. Aliasgharzad, H. Hartikainen and M.M. Seppänen. 2013. Influence of selenium (Se) on carbohydrate metabolism, nodulation and growth in alfalfa (Medicago sativa L.). Plant Soil. 373:541–552. Petrujkić, B.T., D.S. Šefer, I.B. Jovanović, M. Jovičin, S. Janković, G. Jakovljević, R.C. Beier and R.C. Anderson. 2014. Effects of commercial selenium products on glutathione peroxidase activity and semen quality in stud boars. Anim. Feed Sci. Techno. 197:194–205. Qin, S., J. Gao and K. Huang. 2007. Effects of different selenium sources on tissue selenium concentrations, blood GSH-Px activities and plasma interleukin levels in finishing lambs. Biol Trace Elem Res. 116:91–102. Radović, J., D. Sokolović and J. Marković. 2009. Alfalfa-most important perennial forage legume in animal husbandry. Biotechnol. Anim. Husb. 25: 465–475. Ros, G. H., A.M.D. Van Rotterdam, D.W. Bussink and P.S. Bindraban. 2016. Selenium fertilization strategies for bio-fortification of food: an agro-ecosystem approach. Plant Soil. 404:99–112. Sajedi, N.A., M.R. Ardakani, H. Madani, A. Naderi and M. Miransari. 2011. The effects of selenium and other micronutrients on the antioxidant activities and yield of corn (Zea mays L.) under drought stress. Physiol. Mol. Biol. Plants. 17:215–222. Slavik, P., J. Illek, M. Brix, J. Hlavicova, R. Rajmon and F. Jilek. 2008. Influence of organic versus inorganic dietary selenium supplementation on the concentration of selenium in colostrum, milk and blood of beef cows. Acta Vet. Scand. 50:43. Suttle, N.F. 2010. Mineral Nutrition of Livestock. 4th ed. Wallingford: CABI Publishing. Terry, N., A.M. Zayed, M.P. De Souza and A.S. Tarun. 2000. Selenium in higher plants. Annu Rev Plant Biol. 51:401–432. Tremblay, G.F., G. Bélanger, J. Lajeunesse, P.Y. Chouinard and É. Charbonneau. 2015. Timothy response to increasing rates of selenium fertilizer in Eastern Canada. Agron J. 107:211–220. Tucak, M., S. Popović, T. Čupić, G. Krizmanić, V. Španić, B. Šimić and V. Meglič. 2014. Agro-Morphological and forage quality traits of selected alfalfa populations and their application in breeding. Turk J Field Crop. 19(1):79-83. Wang, J., Z. Wang, H. Mao, H. Zhao and D. Huang. 2013. Increasing Se concentration in maize grain with soil-or foliar-applied selenite on the Loess Plateau in China. Field Crops Res. 150:83–90. Yruela, I. 2005. Copper in plants. Braz. J. Plant Physiol. 17:145–156.