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Effect of Nitrogen Source on Growth and Protein and Lipid Amounts of a Freshwater Microalga Scenedesmus acutus

Year 2021, Volume: 16 Issue: 2, 215 - 220, 15.09.2021

Abstract

Bu çalışma, yeşil mikroalg Scenedesmus acutus’ un gelişim, protein ve lipit miktarı üzerinde azot kaynağının etkilerini incelemeyi amaçlamıştır. Bu amaçla Scenedesmus acutus’ un farklı azot kaynaklarına tepkileri standart besiyeri ortamındaki azot kaynakları yerine sadece KNO3, CaNO3, NaNO3, NH4NO3 ve (NH4)2SO4, ayrı ayrı ilave edilerek sıvı besi ortamlarında test edildi. Test edilen beş farklı nitrojen kaynağı arasında KNO3 ve CaNO3 en yüksek gelişimi sağladı. Protein miktarı azot kaynağı olarak KNO3 ve NH4NO3 kullanılan kültürlerde maksimum olurken lipit miktarı KNO3 ve NaNO3 kullanılan kültürlerde en yüksek miktarda tespit edildi.

Supporting Institution

Fırat Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi (FÜBAP)

Project Number

FUBAP, FF.18.01

Thanks

This research was funded by Firat University (Project No: FUBAB, FF.18.01). We would like to thank FUBAB for its financial support.

References

  • [1] Singh R, Parihar P, Singh M, Bajguz A, Kumar J, Singh S, Singh VP, Prasad SM. Uncovering potential applications of cyanobacteria and algal metabolites in biology, agricultureand medicine: current status and future prospects. Front Microbiol 2017; 8:515.
  • [2] Sharma J, Kumar SS, Sharma P, Gupta S, Malyan SK, Bishnoi NR. Effect of different nitrogen sources on growth of algal consortia. Annals of Agri-Bio Research. 2017; 22(2): 150-153.
  • [3] Vasileva IA, Ivanova JG, Gigova LG. Selection of nitrogen source affect the growth and metabolic enzyme activities of Chlorella vulgaris (Beijerinck) strain R-06/2 (Chlorophyta). Arch Biol Sci. 2020; 72(2): 291-300.
  • [4] Becker EW . Micro-algae as a source of protein. Biotechnology Advances. 2007; 25:207-210.
  • [5] Barka A, Blecker C. Microalgae as a potential source of single-cell proteins. A review. Biotechnol Agron Soc Environ.2016; 20(3): 427-436.
  • [6] Vasileva I, Marinova G, Gigova L. Effect of nitrogen source on the growth and biochemical composition of a new Bulgarian isolate of Scenedesmus sp. J BioSci. Biotechnol, 2015; SE/ONLINE: 125-129.
  • [7] Agirman N, Cetin AK. Effect of nitrogen limitation on growth, total lipid accumilation and protein amount in Scenedesmus acutus as biofuel reactor candidate. Natural Science and Discovery. 2017; 3(3): 33-38.
  • [8] Chen M, Tang H, Ma H, Holland TC, Ng KYS, Salley SO. Effect of nutrients on growth and lipid accumilation in the green algae Duinella tertiolecta. Bioresource Technology, 2011; 102:1649-1655.
  • [9] Ruangsomboon S. Effect of different media and nitrogen sources and levels on growth and lipid of green microalga Botryococcus braunii KMITL and its biodisel properties based on fatty acid composition. Bioresource Technology. 2015; 191: 377-384.
  • [10] Li T, Zheng Y, Yu L, Chen S. High productivity cultivation of a heat-resistant microalga Chlorella sorokinina for biofuel production. Bioresource Technology. 2013;13:60-67.
  • [11] Muthuraj M, Kumar V, Palabhanvi B, Das D. Evaluation of indigenous microalgal isolate Chlorella sp. FC IITC as a cell factory for biodisel production and scale up in outdoor conditions. J. Ind. Microbiol. Biotechnol. 2014; 41: 499-511.
  • [12] Norici A, Dalsass A, Giordano M. Role of phosphoenolpyruvate carboxylase in anaplerosis in the green microalga Dunaliella salina cultured under different nitrogen regimes. Physiol. Plant. 2002;116: 186-191.
  • [13] Wan MX, Wang RM, Xia JL, Rosenberg JN, Nie ZY, Kobayashi N, Oyler GA, Betenbaugh MJ. Physiological evaluation of a new Chlorella soronkininana isolated for its biomass production and lipid accumilation in photoautotrophic and heterotrophic cultures. Biotech. Bioeng. 2012; 109:1958-1964.
  • [14] Blight EG, Dyer WJ. A rapid method for total lipid extraction and prufication. Can J Biochem Physiol. 1959;37:911-917.
  • [15] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. The J Biol Chem. 1951;193:265-275.
  • [16] Gigova LG, Ivanova NJ. Microalgae respond differently to nitrogen availability during culturing. J. Biosci. 2015;40(2): 365-374.
  • [17] Yongmanitchai W, Ward OP. Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions. Appl Environ Microbiol. 1991; 57(2): 419–425.
  • [18] Hersh CM, Grumptain WG. Atrazine tolerance of algae isolated from two agracultural streams. Environ Toxicol Chem.1989; 8: 327-332.
  • [19] Molina GE, Sanchez PJA. EPA from Isochrysis galbana. Growth conditions and productivity. Process Biochem. 1992; 27: 299-305.
  • [20] Harwati TU, Willke T, Vorlop KD. Characterization of the lipid accumilation in a tropical freshwater microalgae Chlorococcum sp. Bioresource Technology 2012; 121, 54-60.
  • [21] Xu N, Zhang X, Fan X, Han L, Zeng C. Effect of nitrogen source and concentration on growth rate and fatty acid composition of Ellipsoidion sp. (Eustigmatophyta). Journal of Applied Phycology 2001; 13: 463-469.
  • [22] Sharma T, Gour RS, Kant A, Chauhan RS. Lipid content in Scenedesmus species correlates with multiple genes of fatty acid and triacylglycerol biosynthetic pathways. Algal Research 2015;12, 341-349.
  • [23] Kendirlioglu G, Cetin AK. Effect of nitrogen sources on growth, protein, total lipid amount and pigment content in Chlorella vulgaris. Fresenius Environmental Bulletin 2017; 28, 4A:3065-3072.
  • [24] Kendirlioglu Simsek G, Cetin AK.Effect of different wavelenghts of light on growth, pigment content and protein amount of Chlorella vulgaris. Fresenius Environmental Bulletin 2019; 26, 12A:7974-7980.
  • [25] Kumar DA, Gopal T, Harinath K, Sibi G. Responses in growth and lipid productivity of Chlorella vulgaris to different nitrogen sources. SOJ Microbiol InfectDis. 2017; 5(2): 1-6.
  • [26] Podevin M, De Francisci D, Holdt SL, Angelidaki I. Effect of nitrogen source and acclimatization on specific growth rates of microalgae determined by a high-throughput in vivo microplate autofluorescence method. J Appl Phycol 2015;27: 1415-1423.
  • [27] Gonzalez-Garcinuno A, Tabernero A, Sanchez-Alvarez JM, Martin del Valle. Effect of nitrogen source on growth and lipid accumilation in Scenedesmus abundans and Chlorella ellipsoidea. Bioresource Technology 2014;173: 334-341.
  • [28] Kim G, Mujtaba G, Lee K. Effects of nitrogen sources on cell growth and biochemical composition of marine chlorophyte Tetraselmis sp. for lipid production. Algae 2016; 31(3): 257-266.
Year 2021, Volume: 16 Issue: 2, 215 - 220, 15.09.2021

Abstract

Project Number

FUBAP, FF.18.01

References

  • [1] Singh R, Parihar P, Singh M, Bajguz A, Kumar J, Singh S, Singh VP, Prasad SM. Uncovering potential applications of cyanobacteria and algal metabolites in biology, agricultureand medicine: current status and future prospects. Front Microbiol 2017; 8:515.
  • [2] Sharma J, Kumar SS, Sharma P, Gupta S, Malyan SK, Bishnoi NR. Effect of different nitrogen sources on growth of algal consortia. Annals of Agri-Bio Research. 2017; 22(2): 150-153.
  • [3] Vasileva IA, Ivanova JG, Gigova LG. Selection of nitrogen source affect the growth and metabolic enzyme activities of Chlorella vulgaris (Beijerinck) strain R-06/2 (Chlorophyta). Arch Biol Sci. 2020; 72(2): 291-300.
  • [4] Becker EW . Micro-algae as a source of protein. Biotechnology Advances. 2007; 25:207-210.
  • [5] Barka A, Blecker C. Microalgae as a potential source of single-cell proteins. A review. Biotechnol Agron Soc Environ.2016; 20(3): 427-436.
  • [6] Vasileva I, Marinova G, Gigova L. Effect of nitrogen source on the growth and biochemical composition of a new Bulgarian isolate of Scenedesmus sp. J BioSci. Biotechnol, 2015; SE/ONLINE: 125-129.
  • [7] Agirman N, Cetin AK. Effect of nitrogen limitation on growth, total lipid accumilation and protein amount in Scenedesmus acutus as biofuel reactor candidate. Natural Science and Discovery. 2017; 3(3): 33-38.
  • [8] Chen M, Tang H, Ma H, Holland TC, Ng KYS, Salley SO. Effect of nutrients on growth and lipid accumilation in the green algae Duinella tertiolecta. Bioresource Technology, 2011; 102:1649-1655.
  • [9] Ruangsomboon S. Effect of different media and nitrogen sources and levels on growth and lipid of green microalga Botryococcus braunii KMITL and its biodisel properties based on fatty acid composition. Bioresource Technology. 2015; 191: 377-384.
  • [10] Li T, Zheng Y, Yu L, Chen S. High productivity cultivation of a heat-resistant microalga Chlorella sorokinina for biofuel production. Bioresource Technology. 2013;13:60-67.
  • [11] Muthuraj M, Kumar V, Palabhanvi B, Das D. Evaluation of indigenous microalgal isolate Chlorella sp. FC IITC as a cell factory for biodisel production and scale up in outdoor conditions. J. Ind. Microbiol. Biotechnol. 2014; 41: 499-511.
  • [12] Norici A, Dalsass A, Giordano M. Role of phosphoenolpyruvate carboxylase in anaplerosis in the green microalga Dunaliella salina cultured under different nitrogen regimes. Physiol. Plant. 2002;116: 186-191.
  • [13] Wan MX, Wang RM, Xia JL, Rosenberg JN, Nie ZY, Kobayashi N, Oyler GA, Betenbaugh MJ. Physiological evaluation of a new Chlorella soronkininana isolated for its biomass production and lipid accumilation in photoautotrophic and heterotrophic cultures. Biotech. Bioeng. 2012; 109:1958-1964.
  • [14] Blight EG, Dyer WJ. A rapid method for total lipid extraction and prufication. Can J Biochem Physiol. 1959;37:911-917.
  • [15] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. The J Biol Chem. 1951;193:265-275.
  • [16] Gigova LG, Ivanova NJ. Microalgae respond differently to nitrogen availability during culturing. J. Biosci. 2015;40(2): 365-374.
  • [17] Yongmanitchai W, Ward OP. Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions. Appl Environ Microbiol. 1991; 57(2): 419–425.
  • [18] Hersh CM, Grumptain WG. Atrazine tolerance of algae isolated from two agracultural streams. Environ Toxicol Chem.1989; 8: 327-332.
  • [19] Molina GE, Sanchez PJA. EPA from Isochrysis galbana. Growth conditions and productivity. Process Biochem. 1992; 27: 299-305.
  • [20] Harwati TU, Willke T, Vorlop KD. Characterization of the lipid accumilation in a tropical freshwater microalgae Chlorococcum sp. Bioresource Technology 2012; 121, 54-60.
  • [21] Xu N, Zhang X, Fan X, Han L, Zeng C. Effect of nitrogen source and concentration on growth rate and fatty acid composition of Ellipsoidion sp. (Eustigmatophyta). Journal of Applied Phycology 2001; 13: 463-469.
  • [22] Sharma T, Gour RS, Kant A, Chauhan RS. Lipid content in Scenedesmus species correlates with multiple genes of fatty acid and triacylglycerol biosynthetic pathways. Algal Research 2015;12, 341-349.
  • [23] Kendirlioglu G, Cetin AK. Effect of nitrogen sources on growth, protein, total lipid amount and pigment content in Chlorella vulgaris. Fresenius Environmental Bulletin 2017; 28, 4A:3065-3072.
  • [24] Kendirlioglu Simsek G, Cetin AK.Effect of different wavelenghts of light on growth, pigment content and protein amount of Chlorella vulgaris. Fresenius Environmental Bulletin 2019; 26, 12A:7974-7980.
  • [25] Kumar DA, Gopal T, Harinath K, Sibi G. Responses in growth and lipid productivity of Chlorella vulgaris to different nitrogen sources. SOJ Microbiol InfectDis. 2017; 5(2): 1-6.
  • [26] Podevin M, De Francisci D, Holdt SL, Angelidaki I. Effect of nitrogen source and acclimatization on specific growth rates of microalgae determined by a high-throughput in vivo microplate autofluorescence method. J Appl Phycol 2015;27: 1415-1423.
  • [27] Gonzalez-Garcinuno A, Tabernero A, Sanchez-Alvarez JM, Martin del Valle. Effect of nitrogen source on growth and lipid accumilation in Scenedesmus abundans and Chlorella ellipsoidea. Bioresource Technology 2014;173: 334-341.
  • [28] Kim G, Mujtaba G, Lee K. Effects of nitrogen sources on cell growth and biochemical composition of marine chlorophyte Tetraselmis sp. for lipid production. Algae 2016; 31(3): 257-266.
There are 28 citations in total.

Details

Primary Language English
Journal Section TJST
Authors

Ahmet Coban

Gökçe Kendirlioğlu Şimşek 0000-0001-8896-2893

Prof. Dr. A. Kadri Çetin 0000-0002-8687-2912

Project Number FUBAP, FF.18.01
Publication Date September 15, 2021
Submission Date February 26, 2021
Published in Issue Year 2021 Volume: 16 Issue: 2

Cite

APA Coban, A., Kendirlioğlu Şimşek, G., & Çetin, P. D. A. K. (2021). Effect of Nitrogen Source on Growth and Protein and Lipid Amounts of a Freshwater Microalga Scenedesmus acutus. Turkish Journal of Science and Technology, 16(2), 215-220.
AMA Coban A, Kendirlioğlu Şimşek G, Çetin PDAK. Effect of Nitrogen Source on Growth and Protein and Lipid Amounts of a Freshwater Microalga Scenedesmus acutus. TJST. September 2021;16(2):215-220.
Chicago Coban, Ahmet, Gökçe Kendirlioğlu Şimşek, and Prof. Dr. A. Kadri Çetin. “Effect of Nitrogen Source on Growth and Protein and Lipid Amounts of a Freshwater Microalga Scenedesmus Acutus”. Turkish Journal of Science and Technology 16, no. 2 (September 2021): 215-20.
EndNote Coban A, Kendirlioğlu Şimşek G, Çetin PDAK (September 1, 2021) Effect of Nitrogen Source on Growth and Protein and Lipid Amounts of a Freshwater Microalga Scenedesmus acutus. Turkish Journal of Science and Technology 16 2 215–220.
IEEE A. Coban, G. Kendirlioğlu Şimşek, and P. D. A. K. Çetin, “Effect of Nitrogen Source on Growth and Protein and Lipid Amounts of a Freshwater Microalga Scenedesmus acutus”, TJST, vol. 16, no. 2, pp. 215–220, 2021.
ISNAD Coban, Ahmet et al. “Effect of Nitrogen Source on Growth and Protein and Lipid Amounts of a Freshwater Microalga Scenedesmus Acutus”. Turkish Journal of Science and Technology 16/2 (September 2021), 215-220.
JAMA Coban A, Kendirlioğlu Şimşek G, Çetin PDAK. Effect of Nitrogen Source on Growth and Protein and Lipid Amounts of a Freshwater Microalga Scenedesmus acutus. TJST. 2021;16:215–220.
MLA Coban, Ahmet et al. “Effect of Nitrogen Source on Growth and Protein and Lipid Amounts of a Freshwater Microalga Scenedesmus Acutus”. Turkish Journal of Science and Technology, vol. 16, no. 2, 2021, pp. 215-20.
Vancouver Coban A, Kendirlioğlu Şimşek G, Çetin PDAK. Effect of Nitrogen Source on Growth and Protein and Lipid Amounts of a Freshwater Microalga Scenedesmus acutus. TJST. 2021;16(2):215-20.