QTL MAPPING OF BROOMRAPE (OROBANCHE CUMANA WALLR.) RESISTANCE IN SUNFLOWER (HELIANTHUS ANNUUS L.) USING GBS-SNPS

Year 2021, Volume: 26 Issue: 2, 157 - 162, 27.12.2021

Asena Akkose Baytar İbrahim Çelik Cafer Doğanlar Anne Frary Sami Doğanlar

https://doi.org/10.17557/tjfc.940409

Cited By: 1

Abstract

Broomrape is one of the most important biotic stresses causing serious yield reductions in sunflower. Control of this parasitic plant is difficult and physical and chemical strategies are usually insufficient. Therefore, introduction of genetic resistance to broomrape in sunflower is a key breeding goal. Breeding efforts on broomrape resistance have been conducted for decades, however, new broomrape races, such as race F, have emerged and rapidly evolved to be more aggressive and devastating. Although a few quantitative trait loci (QTLs) were identified for race F resistance, none of these loci are suitable for marker assisted selection because of their small phenotypic effects. In the present study, three major QTLs for broomrape race F resistance were identified on LG7, LG11 and LG12 using a high density SNP map constructed with the genotyping by sequencing approach in an intraspecific F2 population. The population consisted of 300 individuals derived from a cross between susceptible Helianthus annuus cv. RHA 436 as the recipient parent and resistant H. annuus cv. H08 M1 as the donor parent. Breeder-friendly SNP-based cleaved amplified polymorphic sequence markers were developed for the QTLs. The QTLs and CAPS markers identified in this study will be valuable molecular genetic tools for sunflower breeding.

Keywords

Genotyping by sequencing, Sunflower, Orobanche, QTL, SNP

Supporting Institution

The Scientific and Technological Research Council of Turkey (TUBITAK).

Project Number

513O037

References

• Akhtouch, B., Del Moral, L., Leon, A., Velasco, L., Fernández-Martínez, J.M., Pérez-Vich, B. 2016. Genetic study of recessive broomrape resistance in sunflower. Euphytica. 209:419-428.
• Akhtouch, B., Muñoz‐Ruz, J., Melero‐Vara, J., Fernández‐Martínez, J., Domínguez, J. 2002. Inheritance of resistance to race F of broomrape in sunflower lines of different origins. Plant Breed. 121:266-268.
• Bulbul, A., Salihoglu, M., Sari, C., Aydin, A. 1999. Determination of broomrape (Orobanche cumana Wallr.) races of sunflower in the Thrace region of Turkey. Helia. 14:21-26.
• Celik, I., Bodur, S., Frary, A., Doganlar, S. 2016a. Genome-wide SNP discovery and genetic linkage map construction in sunflower (Helianthus annuus L.) using a genotyping by sequencing (GBS) approach. Mol. Breed. 36:133.
• Celik, I., Sogut, M. A., Ozkaynak, E., Doganlar, S., Frary, A. 2016b. Physical mapping of NBS-coding resistance genes to the Me-gene cluster on chromosome P9 reveals markers tightly linked to the N gene for root-knot nematode resistance in pepper. Mol. Breed. 36:137.
• Churchill, G.A., Doerge, R.W. 1994. Empirical threshold values for quantitative trait mapping. Genet. 138:963–971.
• Conesa, A., Götz, S., García-Gómez, J.M., Terol, J., Talón, M., Robles, M. 2005. Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics. 21:3674–3676.
• Cvejić, S., Radanović, A., Dedić, B., Jocković, M., Jocić, S., Miladinović, D. 2020. Genetic and genomic tools in sunflower breeding for broomrape resistance. Genes 11:152.
• Dong, Q., Schlueter, S.D., Brendel, V. 2004. PlantGDB, plant genome database and analysis tools. Nucleic Acids Res. 32:354-359.
• Duriez, P., Vautrin, S., Auriac, M.C., Bazerque, J., Boniface, M.C., Callot, C., et al. 2019. A receptor-like kinase enhances sunflower resistance to Orobanche Cumana. Nat. Plant. 5:1211.
• Fernández-Martínez, J., Pérez-Vich, B., Akhtouch, B., Velasco, L., Muñoz-Ruz, J., Melero-Vara, J.M., et al. 2004. Registration of Four Sunflower Germplasms Resistant to Race F of Broomrape. Crop Sci. 44:1033–1034.
• Grenz, J., Ikstoc, V., Manschadi, A., Sauerborn, J. 2008. Interactions of sunflower (Helianthus annuus) and sunflower broomrape (Orobanche cumana) as affected by sowing date, resource supply and infestation level. Field Crops Res. 107:170.
• Imerovski, I., Dimitrijević, A., Miladinović, D., Dedić, B., Jocić, S., Tubić, N.K., Cvejić, S. 2016. Mapping of a new gene for resistance to broomrape races higher than F. Euphytica. 209:281-289.
• Joehanes, R. and Nelson, J.C. 2008. QGene 4.0 an extensible Java QTL-analysis platform. Bioinformatics. 24:2788–2789.
• Kaya, Y., Evci, G., Pekcan, V., Gucer, T. 2004. Determining new broomrape-infested areas, resistant lines and hybrids in Trakya region in Turkey. Helia. 40:211–218.
• Knoth, C. and Eulgem, T. 2008. The oomycete response gene LURP1 is required for defense against Hyaloperonospora parasitica in Arabidopsis thaliana. Plant J. 55(1):53-64.
• Letousey, P., De Zélicourt, A., Vieira Dos Santos, C., Thoiron, S., Monteau, F., Simier, P., Thalouarn, P., Delavault, P. 2007. Molecular analysis of resistance mechanisms to Orobanche cumana in sunflower. Plant Pathol. 56:536–546.
• Li, J. and Timko, M.P. 2009. Gene-for-gene resistance in Striga-cowpea associations. Science. 325:1094–1094.
• Louarn, J., Boniface, M.C., Pouilly, N., Velasco, L., Pérez-Vich, B., Vincourt, P., Muños, S. 2016. Sunflower resistance to broomrape (Orobanche cumana) is controlled by specific QTLs for different parasitism stages. Front. Plant Sci. 7:590.
• Martín-Sanz, A., Malek, J., Fernández-Martínez, J.M., Pérez-Vich, B., Velasco, L. 2016. Increased virulence in sunflower broomrape (Orobanche cumana Wallr.) populations from Southern Spain is associated with greater genetic diversity. Front. Plant Sci. 7:589.
• Martin-Sanz, A., Perez-Vich, B., Rueda, S., Fernandez-Martinez, J.M., Velasco, L. 2020. Characterization of post-haustorial resistance to sunflower broomrape. Crop Sci. 60:1188-1198.
• Molinero-Ruiz, L., Garcia-Carneros, A.B., Collado-Romero, M., Raranciuc, S., Dominguez, J., Melero-Vara, M. 2014. Pathogenic and molecular diversity in highly virulent populations of the parasitic weed Orobanche Cumana (sunflower broomrape) from Europe. Weed Res. 54, 87–96.
• Pérez-Vich, B., Akhtouch, B., Knapp, S.J., Leon, A.J., Velasco, L., Fernández-Martínez, J.M., Berry, S.T. 2004. Quantitative trait loci for broomrape (Orobanche cumana Wallr.) resistance in sunflower. Theor. Appl. Genet. 109:92-102.
• Velasco, L., Pérez‐Vich, B., Jan, C.C., Fernández‐Martínez, J.M. 2007. Inheritance of resistance to broomrape (Orobanche cumana Wallr.) race F in a sunflower line derived from wild sunflower species. Plant Breed. 126:67-71.
• Vranceanu, A.V., Pirvu, N., Stoenescu, F.M., Pacureanu, M. 1986. Some aspects of the interactions Helianthus annuus L./Orobanche cumana Wallr. and its implications in sunflower breeding. pp: 181– 189. In S.J. ter Borg (ed.) Biology and control of Orobanche: Proc. Workshop in Wageningen, The Netherlands. Wageningen Agric Univ, Wageningen.
• You, F.M., Huo, N., Gu, Y.Q., Luo, M.C., Ma, Y., Hane, D., Lazo, G.R., Dvorak, J., Anderson, O.D. 2008. BatchPrimer3: a high through-put web application for PCR and sequencing primer design. BMC Bioinformatics 9:2.