CYTOTOXIC EFFECT OF GREEN SYNTHESIZED SILVER NANOPARTICLES WITH SALVIA OFFICINALIS ON MCF-7 HUMAN BREAST CANCER CELLS

Year 2021, Volume: 4 Issue: 3, 133 - 139, 27.12.2021

Şükriye Yeşilot , Soner Dönmez

Abstract

Alternative in vitro nanotechnological methods have been developed to conventional methods in the treatment strategies of breast cancer. Silver nanoparticles (AgNPs) from metallic nanoparticles, especially those amplified using the green synthesis method, show promise as a suitable anticancer candidate in the field of nanomedicine. The purposed of the investigation was to green synthesize and characterize of silver nanoparticles (So-AgNPs) by Salvia officinalis aqueous extract and evaluate their anticancer effect on metastatic breast cancer cell line MCF-7. The formation of So-AgNPs were characterized by scanning electron microscopy (SEM) with Energy dispersive X-ray (EDX) and UV-visible spectroscopy. The surface absorption peak of So-AgNPs was observed at 417 nm by the UV-Vis analysis. MCF-7 and CRL-4010 cells were treated with different concentrations (0-0.05 µg/mL) of AgNPs for 24 h. The cytotoxic effect of green synthesized AgNPs against MCF-7 and CRL-4010 cell lines was approved by MTT [3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyl tetrazolium bromide] assay. MTT results showed that So-AgNPs have higher cytotoxic activity in MCF-7 breast cancer cell lines than in CRL-4010 human breast epithelial cells (IC50 values 8.49 and 9.69 µg/mL, respectively). AgNPs synthesized via Salvia officinalis extract exhibited a significant cytotoxic effect on MCF-7 cell lines, demonstrating that they could be a potential antitumor agent in the treatment of metastatic breast cancer. However, further research is required to elucidate the mechanisms of action.

Keywords

Siver nanoparticles, MFC-7, Salvia officinalis

References

• Stewart BW, & Wild CP. World Cancer Report 2014, Geneva, Switzerland. World Health Organization, International Agency for Research on Cancer, WHO Press. 2015;16-81.
• Waks AG, Winer EP. Breast Cancer Treatment: A Review. JAMA. 2019;321(3):288–300.
• Jones SE. Metastatic Breast Cancer: The Treatment Challenge. Clinical Breast Cancer. 2008;8(3):224-233.
• Petit T, Wilt M, Velten M, Millon R, Rodier J, Borel C, Mors R, Haegele P, Eber M, Ghnassia JP. Comparative value of tumour grade, hormonal receptors, Ki-67, HER-2 and topoisomerase II alpha status as predictive markers in breast cancer patients treated with neoadjuvant anthracycline-based chemotherapy. Eur J Cancer 2004;40(2): 205-11.
• Clarke MA, Fisher J. Executable cancer models: successes and challenges. Nat Rev Cancer, 2020; 20(6):343–354.
• Logothetidis S. Nanotechnology in medicine: the medicine of tomorrow and nanomedicine. Hippokratia. 2006;10 (1):7–21.
• Beyene HD, Werkneh AA, Bezabh HK, Ambaye TG. Synthesis paradigm and applications of silver nanoparticles (AgNPs), a review. Sustainable Materials and Technologies. 2017;13:18-23.
• Saxena SK, Nyodu R, Kumar S, Maurya VK. Current Advances in Nanotechnology and Medicine. In NanoBioMedicine; Saxena S, Khurana S, Eds.; Springer, 2020; 978-981-32-9897-2.
• Andra S, Balu SK, Jeevanandham J, et al. Phytosynthesized metal oxide nanoparticles for pharmaceutical applications. Naunyn-Schmiedeberg's Arch Pharmacol. 2019; 392:755–771.
• Dönmez S. Green Synthesis of Zinc Oxide Nanoparticles Using Zingiber Officinale Root Extract and Their Applications in Glucose Biosensor. El-Cezeri Journal of Science and Engineering. 2020;7(3):1191-1200.
• Chandra H, Kumari P, Bontempi E, Yadav S. Medicinal plants: Treasure trove for green synthesis of metallic nanoparticles and their biomedical applications. Biocatalysis and Agricultural Biotechnology. 2020;24(1):101518.
• Salem SS, Fouda A. Green Synthesis of Metallic Nanoparticles and Their Prospective Biotechnological Applications: an Overview. Biological Trace Element Research. 2021;199: 344–370.
• Yadi M, Mostafavi E, Saleh B, Davaran S, Aliyeva I, Khalilov R, Nikzamir M, Nikzamir N, Akbarzadeh A, Panahi Y, Milani M. Current developments in green synthesis of metallic nanoparticles using plant extracts: a review. Artificial Cells, Nanomedicine, and Biotechnology. 2018;46(3):336-343.
• Rana A, Yadav K, Jagadevan S. A comprehensive review on green synthesis of nature-inspired metal nanoparticles: Mechanism, application and toxicity. Journal of Cleaner Production 2020;272:122880.
• Kumar V, Yadav SK. Plant‐mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol. 2009;84:151–157.
• Mohamad NAN, Arham NA, Jai J, Hadi A. Plant Extract as Reducing Agent in Synthesis of Metallic Nanoparticles: A Review. Advanced Materials Research. 2013;832:350–355.
• Nadaroglu H, Alaylı GA, İnce S. Synthesis of Nanoparticles by Green Synthesis Method. International Journal of Innovative Research and Reviews. 2017;1: 6-9.
• Kamran U, Bhatti HN, Iqbal M, Nazir A. Green synthesis of metal nanoparticles and their applications in different fields: a review. Zeitschrift für Physikalische Chemie. 2019;233(9):1325-1349.
• Lu Y, Foo LY. Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chemistry. 2001;75(2):197-202.
• Ghorbani A, Esmaeilizadeh M. Pharmacological properties of Salvia officinalis and its components. Journal of Traditional and Complementary Medicine. 2017;7(4):433-440.
• Akhtar M, Panwar J, Yun YS. Biogenic synthesis of metallic nanoparticles by plant extracts. ACS Sustain Chem Eng. 2013;1:591-602.
• Ahmad S, Munir S, Zeb N, Ullah A, Khan B, Ali J, Bilal M, Omer M, Alamzeb M, Salman SM, Ali S. Green nanotechnology: a review on green synthesis of silver nanoparticles - an ecofriendly approach. International journal of nanomedicine 2019;14:5087–5107.
• Yousaf H, Mehmood A, Ahmad KS, Raffi M. Green synthesis of silver nanoparticles and their applications as an alternative antibacterial and antioxidant agents, Materials Science and Engineering: C. 2020;112:110901.
• Lee SH, Jun B-H. Silver Nanoparticles: Synthesis and Application for Nanomedicine. International Journal of Molecular Sciences. 2019;20(4):865.
• Kuppusamy P, Yusoff MM, Maniam GP, Govindan N. Biosynthesis of metallic nanoparticles using plant derivatives and their new avenues in pharmacological applications – An updated report. Saudi Pharm J. 2016;24(4):473-84.
• Baharara J, Ramezani T, Mousavi M, Asadi-Samani M. Antioxidant and anti-inflammatory activity of green synthesized silver nanoparticles using Salvia officinalis extract. Ann Trop Med Public Health 2017;10:1265-70
• Okaiyeto K, Hoppe H. & Okoh AI. Plant-Based Synthesis of Silver Nanoparticles Using Aqueous Leaf Extract of Salvia officinalis: Characterization and its Antiplasmodial Activity. J Clust Sci. 2021;32:101–109
• Ramsden J. Essentials of nanotechnology. Nanotechnology Jeremy Ramsden & Ventus Publishing ApS, Denmark. 2009.
• Bayda S, Adeel M, Tuccinardi T, Cordani M, Rizzolio F. The History of Nanoscience and Nanotechnology: From Chemical–Physical Applications to Nanomedicine. Molecules. 2020; 25(1):112. https://doi.org/10.3390/molecules25010112
• Kulkarni N, Muddapur U. Biosynthesis of Metal Nanoparticles: A Review. Journal of Nanotechnology, 2014;510246:1-8.
• Bhardwaj K, Dhanjal DS, Sharma A, et al. Conifer-Derived Metallic Nanoparticles: Green Synthesis and Biological Applications. Int J Mol Sci. 2020;21(23):9028. doi:10.3390/ijms21239028
• Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials, Biotechnology Advances. 2009;27(1):76-83.
• Salleh A, Naomi R, Utami ND, et al. The Potential of Silver Nanoparticles for Antiviral and Antibacterial Applications: A Mechanism of Action. Nanomaterials (Basel). 2020;10(8):1566. doi:10.3390/nano10081566
• Mathur P, Jha S, Ramteke S, Jain NK. Pharmaceutical aspects of silver nanoparticles. Artificial Cells, Nanomedicine, and Biotechnology. 2017;12:1–12.
• Galatage ST, Hebalkar AS, Dhobale SV, Mali OR, Kumbhar PS, Nikade SV and Killedar SG. Silver Nanoparticles: Properties, Synthesis, Characterization, Applications and Future Trends, Silver Micro-Nanoparticles-Properties, Synthesis, Characterization, and Applications. IntechOpen. 2021. DOI: 10.5772/intechopen.99173.
• Poulose S, Panda T, Nair PP, Theodore T. Journal of Nanoscience and Nanotechnology, 2014;14(2):2038-2049(12).
• Moradi F, Sedaghat S, Moradi O & Salmanabadi SA. Review on green nano-biosynthesis of silver nanoparticles and their biological activities: with an emphasis on medicinal plants. Inorganic and Nano-Metal Chemistry. 2021;51(1):133-142.
• Makarov VV, Love AJ, Sinitsyna OV, et al. "Green" nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae. 2014;6(1):35-44.
• Miraj S, Kiani S. A review study of therapeutic effects of Salvia officinalis L. Der Pharmacia Lettre. 2016;8(6):299-303
• Martins N, Barros L, Santos-Buelga C, Henriques M, Silva S, Ferreira ICFR. Evaluation of bioactive properties and phenolic compounds in different extracts prepared from Salvia officinalis L. Food Chemistry. 2015;170:378-385.
• Jakovljević M, Jokić S, Molnar M, Jašić M, Babić J, Jukić H, Banjari I. Bioactive Profile of Various Salvia officinalis L. Preparations. Plants. 2019;8(3):55.
• Prabu HJ, Johnson I. Plant-mediated biosynthesis and characterization of silver nanoparticles by leaf extracts of Tragia involucrata, Cymbopogon citronella, Solanum verbascifolium and Tylophora ovata. Karbala International Journal of Modern Science. 2015;1(4):237-246.
• Wang M, Zhang W, Zheng X, Zhu P. Antibacterial and catalytic activities of biosynthesized silver nanoparticles prepared by using an aqueous extract of green coffee bean as a reducing agent. RSC Adv. 2017;7(20):12144–12149.
• Vishwanatha T, Keshavamurthy M, Mallappa M, Murugendrappa MV, Nadaf YF, Siddalingeshwara KG & Dhulappa A. Biosynthesis, characterization and antibacterial activity of silver nanoparticles from Aspergillus awamori. Journal of Applied Biology & Biotechnology. 2018; 6(5):12-16.
• Kgatshe M, Aremu OS, Katata-Seru L & Gopane R. Characterization and antibacterial activity of biosynthesized silver nanoparticles using the ethanolic extract of Pelargonium sidoides DC. Journal of Nanomaterials, vol. 2019, Article ID 3501234, 10 pages, 2019. https://doi.org/10.1155/2019/3501234
• Behboodi S, Baghbani-Arani F, Abdalan S, Sadat Shandiz SA. Green Engineered Biomolecule-Capped Silver Nanoparticles Fabricated from Cichorium intybus Extract: In Vitro Assessment on Apoptosis Properties Toward Human Breast Cancer (MCF-7). Cells. Biological Trace Element Research. 2018;187(2):392-402.
• Hamelian M, Zangeneh MM, Amisama A, Varmira K, Veisi H. Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Appl. Organometal Chem. 2018;32:4458.
• Aslany S, Tafvizi F, Naseh V. Characterization and evaluation of cytotoxic and apoptotic effects of green synthesis of silver nanoparticles using Artemisia Ciniformis on human gastric adenocarcinoma. Materials Today Communications. 2020;24:101011.
• Baharara J, Namvar F, Mousavi M, Ramezani T, Mohamad R. Anti-Angiogenesis Effect of Biogenic Silver Nanoparticles Synthesized Using Saliva officinalis on Chick Chorioalantoic Membrane (CAM). Molecules. 2014; 19(9):13498-13508.
• Sehnal K, Hosnedlova B, Docekalova M, Stankova M, Uhlirova D, Tothova Z, Kepinska M, Milnerowicz H, Fernandez C, Ruttkay-Nedecky B, Nguyen HV, Ofomaja A, Sochor J, Kizek R. An Assessment of the Effect of Green Synthesized Silver Nanoparticles Using Sage Leaves (Salvia officinalis L.) on Germinated Plants of Maize (Zea mays L.). Nanomaterials. 2019; 9(11):1550.
• Safdar M, Ozaslan M, Khailany RA, Latif S, Junejo Y, Saeed M, ... & Kanabe BO. Synthesis, characterization and applications of a novel platinum-based nanoparticles: catalytic, antibacterial and cytotoxic studies. Journal of Inorganic and Organometallic Polymers and Materials. 2020;30:2430–2439.
• Ovais M, Khalil AT, Raza A, Khan MA, Ahmad I, Islam NU, Saravanan M, Ubaid MF, Ali M, Shinwari ZK. Green synthesis of silver nanoparticles via plant extracts: beginning a new era in cancer theranostics. Nanomedicine. 2016;11(23): 3157-3177.
• Salman G, Pehlivanoglu S, Aydin Acar C et al. Anticancer Effects of Vitis vinifera L. Mediated Biosynthesized Silver Nanoparticles and Cotreatment with 5 Fluorouracil on HT-29 Cell Line. Biol Trace Elem Res. 2021. https://doi.org/10.1007/s12011-021-02923-8
• Al-Nuairi AG, Mosa KA, Mohammad MG, et al. Biosynthesis, Characterization, and Evaluation of the Cytotoxic Effects of Biologically Synthesized Silver Nanoparticles from Cyperus conglomeratus Root Extracts on Breast Cancer Cell Line MCF-7. Biol Trace Elem Res. 2020;194:560–569 https://doi.org/10.1007/s12011-019-01791-7
• Aydın Acar Ç, Pehlivanoğlu S. Gümüş Nanopartiküllerin Biberiye Özütü ile Biyosentezi ve MCF-7 Meme Kanseri Hücrelerinde Sitotoksik Etkisi. Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi. 2019;10 (2):172-176.
• Ullah I, Khalil AT, Ali M, Iqbal J, Ali W, Alarifi S and Shinwari ZK. Green-Synthesized Silver Nanoparticles Induced Apoptotic Cell Death in MCF-7 Breast Cancer Cells by Generating Reactive Oxygen Species and Activating Caspase 3 and 9 Enzyme Activities. Oxidative Medicine and Cellular Longevity. 2020;Article ID 1215395:14 https://doi.org/10.1155/2020/1215395
• Gomathi AC, Xavier Rajarathinam SR, Mohammed Sadiq A, Rajeshkumar S. Anticancer activity of silver nanoparticles synthesized using aqueous fruit shell extract of Tamarindus indica on MCF-7 human breast cancer cell line. Journal of Drug Delivery Science and Technology. 2020;55:101376, https://doi.org/10.1016/j.jddst.2019.101376.
• Emam M, El Raey MA, Eisa WH, El- Haddad AE, Osman SM, El-Ansari MA, Rabie AM. Green Synthesis of Silver Nanoparticles from Caesalpinia gilliesii (Hook) leaves: antimicrobial activity and in vitro cytotoxic effect against BJ-1 and MCF-7 cells. Journal of Applied Pharmaceutical Science 2017;7(08):226-233.
• Sharifi F, Sharififar F, Soltanian S, Doostmohammadi M, Mohamadi N. Synthesis of silver nanoparticles using Salvia officinalis extract: Structural characterization, cytotoxicity, antileishmanial and antimicrobial activity. Nanomedicine Research Journal. (2020);5(4):339-346. doi: 10.22034/nmrj.2020.04.005
• Yesilot S, Aydin CA. Silver nanoparticles; a new hope in cancer therapy?. Eastern Journal of Medicine. 2019;24(1):111-116.