DETERMINATION TO RESIDUAL N EFFECT OF LENTIL VARIETIES ON SUCCEEDING WHEAT N-15 ISOTOPE TRACING TECHNIQUE

Abstract

The field experiments were conducted to determine nitrogen fixing capacities of winter and summer lentil (Lens culinaris Medic.) varieties and the carry-over N effect on succeeding wheat, using N-15 isotope technique, under Central Anatolia dryland conditions. The use of labelled nitrogen provides a unique tool allowing to separately study the behaviour of fertilizer N, soil N and also to quantify the amount of biologically fixed nitrogen. First year, biological nitrogen fixation capacities of winter and summer lentil varieties were estimated by using N-15 methodology. Second year, carry-over of N from the legume residue to wheat was determined. According to first year results, the average of fixed nitrogen were found as 70.0 and 45.0 kgN/ha for winter and summer varieties, respectively. Second year, the average of carry-over N derived from soil values were found 43.0 and 17.0 kgN/ha for succeeding wheat when the previous legumes were winter and summer lentil varieties, respectively.

Keywords

• N-15 Isotope Technique,Lentil (Lens culinaris Medic.),Biological Nitrogen Fixation

References

1. Asfary AF, Khalifa KH, Janat M (1996). Production of wheat preceded with fallow versus continuous wheat or following chickpea or lentil in the Syrian Arab Republic. IAEA-TECDOC-875, p 23.
2. Bremer E, Rennie RJ, Rennie DA (1988). Dinitrogen fixation of lentil, field pea and fababean under dryland conditions. Canadian J. of Soil Science 68: 3, 553-562.
3. Chianu JN, Nkonya EM, Mairura FS, Justina NC, Akinnifesi FK (2011). Biological nitrogen fixation and socioeconomic factors for legume production in sub-Saharan Africa: a review. Agron Sustain Dev 31: 139–154.
4. Cowell LE, Bremer E, Kessel C van (1989). Yield and N2 fixation of pea and lentil as affected by intercropping and N application. Canadian J. of Soil Sci. 69:2, 243-251.
5. Faust H (1981). Training Manual: Interregional Training Course on the use of N-15 in soil science and plant nutrition, Zentralinstitut für Isotopen und Strahlenforschung, Leipzig.
6. Fried, M. and Broeshart, H. (1975). An independent measurement of the amount of nitrogenfixed by legume crops. Plant and Soil 43: 707-711.
7. Fried, M. and Broeshart, H. (1981). A further extension of the method for independentlymeasuring the amount of N fixed by a legume crop.Plant and Soil (62): 331-336.
8. Fried, M. and Middleboe, V. (1977). Measurement of amount of nitrogen fixed by a legumecrop. Plant and Soil 47: 713-715.

9. Halitligil MB, Akın A, Aydın M, Yılmaz A, Donmez O (1996)Effects of legumes, fallow and wheat on subsequent wheat production in central Anatolia. IAEA-TECDOC-875, p.53.
10. Halitligil MB, Akın A, Bilgin N, Deniz Y, Ogretir K, Altınel B, Isık Y (2000) Effect of nitrogen fertilization on yield and nitrogen and water use efficiencies of winter wheat (durum and bread) varieties grown under conditions found in Central Anatolia. Biology and Fertility of Soils, 31: 175-182.
11. Hardarson, G., Zapata, F. and Danso, S.K.A. (1984). Field evaluation of symbiotic N2 fixation by rhizobial strains using N-15 methodology. Plant and Soil 82: 369-375.
12. Hardarson G, Danso SKA, Zapata F (1988) N2 fixation measurements in alfalfa-ryegrass swards using N-15 and influence of the reference crop. Crop Sci. 28: 101-105
13. IAEA (1990) Training Course Series No.2. Use of nuclear techniques in studies of soil-plant relationships. International Atomic Energy Agency, Vienna.
14. Mcauliffe, C., Chamblee, D.S., Uribe-Arango, H. and Woodhouse, W.W. (1958). Influence of inorganic nitrogen or nitrogen fixation by legumes as revealed by N-15. Agronomy Journal 50: 334-337.
15. Senaratne R, Hardarson G (1988) Estimation of residual N effect of faba bean and pea on two succeeding cereals using N-15 methodology. Plant and Soil 110: 81-89.
16. Wagner GH, Zapata F (1982) Field evaluation of reference crops in the study of nitrogen fixation by legumes using isotope techniques. Agronomy J., 74: 607-612.
17. Unkovich MJ, Herridge D, Peoples M, Cadisch G, Boddey R, Giller K,Alves B, Chalk P (2008) Measuring plant-associated nitrogenfixation in agricultural systems. ACIAR Monograph No. 6, 258 pp.