Türkiye Florasında Doğal Olarak Yetişen Üç Farklı Vaccinium Türünün Sürgün Çoğaltımı Üzerine Besi ortamı pH’sının Etkisi

Öz

Besi ortamlarının pH’sı bitki hücre kültürlerini etkileyen en önemli faktörlerden biridir ve genellikle bunun kontrolüne çok az önem verilmiştir. Bu çalışma V. arctostaphylos, V. myrtillus ve V. uliginosum’un sürgün çoğaltımında besi ortamı pH değişimlerinin etkilerini belirlemek amacıyla dizayn edilmiştir. Sürgün çoğaltımı, sürgün sayısı, sürgün boyu, nod sayısı ve kallus oluşum yüzdeleri açısından değerlendirilmiştir. Bunun için pH’sı otoklavdan önce 4.0, 4.5, 5.0, 5.5 ve 6.0’a ayarlanmış ve 1.0/0.1 mg/L zeatin/IBA ile desteklenmiş WPM, temel besi ortamı olarak kullanılmıştır. Nod segmentlerinin eksplant olarak kullanıldığı in vitro çalışmalarda sürgün çoğaltımı besi ortamı pH’sının yanı sıra türe bağlı olarak da büyük ölçüde etkilenmiştir. Besi ortamlarının son pH değişimleri 3.66 ve 5.42 olarak belirlenmiştir. En yüksek sürgün sayısı eksplant başına 5.03 ile 5.5 pH içeren besi ortamdaki V. arctostaphylos’tan, en yüksek sürgün boyu ise 40.78 mm ile 4.5 pH içeren besi ortamdaki V. myrtillus’tan elde edilmiştir. V. arctostaphylos ve V. uliginosum için en yüksek nod sayısını sürgün başına 10.83 ile pH’sı 6.0 olan ortam vermiştir. V. arctostaphylos’da en yüksek sürgün boyu ve en yüksek nod sayısı açısından 6.0 pH daha üstün bulunurken, en yüksek sürgün sayısı için 5.5 pH daha uygun olmuştur. V. myrtillus’da tüm parametrelerde en yüksek değerler 4.5 pH içeren ortamdan elde edilmiştir. pH değerleri bir artış eğiliminde olduğunda V. uliginosum’da sürgün çoğaltım oranları önemli bir şekilde azalmıştır.

Anahtar Kelimeler

• Mikroçoğaltım,pH,Vaccinium arctostaphylos,V. myrtillus,V. uliginosum,zeatin

Effect of Medium pH on Shoot Regeneration of Three Vaccinium Species Naturally Growing in Turkish Flora

Abstract

Medium pH is one of the crucial factors affecting plant cell culture, and generally very little attention has been given to the control of it. The present study was designed to monitor the effects of pH changes on the course of shoot regeneration of Vaccinium arctostaphylos L., Vaccinium myrtillus L. and Vaccinium uliginosum L. Shoot regeneration was assessed by evaluating shoot numbers, shoot length, nod numbers, and callus formation. For this, McCown Woody Plant Medium (WPM) supplemented with 1.0/0.1 mg/L zeatin/indole-3- butyric acid (IBA) was employed as basal media being adjusted with initial pH at 4.0, 4.5, 5.0, 5.5 and 6.0 before autoclaving. When in vitro established nodal segments were employed, shoot regeneration was dramatically influenced depending on the species as well as the medium pH. The final pH changes were determined 3.66 and 5.42. The highest shoot number was obtained 5.03 (shoots per explant) at pH 5.5 in V. arctostaphylos whereas the highest shoot length (in average) was obtained (40.78 mm) at pH 4.5 pH in V. myrtillus. Both media having a pH of 6.0 gave the highest nodes number with 10.83 (nodes/explant) for V. arctostaphylos and V. uliginosum. In V. arctostaphylos, pH 5.5 was the most favorable for shoot number while pH 6.0 was found to be superior to the shoot length and nod number. In V. myrtillus, the highest values for all parameters were obtained at pH 4.5. When the pH is fluctuated tending an increase, the shoot multiplication rates significantly decreased in V. uliginosum.

Keywords

• Micropropagation,pH,Vaccinium arctostaphylos,V. myrtillus,V. uliginosum,zeatin

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