Barbaloin Attenuates Oxidative Testicular Injury Induced by Ischemia Reperfusion via Antioxidant Effects

Abstract

The purpose of this research is to examine the protective effects of barbaloin on testis injury induced by ischemia reperfusion. In the experimental stage of our research, the rats were assigned to 4 groups. Our groups are scheduled as follows; sham, ischemia reperfusion, ischemia reperfusion + DMSO and ischemia reperfusion+barbaloin. Some oxidant, antioxidant and inflammatory parameters were evaluated in testis tissues obtained at the end of the experiment. In ischemia reperfusion group it was found out that the oxidant parameters increased and antioxidant parameters decreased but on the contrary, the antioxidant parameters increased and oxidant parameters decreased in the treatment group. These results demonstrated that barbaloin administration performed a protective effect against oxidative testis injury induced by ischemia reperfusion.

Keywords

• ischemia reperfusion,barbaloin,testis,oxidative stress,inflamation

References

1. Asghari A, Akbari G, Meghdadi A, Mortazavi P. 2016. Protective effect of metformin on testicular ischemia/reperfusion injury in rats. Acta cirurgica brasileira 31:411-6
2. Abbas AM, Sakr HF. 2013. Effect of selenium and grape seed extract on indomethacin-induced gastric ulcers in rats. Journal of physiology and biochemistry 69:527-37
3. Ekici S, Dogan Ekici AI, Ozturk G, Benli Aksungar F, Sinanoglu O, et al. 2012. Comparison of melatonin and ozone in the prevention of reperfusion injury following unilateral testicular torsion in rats. Urology 80:899-906
4. Ringdahl E, Teague L. 2006. Testicular torsion. American family physician 74:1739-43
5. Ozturk H, Ozturk H, Gideroglu K, Terzi H, Bugdayci G. 2010. Montelukast protects against testes ischemia/reperfusion injury in rats. Canadian Urological Association journal = Journal de l'Association des urologues du Canada 4:174-9
6. Ekinci Akdemir FN, Yildirim S, Kandemir FM, Aksu EH, Guler MC, et al. 2019. The antiapoptotic and antioxidant effects of eugenol against cisplatin-induced testicular damage in the experimental model. Andrologia:e13353
7. Turner TT, Brown KJ. 1993. Spermatic cord torsion: loss of spermatogenesis despite return of blood flow. Biology of reproduction 49:401-7
8. Agarwal A, Virk G, Ong C, du Plessis SS. 2014. Effect of oxidative stress on male reproduction. The world journal of men's health 32:1-17

9. Turner TT, Tung KS, Tomomasa H, Wilson LW. 1997. Acute testicular ischemia results in germ cell-specific apoptosis in the rat. Biology of reproduction 57:1267-74
10. Agarwal A, Allamaneni SS. 2011. Free radicals and male reproduction. Journal of the Indian Medical Association 109:184-7
11. Doshi SB, Khullar K, Sharma RK, Agarwal A. 2012. Role of reactive nitrogen species in male infertility. Reproductive biology and endocrinology : RB&E 10:109
12. Durairajanayagam D, Agarwal A, Ong C, Prashast P. 2014. Lycopene and male infertility. Asian journal of andrology 16:420-5
13. Singab AN, El-Hefnawy HM, Esmat A, Gad HA, Nazeam JA. 2015. A Systemic Review on Aloe arborescens Pharmacological Profile: Biological Activities and Pilot Clinical Trials. Phytotherapy research : PTR 29:1858-67
14. Anuszewska EL. 2015. [Mechanisms of therapeutic action of aloe]. Wiadomosci lekarskie (Warsaw, Poland : 1960) 68:168-72
15. Akaberi M, Sobhani Z, Javadi B, Sahebkar A, Emami SA. 2016. Therapeutic effects of Aloe spp. in traditional and modern medicine: A review. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 84:759-72
16. Patel DK, Patel K, Tahilyani V. 2012. Barbaloin: a concise report of its pharmacological and analytical aspects. Asian Pacific journal of tropical biomedicine 2:835-8
17. Zhang P, Liu X, Huang G, Bai C, Zhang Z, Li H. 2017. Barbaloin pretreatment attenuates myocardial ischemia-reperfusion injury via activation of AMPK. Biochemical and biophysical research communications 490:1215-20
18. Halici Z, Karaca M, Keles ON, Borekci B, Odabasoglu F, et al. 2008. Protective effects of amlodipine on ischemia-reperfusion injury of rat ovary: biochemical and histopathologic evaluation. Fertil Steril 90:2408-15
19. Sahin FK, Cosar E, Koken G, Toy H, Basarali K, Buyukbas S. 2008. Protective effect of aprotinin on ischemia-reperfusion injury in rat ovary. J Obstet Gynaecol Re 34:794-800
20. Dogan C, Halici Z, Topcu A, Cadirci E, Karakus E, et al. 2016. Effects of amlodipine on ischaemia/reperfusion injury in the rat testis. Andrologia 48:441-52
21. Sun Y, Oberley LW, Li Y. 1988. A Simple Method for Clinical Assay of Superoxide-Dismutase. Clin Chem 34:497-500
22. Ohkawa H, Ohishi N, Yagi K. 1979. Assay for Lipid Peroxides in Animal-Tissues by Thiobarbituric Acid Reaction. Anal Biochem 95:351-8
23. Bradley PP, Priebat DA, Christensen RD, Rothstein G. 1982. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol 78:206-9
24. Burgher SW. 1998. Acute scrotal pain. Emergency medicine clinics of North America 16:781-809, vi
25. Anderson JB, Williamson RC. 1986. The fate of the human testes following unilateral torsion of the spermatic cord. British journal of urology 58:698-704
26. Dokmeci D. 2006. Testicular torsion, oxidative stress and the role of antioxidant therapy. Folia medica 48:16-21
27. Hausenloy DJ, Yellon DM. 2013. Myocardial ischemia-reperfusion injury: a neglected therapeutic target. J Clin Invest 123:92-100
28. Carden DL, Granger DN. 2000. Pathophysiology of ischaemia-reperfusion injury. The Journal of pathology 190:255-66
29. Zimmerman BJ, Granger DN. 1992. Reperfusion injury. The Surgical clinics of North America 72:65-83
30. Wang L, Liu X, Chen H, Chen Z, Weng X, et al. 2015. Effect of picroside II on apoptosis induced by renal ischemia/reperfusion injury in rats. Experimental and therapeutic medicine 9:817-22
31. Ayan M, Tas U, Sogut E, Cayli S, Kaya H, et al. 2016. Protective effect of thymoquinone against testicular torsion induced oxidative injury. Andrologia 48:143-51
32. Yulug E, Turedi S, Karaguzel E, Kutlu O, Mentese A, Alver A. 2014. The short term effects of resveratrol on ischemia-reperfusion injury in rat testis. Journal of pediatric surgery 49:484-9
33. Agarwal A, Nallella KP, Allamaneni SS, Said TM. 2004. Role of antioxidants in treatment of male infertility: an overview of the literature. Reproductive biomedicine online 8:616-27
34. Davies KJ. 2000. Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. IUBMB life 50:279-89
35. Li S, Tan HY, Wang N, Zhang ZJ, Lao L, et al. 2015. The Role of Oxidative Stress and Antioxidants in Liver Diseases. International journal of molecular sciences 16:26087-124
36. Filho DW, Torres MA, Bordin AL, Crezcynski-Pasa TB, Boveris A. 2004. Spermatic cord torsion, reactive oxygen and nitrogen species and ischemia-reperfusion injury. Molecular aspects of medicine 25:199-210
37. Rabus M, Demirbag R, Sezen Y, Konukoglu O, Yildiz A, et al. 2008. Plasma and tissue oxidative stress index in patients with rheumatic and degenerative heart valve disease. Turk Kardiyoloji Dernegi arsivi : Turk Kardiyoloji Derneginin yayin organidir 36:536-40
38. Erel O. 2005. A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry 38:1103-11
39. Yazici S, Demirtas S, Guclu O, Karahan O, Yavuz C, et al. 2014. Using oxidant and antioxidant levels to predict the duration of both acute peripheral and mesenteric ischemia. Perfusion 29:450-5
40. Yurtcu E, Togrul C, Ozyer S, Uzunlar O, Karatas YH, et al. 2015. Dose dependent protective effects of vardenafil on ischemia–reperfusion injury with biochemical and histopathologic evaluation in rat ovary. J Pediatr Surg 50:1205-9
41. El-Shemy HA, Aboul-Soud MA, Nassr-Allah AA, Aboul-Enein KM, Kabash A, Yagi A. 2010. Antitumor properties and modulation of antioxidant enzymes' activity by Aloe vera leaf active principles isolated via supercritical carbon dioxide extraction. Current medicinal chemistry 17:129-38
42. Jiang K, Guo S, Yang C, Yang J, Chen Y, et al. 2018. Barbaloin protects against lipopolysaccharide (LPS)-induced acute lung injury by inhibiting the ROS-mediated PI3K/AKT/NF-kappaB pathway. International immunopharmacology 64:140-50
43. Girotti AW. 1998. Lipid hydroperoxide generation, turnover, and effector action in biological systems. Journal of lipid research 39:1529-42
44. Gezici A, Ozturk H, Buyukbayram H, Ozturk H, Okur H. 2006. Effects of gabexate mesilate on ischemia-reperfusion-induced testicular injury in rats. Pediatric surgery international 22:435-41
45. Wang Z, Yu J, Wu J, Qi F, Wang H, et al. 2016. Scutellarin protects cardiomyocyte ischemia-reperfusion injury by reducing apoptosis and oxidative stress. Life sciences 157:200-7
46. Mullane KM, Kraemer R, Smith B. 1985. Myeloperoxidase activity as a quantitative assessment of neutrophil infiltration into ischemic myocardium. Journal of pharmacological methods 14:157-67