Isolation and Identification of Entomopathogenic Bacteria from Some Species of Curculionidae Familia Harmful to Leguminous Forage Plants

Year 2016, Volume: 1 Issue: 1, 20 - 27, 31.12.2016

Fatih Dadaşoğlu , Esin Dadaşoğlu

Abstract

In this study; It is aimed to isolate
and identify entomopathogenic bacterial microorganisms from some insects
belonging to Curculionidae family which are harmful in leguminous forage plants
in Erzurum province and some districts (Aşkale, Pasinler, Tortum and Oltu).
Different periods of diseased and dead pest insects used in the research were
collected in June and July from areas unused chemicals and brought to the
laboratory under suitable conditions. Many isolates of bacteria have been
obtained by isolating these samples brought from the laboratory. A total of 88
isolates from these isolates were isolated and stored at -80 ° C. Some
classical and molecular methods Microbial Identification System (MIS) were used
in the identification of each isolate of bacteria. When the identified isolates
were examined, they were used as biopesticides in many studies. 3 Bacillus
thuringiensis, 3 Bacillus pumilus, 4 Brevibacillus brevis, 4 Paenibacillus spp.
And 2 Serratia spp., Were obtained. According to the results of classical and
biochemical tests carried out with the aim of identifying bacterial isolates
All isolates were positive for the catalase test and for KOH tests, both
negative and positive results were obtained between the isolates. As a result
of the hypersensitivity tests (HR) performed on tobacco plants, it was
determined that the obtained isolates gave a negative result. As a result,
entomopathogens obtained from this study were diagnosed in the determined
target direction, It is thought that there are potentially usable species as
biopesticides in isolates, in particular against some damages of the
Curculionidae family which are harmful in leguminous forage plants.

Keywords

Biopesticides, Leguminous forage plants

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