Isolation and Identification of Entomopathogenic Bacteria from Some Species of Curculionidae Familia Harmful to Leguminous Forage Plants

Abstract

In this study; It is aimed to isolate and identify entomopathogenic bacterial microorganisms from some insects belonging to Curculionidae family which are harmful in leguminous forage plants in Erzurum province and some districts (Aşkale, Pasinler, Tortum and Oltu). Different periods of diseased and dead pest insects used in the research were collected in June and July from areas unused chemicals and brought to the laboratory under suitable conditions. Many isolates of bacteria have been obtained by isolating these samples brought from the laboratory. A total of 88 isolates from these isolates were isolated and stored at -80 ° C. Some classical and molecular methods Microbial Identification System (MIS) were used in the identification of each isolate of bacteria. When the identified isolates were examined, they were used as biopesticides in many studies. 3 Bacillus thuringiensis, 3 Bacillus pumilus, 4 Brevibacillus brevis, 4 Paenibacillus spp. And 2 Serratia spp., Were obtained. According to the results of classical and biochemical tests carried out with the aim of identifying bacterial isolates All isolates were positive for the catalase test and for KOH tests, both negative and positive results were obtained between the isolates. As a result of the hypersensitivity tests (HR) performed on tobacco plants, it was determined that the obtained isolates gave a negative result. As a result, entomopathogens obtained from this study were diagnosed in the determined target direction, It is thought that there are potentially usable species as biopesticides in isolates, in particular against some damages of the Curculionidae family which are harmful in leguminous forage plants.

Keywords

• Biopesticides,Leguminous forage plants

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