Exopolysaccharide Production by Root Nodule Bacteria of Wild Lathyrus cassius Boiss.

Abstract

In this study, it was investigated that producing ability of extracellular polysaccharides (EPS) of the Rhizobium strains isolated from root nodules of wild Lathyrus cassius. The results provided that when isolates were grown in a chemically defined medium carbon and nitrogen sources itested in this study influced EPS synthesis. Maximum EPS production (1778 μg/ml and 1762.6 μg/ml, respectively) was obtained when the chemically defined medium was supplemented with mannitol and potassium nitrate, respectively which was accompanied a great increase in the production compared to the control. In all isolates, the maximum EPS production was 72 h, 150 rpm at mannitol and potassium nitrate containing medium.

Keywords

• Rhizobium sp.,isolates,exopolysaccharide,carbon and nitrogen source

Yabani Lathyrus cassius Boiss. Kök Nodül Bakterileri Tarafından Ekzopolisakkarit Üretimi

Öz

Çalışmada, yabani Lathyrus cassius’ün kök nodüllerinden izole edilen Rhizobium sp. izolatlarının ekzopolisakkarit (EPS) üretimleri araştırılmıştır. İzolatlar belirli bir kimyasal ortamda geliştirildiklerinde, test edilen karbon ve azot kaynakları EPS üretimini etkilemiştir. Ortama sırasıyla mannitol ve potasyum nitrat eklendiğinde kontrolle karşılaştırıldığında EPS üretiminin (sırasıyla, 1778 µg/ml ve 1762.6 µg/ml) maksimum değerde olduğu belirlenmiştir. İzolatların tümünde maksimum EPS üretimi 150 rpm’de 72 saatte, mannitol ve potasyum nitrat içeren ortamda bulunmuştur

Anahtar Kelimeler

• Rhizobium sp., izolatlar, ekzopolisakkarit, karbon ve azot kaynağı

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