SELECTION OF ALFALFA (Medicago sativa L.) HYBIRD PARENTS AND HETEROSIS ANALYSIS OF F1 HYBRIDS

Year 2022, Volume: 27 Issue: 2, 235 - 241, 23.12.2022

Shi Ruru Jie Yang Yanting Yang Zhang Yutong Fengling Shi

https://doi.org/10.17557/tjfc.1126296

Abstract

Heterosis is an effective way to increase yield and improve quality in alfalfa breeding. The key issue for efficiently use of heterosis is to identify parents having potential for producing hybrid combinations with higher performance. This study aimed to analyze genetic distance between the 48 alfalfa accessions, and combine different hybrid combinations. The results showed that the mating ability of the hybrid combination in which both parents are tetraploid is greater than that in which both parents are diploid or the hybrid combination with different parental ploidy. When the different ploidy levels of parents, the mating ability of alfalfa hybrid combination with diploid as female parent is better than that of alfalfa hybrid combination with tetraploid as female parent. he hybrid combination of diploid alfalfa as a female parent with tetraploid as a male parent is more heterosis than the hybrid combination of diploid as a male parent. Moreover, CYK2ｘHH10 with high heterosis and CYK4xHH10 with high the relative seed setting rate should be possible to breed high yielding hybrid, which could be used as candidate materials for further research.

Keywords

Alfalfa, Genetic distance, Seed setting; Heterosis

Supporting Institution

Key Laboratory of Grassland Resources of Ministry of Education of China. the National Natural Science Foundation of China

Project Number

32160323

References

• Li-Jun, X. U., G. X. Yang, B. R. Chen, X. P. Xin, Wuenqi, Qinggele and S. S. Zhu. 2013. Comprehensive evaluation of nutritional values of different alfalfa species and varieties. Pratacultural Science 30(04): 566-570.
• Groose, R. W., L. E. Talbert, W. P. Kojis and E. T. Bingham. 1989. Progressive Heterosis in Autotetraploid Alfalfa: Studies Using Two Types of Inbreds. Crop Science 29(5). Labroo, M. R., A. J. Studer and J. E. Rutkoski. 2021.Heterosis and Hybrid Crop Breeding: A Multidisciplinary Review. Front Genet 12: 643-761.
• Herrmann, D., S. Flajoulot, P. Barre, C. Huyghe and B. Julier. 2017 . Comparison of morphological traits and molecular markers to analyse diversity and structure of alfalfa (Medicago sativa L.) cultivars. Genetic Resources and Crop Evolution: 1-14.
• Geng, X., Y. Qu, Y. Jia, S. He, Z. Pan, L. Wang and X. Du. 2021. Assessment of heterosis based on parental genetic distance estimated with SSR and SNP markers in upland cotton (Gossypium hirsutum L.). BMC Genomics 22(1): 123.
• Jordan, D. R., Y. Tao, I. D. Godwin, R. G. Henzell, M. Cooper and C. L. McIntyre .2003. Prediction of hybrid performance in grain sorghum using RFLP markers. Theor Appl Genet 106(3): 559-567.
• Han, Z., Q. Han, Y. Xia, X. Geng, K. Du, J. Yang and X. Kang .2020 . Construction of a breeding parent population of Populus tomentosa based on SSR genetic distance analysis. Sci Rep 10(1): 18573.
• Mukri, G., M. S. Patil, B. N. Motagi, J. S. Bhat, C. Singh, S. P. Jeevan Kumar, R. N. Gadag, N. C. Gupta and J. Simal-Gandara. 2022. Genetic variability, combining ability and molecular diversity-based parental line selection for heterosis breeding in field corn (Zea mays L.).Mol Biol Rep.
• Annicchiarico, P., Y. Wei, E. C. Brummer and O. A. Rognli. 2017. Genetic structure of putative heterotic populations of alfalfa. Plant Breeding 136(5): 671-678. He, C., Z. L. Xia, T. A. Campbell and G. R. Bauchan. 2009. Development and Characterization of SSR Markers and Their Use to Assess Genetic Relationships among Alfalfa Germplasms.Crop Science 49(6): 2176-2186.
• Rui, M. A., T. Yli-Mattila and S. Pulli. 2004. Phylogenetic relationships among genotypes of worldwide collection of spring and winter ryes (Secale cereale L.) determined by RAPD-PCR markers. Hereditas.
• Stewart, C. and L. E. Via. 1993. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. Biotechniques 14(5): 748-750. Yun-Tana, W. U., F. L. Shi, X. L. Xue, F. L. Shi and N. Cui. 2015 . Correlation Analysis of SSR Genetic Distance and Heterosis on Alfalfa. Journal of Plant Genetic Resources.
• Qian, Q. A., B. My, W. A. Chong, A. Jw, A. Ql, A. Jt, Z. C. Lin and F. B. Zhen. 2021 . Analysis of leaf morphology variation and genetic diversity via SRAP markers for near-threatened plant Acer truncatum - ScienceDirect. Global Ecology and Conservation.
• Rongxi, S., L. Furong, H. Ping and Z. Yongqi. 2016. Moderate Genetic Diversity and Genetic Differentiation in the Relict Tree Liquidambar formosana Hance Revealed by Genic Simple Sequence Repeat Markers. Frontiers in Plant Science 7.
• Singh, N., D. R. Choudhury, G. Tiwari, A. K. Singh, S. Kumar, K. Srinivasan, R. K. Tyagi, A. D. Sharma, N. K. Singh and R. Singh.2016. Genetic diversity trend in Indian rice varieties: an analysis using SSR markers. BMC Genetics 17.
• Kumar, R. V., Y. K. Tripathi, P. Shukla, S. P. Ahlawat and V. K. Gupta. 2009. Genetic diversity and relationships among germplasm of Jatropha curcas L. revealed by RAPDs.Trees 23(5): 1075-1079.
• Huang, C. Q., G. D. Liu, C. J. Bai, W. Q. Wang and J. Tang . 2014. Application of SRAP markers in the identification of Stylosanthes guianensis hybrids. Molecular Biology Reports 41(9): 5923-5929.
• Boeven, P., Y. Zhao, P. Thorwarth, F. Liu and T. Würschum. 2020. Negative dominance and dominance-by-dominance epistatic effects reduce grain-yield heterosis in wide crosses in wheat. Science Advances 6(24): eaay4897.
• Jing, S., P. Kryger, B. Markussen and B. Boelt. 2021. Pollination and Plant Reproductive Success of Two Ploidy Levels in Red Clover (Trifolium pratense L.).Front Plant Sci 12: 720069.
• Wang, G. Castiglione ,S. Zhang, J. 1994. Hybrid rice (oryza sativa l.): identification and parentage determination by rapd fingerprinting. Plant Cell Reports, 14(2-3), 112-115.
• Jagosz, B. 2011. The relationship between heterosis and genetic distances based on RAPD and AFLP markers in carrot. Plant Breeding 130(5): 574-579.
• Stupar, S. 2007. Allele-Specific Expression Patterns Reveal Biases and Embryo-Specific Parent-of-Origin Effects in Hybrid Maize. Plant Cell 19(8): 2391-2402.
• Wang, H. Chen, F. , Duan, L., Ye, Y. 2016. The crossability and progeny ploidyidentification of hybridization among the heteroploidincrape myrtle.Journal of Hunan Agricultural University (03):291-295.
• Rao, L. Yang,H. Guo,H. Duan,H. Chen, Y. 2014. AFLP Analysis of Genetic Differences amongAln aus with Different Ploidies. Bulletin of Botanical Research ,34(06):803-809.