The Effect of Peripheral Adropin Application on Hypothalamic Pituitary Adrenal Axis in Rats

Year 2019, Volume: 4 Issue: 2, 60 - 75, 28.10.2019

Mustafa Can Güler , Tuncer Nacar , Ersen Eraslan Ayhan Tanyeli , Elif Polat Selim Çomaklı

Abstract

Purpose:Hypothalamic pituitary adrenal (HPA) axis has many missions such as responses to stress and inflammatory factors. HPA axis is the primer stress response system. Adropin is a peptid structured hormone coded by energy homeostasis related gene. In this study biochemical, histopathologic and immunohistochemical effects of adropin hormone on HPA axis were examined.

Material and Method:Thirty two (32) Wistar Albinomale rats were used in the study. The rats were separated into 4 equal groups (n=8). The control group did not receive any applications; and the sham group was given adropin-dissolvent. Adropin was administered as intraperitoneal to the treatment groups at doses of 4 μg/kg and 40 μg/kg.The study lasted 10 days. On the 11^thday, the animals were sacrified, and relevant tissue samples were collected.

Findings:While cortisole, adrenaline, noradrenaline and serotonin levels decreased, contrary dopamin levels increased. There were no important changes on melatonin levels. As a consequence of immunohistochemical staining, CRH has shown increase in adropin groups compared to other groups.

Keywords

Adropin, HPA Axis, Hormone, Inflammatory, Stress

Supporting Institution

Department of Scientific Research Projects of Atatürk University

Project Number

2016/64

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