DEVELOPMENT OF PVX RESISTANT POTATO BREEDING LINES USING MARKER-ASSISTED SELECTION

Abstract

Potato breeding requires a long and laborious study as a based on phenotypic and genotypic selection studies due to its tetraploid and heterozygous plant structure. This research was made to show that the variety development can be faster with molecular assisted breeding. In this research, a short breeding program was applied in order to develop new varieties by taking high dry matter content and PVX resistance as the main criterion. The research was carried out between 2014-2019 and 16.000 F1 potato seeds were used as a genetic material. A hard and effective selection breeding, breeder field observation and PVX markers based on tuber and plant characteristics were selected. In this research, fresh market, French fry and chips variety candidates which have high dry matter content, PVX resistant and higher tuber yield compared to commercial varieties have been developed. According to the results of the research; special breeding programs with two selection criteria can be used to shorten the potato breeding time and commercial candidate varieties with superior properties can be developed. Superior lines that showed to be carrying the markers are strongly recommended to be used by breeders to develop new PVX extreme resistance potato varieties.

Keywords

• Advanced line,genetic,potato clones,Solanum tuberosum L.,tuber yield

References

1. Ahmadvand, R., I. Wolf, A.M. Gorji, Z. Polgár and J. Taller. 2013. Development of molecular tools for distinguishing between the highly similar Rx1 and Rx2 PVX extreme resistance genes in tetraploid potato. Pot. Res. 56: 277-291.
2. Asano, K., A. Kobayashi, S. Tsuda, M. Nishinaka and S. Tamiya. 2012. DNA marker-assisted evaluation of potato genotypes for potential resistance to potato cyst nematode pathotypes not yet invading into Japan. Breeding Science. 62: 142-150.
3. Asmamaw, Y., T. Tekalign and T.S. Workneh. 2010. Specific gravity, dry matter concentration, pH and crisp-making potential of Ethiopian potato L.) cultivars as influenced by growing environment and length of storage under ambient conditions. Pot. Res. 53: 95-109.
4. Barone, A. 2004. Molecular marker-assisted selection for potato breeding. Amer. Jour. Pot. Res. 81: 111-117.
5. Bradshaw, J.E. and G.R. Mackay. 1994. Breeding strategies for clonally propagated potatoes. In: Bradshaw JE, Mackay GR (eds) Potato Genetics. Cab International, Wallingford, pp 467- 497.
6. Bradshaw J. 2007. The canon of potato science: four tetrasomic inheritance. Potato Res 50:219-222.
7. Carputo, D., and L.Frusciante. 2011. Classical Genetics and Traditional Breeding. In: Bradeen, J.M., Kole, C. (Eds.) Genetics, Genomics and Breeding of Potato. Published by Science Publishers. Enfield, NH 03748, USA, 20-40.
8. Ekin, Z. 2009. Determination of yield and quality characters of some potato (Solanum tuberosum L.) varieties under Ahlat ecological conditions. J. Agric. Fac. HRU. 13(3): 1-10 (In Turkish).

9. Freed, R., S.P. Einensmith, S. Guetz, D. Reicosky, V.W. Smail and P. Wolberg. 1989. User’s Guide to MSTAT-C Analysis of Agronomic Research Experiments. Michigan State Univ. USA.
10. Fulladolsa, A.C., F.M. Navarro, R. Kota, K. Severson, J.P. Palta and A.O. Charkowski. 2015. Application of marker assisted selection for potato virus Y resistance in the University of Wisconsin Potato Breeding Program. Amer. Jour. Pot. Res. 92: 444-450.
11. Gebhardt C, D. Bellin, H. Henselewski, W. Lehmann, J. Schwarzfischer and J.P.T. Valkonen. 2006. Marker assisted combination of major genes for pathogen resistance in potato. Theor. Appl. Genet. 112:1458-1464.
12. Gegov, Y., G. Pevicharova, E. Nacheva and V. Slavchev. 2007. Potato Breeding Lines Suitable For Production of Frozen French Fries. Bulgarian J. of Agric. Sci. 13: 15-29.
13. Haase, N.U. 2003. Estimation of dry matter and starch concentration in potatoes by determination of under-water weight and near infrared spectroscopy. Pot. Res. 46(4): 117- 127.
14. Haverkort, A. 2007. Potato crop response to radiation and daylength. In: Vreugdenhil D et al., editors. Potato Biology and Biotechnology. Amsterdam: Elsevier Science B.V.; p. 353-365.
15. Jansky, S. 2009. Breeding, genetics and cultivar development. In: Singh J, Kaur L (eds) Advances in potato chemistry and technology. Academic Press, New York, pp 27–62.
16. Kirkman, M. 2007. Global markets for processed potato products. In: Vreugdenhil D et al., editors. Potato Biology and Biotechnology. Amsterdam: Elsevier Science B.V. p. 27-44.
17. Kreuze, F., J.A.C. Sousa-Dias, A. Jeevalatha, A.R. Figueria, J.P.T. Valkonen, R.A.C. Jones. 2020. Viral Diseases in Potato. The Potato Crop. Its Agricultural, Nutritional and Social Contribution to Humankind. H. Campos, O. Ortiz (eds.). Springer, 389-430.
18. Kumari, M., M. Kumar, and S.S. Solanke. 2018. Breeding Potato for Quality Improvement. In book: Potato - From Incas to All Over the World. Chapter 3: 37-59.
19. Mehta, A., P. Charaya and B.P. Singh. 2011. French fry quality of potato varieties: effect of tuber maturity and skin curing. Potato J. 38 (2): 130-136.
20. Mori K, Y. Sakamoto, N. Mukojima, S. Tamiya, T. Nakao, T. Ishii and K. Hosaka. 2011. Development of a multiplex PCR method for simultaneous detection of diagnostic DNA markers of five disease and pest resistance genes in potato. Euphytica. 180: 347-355.
21. Nie X, V.L. Dickison, S. Brooks, B. Nie, M. Singh, D.L. De Koeyer, A.M. Murphy. 2018. High Resolution DNA Melting Assays for Detection of Rx1 and Rx2 for High-Throughput Marker-Assisted Selection for Extreme Resistance to Potato virus X in Tetraploid Potato. Plant Disease. 102(2): 382-390.
22. Ohbayashi K, N. Nakato, M. Chaya M and K. Kamura. 2010. Development of a detection method of resistance to potato disease and pest using DNA markers. I detection methods of resistance to potato virus X, potato cyst nematode and late blight. Bulletin Nagasaki Agriculture, Forest and Technology Development Center. p. 1-26.
23. Ozkaynak, E., Y. Orhan and T. Simsek. 2018. Determination of yield performance of early and main season potato commercial candidate varieties. Fresenius Environmental Bulletin. 27(5): 3087-3093.
24. Ozturk, G. and Z. Yildirim. 2011. Uniformity of Potato Minitubers Derived from Meristem Cultures of Nuclear Seed Stocks. Turkish Journal of Field Crops. 16(2): 149-152.
25. Ozturk, G. and Z. Yildirim. 2011. Uniformity of Potato Minitubers Derived from Meristem Cultures of Nuclear Seed Stocks. Turkish Journal of Field Crops. 16(2): 149-152.
26. Pandey, S.K., S.V. Singh, R.S. Marwaha and D. Pattanayak. 2009. Indian potato processing varieties: their impact and future priorities. Potato J. 36: 95-114.
27. Parlar, H., O. Gschwendtner, A. Anschutz, G. Leupold, and A. Gorg. 2001. Influence of selected parameters on the 2 isoelectric adsorptive bubble separation (iabs) of potato proteins. Advances in Food Sciences. 23(1): 1-10.
28. Pawelzik, E., E. Delgado, J. Poberezny and I. Rogoziňska. 1999. Effect of different climatic conditions on quality of certain German and Polish potato varieties. Abstract 14th Trien. Conference EAPR, Sorrento. p. 635-636.
29. Peleman, J.D. and J.R. der Voort. 2003. Breeding by design. Trends Plant Sci. 8: 330-334.
30. Shaikhaldein, H.O, B. Hoffmann I. A. Alaraidh D.G. Aseel. 2018. Evaluation of extreme resistance genes of Potato virus X (Rx1 and Rx2) in different potato genotypes. Journal of Plant Diseases and Protection. 125 (1-2): 1-7.
31. Slater, A.T., N.O.L. Cogan, B. Hayes, L., Schultz, M.F.B. Dale, G.J. Bryan and J. W. Forster. 2014. Improving breeding efficiency in potato using molecular and quantitative genetics. Theor. Appl. Genet. 127: 2279-2292.
32. Storey, M. 2007. The harvested crop. In: Vreugdenhil D et al., editors. Potato Biology and Biotechnology. Amsterdam: Elsevier Science B.V. p. 441-470
33. Tarn, T.R., G.C.C. Tai, H. De Jong, A.M. Murphy and J.E.A. Seabrook. 1992. Breeding potatoes for long-day, temperate climates. Janick J, John Wiley & Sons editors. In Plant Breeding Reviews. 9: 217-332.
34. Tekalign, T. and P.S. Hammes. 2005. Growth and productivity of potato as influenced by cultivar and reproductive growth: II. Growth analysis, tuber yield and quality. Scientia Horticulturae. 5: 29-44.
35. Yilmaz, H.A. and L. Gulluoglu. 2002. Determination of agronomic and quality traits of some potato (L.) varieties growing Harran plain. III. Nation Potato Congress, 23-27 September 2002, Izmir, Turkey, p. 179-192 (In Turkish).