LPS ile Oluşturulan Sepsisli Sıçanlarda Metformin ile Erken Tedavi, Pankreatik Hasarı Azaltır

Öz

Bu çalışmada, metforminin lipopolisakkarit (LPS) ile indüklenen sepsis sonrası pankreas dokusu üzerindeki etkilerinin araştırılması amaçlanmıştır. Çalışmada 30 adet Sprague Dawley cinsi erkek sıçan kullanılmıştır. Gruplar 5 adet ve her grupta 6 tane hayvan olacak şekilde; kontrol, sepsis, sepsis+1 saat önce metformin, sepsis+1 saat sonra metformin ve sepsis+3 saat sonra metformin olarak belirlenmiştir. LPS ve metformin dozları sırası ile 5 mg/kg ve 200 mg/kg olarak belirlenmiş olup hayvanlara intraperitoneal olarak uygulanmıştır. LPS enjeksiyonundan 24 saat sonra hayvanların kan örnekleri ve pankreas dokuları cerrahi işlemlerle alınmıştır. Sıçanların serumlarında amilaz, insülin ve glukoz düzeyleri ölçülmüştür. Alınan pankreas dokularında ise malondialdehit (MDA) ve myeloperoksidaz (MPO) düzeyleri ölçülmüştür. Ayrıca pankreas dokuları hematoksilen-eosin (H-E) boyama yöntemi ile histopatolojik olarak değerlendirilmiştir. Sonuçlar incelendiğinde, LPS’nin sıçanlarda sepsis meydana getirdiği ve pankreas dokularında hasar oluşturduğu görülmüştür. Ancak, metforminin tedavi gruplarında bu hasarı önemli ölçüde hafiflettiği tespit edilmiştir. Özellikle, sıçanlara sepsis oluşturulmadan önce uygulanan metforminin daha etkili olduğu, pankreas dokularında koruyucu etkilere sahip olduğu gösterilmiştir.

Anahtar Kelimeler

• Lipopolisakkarit,Metformin,Pankreas,Sepsis,Sıçan

Early Treatment with Metformin Decreases Pancreatic Damage in Rats with LPS Induced Sepsis

Abstract

In this study, the effects of metformin on pancreatic tissue after lipopolysaccharide (LPS)-induced sepsis were investigated. 30 Sprague Dawley male rats were used in the study. Five groups were formed: control, sepsis, sepsis+1 hour before metformin, sepsis+1 hour later metformin and sepsis+3 hour later metformin as 6 animals in each group. LPS and metformin was prepared at 5 mg/kg and 200 mg/kg volumes, respectively, and injected intraperitoneally to the rats. Blood samples and pancreas tissues were taken from the rats 24 hours after LPS injection. Amylase, glucose and insulin parameters were measured in serum of rats. Malondialdehyde (MDA) and myeloperoxidase (MPO) parameters in pancreas tissues of rats were evaluated. Pancreatic tissues were examined by hematoxylin-eosin (H-E) staining method histopathologically. When the results were evaluated, it was seen that LPS caused sepsis and pancreatic tissue damage in rats. However, it has been determined that metformin significantly alleviates these damages in the treatment groups. In particular, metformin administered prior to sepsis has been shown to have protective effects in the pancreatic tissues of rats.

Keywords

• Lipopolysaccharide,Metformin,Pancreas,Sepsis,Rat

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