Indole Acetic Acid Production by Rhizobium sp. Isolated from Pea (Pisum sativum L. ssp. arvense)

Abstract

In this study, isolates of the genus Rhizobium were isolated from root nodules of peas (Pisum sativum L. ssp. arvense) which were taken from pasture of Şanlıurfa, Turkey. Indole acetic acid (IAA) production by Rhizobium sp. was determined and IAA production of tested isolates ranged from 20.4 to 165.6 mg/ml. The highest IAA production and specific activity was found in isolate P2. Maximum IAA production for P2 isolate was obtained 96 h of inculbation time. IAA production by P2 isolate was determined in the media containing different carbon and nitrogen sources. Sucrose and glycine as carbon and nitrogen source, respectively gave the best result for P2 isolate.

Keywords

• Indole acetic acid,Rhizobium isolate,carbon and nitrogen sources

Bezelye (Pisum sativum L. ssp. arvense)’den İzole Edilen Rhizobium sp. Tarafından İndol Asetik Asit Üretimi

Öz

Bu çalışmada, Rhizobium cinsine ait izolatlar Türkiye, Şanlıurfa meralarından toplanan bezelyelerin (Pisum sativum L. ssp. arvense) kök nodüllerinden izole edilmiştir. Rhizobium sp. tarafından indol asetik asit (IAA) üretimi belirlenmiş ve test edilen izolatların IAA üretimi; 20.4-165.6 mg/ml arasında değişmiştir. En yüksek IAA üretimi ve spesifik aktivite P2 izolatında bulunmuştur. P2 izolatı için maksimum IAA üretimi inkübasyonun 96. saatinde incelenmiştir. P2 izolatı tarafından IAA üretimi farklı karbon ve azot kaynaklarının bulunduğu ortamlarda belirlenmiştir. Karbon ve azot kaynakları olarak sırasıyla; sukroz ve glisin, P2 izolatı için en iyi sonucu vermiştir.

Anahtar Kelimeler

• İndol asetik asit,Rhizobium izolatı,karbon ve azot kaynakları

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