Daily Dose Dependent Effects of Fluoxetine-HCl (FLX) on Heart Tissue of Zebrafish (Danio rerio Hamilton, 1822)

Abstract

The aim of this study is to investigate the daily dose dependent effects of Fluoxetine-HCl (FLX) on antioxidant enzymes (CAT, GSH), lipid peroxidation levels (MDA) and total protein (TP) levels of zebrafish heart tissue. FLX is the active compound of the antidepressant Prozac™. FLX acts as a selective serotonin reuptake inhibitor (SSRI) in humans. This study was planned as a model to investigate the dose dependent effects of daily intake doses of FLX, which adapted from human proportionally to weight of zebrafish. Biochemical analyses catalase (CAT), glutatyon (GSH), lipid peroksidasyon levels (MDA) and total protein (TP) were detected using spectrophotometric methods. In this study, there was not found any negative effects of FLX on catalase and glutation activity, malondialdehyde levels and total protein levels of zebrafish heart tissue during specified periods.

Keywords

• Danio rerio,Fluoxetin-HCl,Heart tissue,Zebrafish

Fluoksetin-HCl’in (FLX) Zebra Balığı (Danio rerio Hamilton, 1822) Kalp Dokusu Üzerine Günlük Doza Bağlı Etkileri

Öz

Bu çalışmanın amacı, Fluoksetin-HCl’in (FLX) zebra balığı kalp dokusunda antioksidan enzimler (CAT, GSH), lipid peroksidasyon (MDA) ve total protein (TP) düzeyleri üzerine insanlarda kullanılan günlük doza bağlı etkilerinin araştırılmasıdır. FLX, insanlarda antidepresan olarak kullanılan Prozac™’ın etken maddesi ve SSRI (Selective Serotonine Reuptake Inhibitor) olarak bilinen bir serotonin geri alım inhibitörüdür. Bu çalışmada FLX’in günlük uygulama dozu zebra balıklarına adapte edilmiştir. Biyokimyasal analizler olan katalaz (CAT), glutatyon (GSH), lipid peroksidasyon düzeyleri (MDA) ve total protein (TP) miktarları spektrofotometrik yöntemlerle belirlenmiştir. Bu çalışmada FLX’in belirlenen zaman aralıklarında zebra balığı kalp dokusu katalaz ve glutatyon aktivitesi, malondialdehit seviyeleri ve total protein düzeyleri üzerine olumsuz bir etkisi saptanmamıştır. 

Anahtar Kelimeler

• Danio rerio,Fluoksetin-HCl,Kalp dokusu,Zebra balığı

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