Evaluation Of Oxidative Stress On Dogs With Demodicosis

Year 2023, , 7 - 13, 15.03.2023

Gözde Nur Sivel , Buğrahan Bekir Yağcı

https://doi.org/10.47748/tjvr.1119988

Abstract

Aim: In this study, it was aimed to investigate the effects of demodicosis on oxidative stress in dogs.
Materials and Methods: The material of the study is based on a total of 32 dogs, 21 of which are diagnosed with demodicosis and 11 of which are healthy, with different ages, genders and breeds. Examination for diagnostic evaluation of demodex is performed by examining the samples under the microscope which are taken by trichogram and deep skin scraping methods. In order to evaluate the oxidative stress on dogs with demodicosis at pre and post treatment groups and the control group without demodex diagnosis, the values of SOD (Superoxide dismutase), GPX (Glutation Peroxidase), GSH (Glutation) as antioxidant and MDA (Malondialdehyde) as oxidant are investigated.
Results ?: In clinical examinations, manifestations such as alopecia, erythema, generalized pruritus, hyperpigmentation, lichenification, pododermatitis, interdigital pruritus, lymphadenopathy are observed on dogs with demodicosis on pre and post treatment stages. In the analyzes performed in order to evaluate the oxidative stress, MDA 20.30 nmol/ml, GSH 4.9 nmol/ml, GPx 0.42 U/l, SOD 4.1 U/l are measured in dogs with clinical demodicosis. At the post treatment stage, the average values on the same dogs are measured as MDA 6.08 nmol/ml, GSH 8.11 nmol/ml, GPx 0.83 U/l, SOD 6.67 U/l, whilst in the control group they were measured as MDA 4.94 nmol/ml, GSH 9.73 nmol/ml, GPx 0.97 U/l, SOD 7.20 U/l. It is obtained that, GSH, GPx and SOD values in the control and post treatment groups are significantly higher (p˂0.001) and MDA value is lower (p˂0.001) than the clinical demodicosis group.
Conslusion: In dogs with clinical demodicosis, compared to control and post-treatment, higher level of MDA which is oxidant and lower level of GSA, GPx and SOD which are antioxidants showed that demodex is caused oxidative stress.

Keywords

Dermatitis, demodex, scabies, antioxidant, oxidative stress, oxidant

Project Number

Yok

References

• Abd Ellah, M. R. (2011). The Role of Liver Biopsy in Detection of Hepatic Oxidative Stress. SAGE-Hindawi Access to Research Veterinary Medicine International, 7 pages.
• Abdulaziz A.R. , Almuzaini . M. (2019, Mayıs). Evaluation of The Anti-Oxidative Activity and Trace Elements Concentrations in Demodex Canis Infected Dogs. Peer Res Nest., s. 1-5.
• Aslankoç R, D. D. (2019). The Role Of Antioxidant Enzymes İn Oxidative Stress - Superoxide Dismutase (Sod), Catalase (Cat) And Glutathione Peroxidase (3) 362 - 369,
• Aytuğ, N. (2012). Köpek ve Kedilerin İç Hastalıkları. TÜRKİYE: Medipress 487-492
• Beyazıt A., İ. T. (2010). Tek Tıp, Tek Sağlık Konseptine Katkı Demodicosisli Bir Köpek. Türkiye Parazitol Dergi, 34 (1): 68 71,
• Dimri, U., Ranjan, R., Kumar, N., Sharma, M.C., Swarup, D., Sharma, B. and Kataria, M. (2008) Changes in oxidative stress indices, zinc and copper concentrations in blood in canine demodicosis. Vet. Parasitol., 154(1-2): 98-102.
• Dündar A , Aslan, Y. D. (2000, ocak). HEKİMLİKTE OKSİDATİF STRES ve ANTİOKSİDANLAR Afyon Kocatepe Üniversitesi Yayınları , s. yayın no 29.
• Gökalp G, Kırbaş A. (2020). Köpek Demodikozisinde Genel Tanı ve Tedavi Yöntemleri. Bozok Vet Sci, 51-60.
• Hillier, A. and Desch C. E. (2002, march 1). Large-bodied Demodex mite infestation in 4 dogs. JAVMA, Vol 220, No. 5,.
• Hnilica, K. A., & Patterson, A. (2017). Küçük Hayvan Dermatoloji. Elsevier Inc. 135-145
• Küçük, A. (2021). Veteriner hekimliğinde viral hastalıkların Oksidatif stres üzerine etkisi effect of vıral dıseases On oxıdatıve stress ın veterınary medıcıne. 4th International Health Sciences and Life Congress, (s. 459- 466). Burdur.
• Mueller Ralf S., Rosenkrantz W., Bensignor E., Karas-Tecza J., Paterson T., Shipstone M.A. (2020). Diagnosis and treatment of demodicosis in dogs and cats. Ralf S. Mueller içinde, Veterinary Dermatology (s. 31: 4–e2 ).
• Pekmezci D., Pekmezci G.Z., Güzel M., Çenesiz S., Gürler A.T., Gökalp G. (2014). Efficacy of amitraz plus inactivated Parapoxvirus Ovis in the treatment of canine generalised demodicosis. Veterinary Record, 174: 556.
• Puppel K, Kapusta A & Kuczyńska B. (2015). The etiology of oxidative stress in the various species of animals, a review. Journal of the Science of Food and Agriculture, 95(11): 2179-2184.
• Russo, C., & Bracarense2, A. P. (2016, june). Oxidative stress in dogs. Semina: Ciências Agrárias, Londrina, v. 37, n. 3, , s. 1431-1440.
• Salem N, Y., Abdel-Saeed H . Farag H. S ,. Ghandour R. A (2020). Canine demodicosis: Hematological and biochemical alterations. Veterinary World,, 13(1): 68-72.
• Sgarbossa1*, R. S., Sechi1, G. V., Lucina, B. D., Paulo, M. R., Monti1, F. d., & Farias, M. R. (2017). The epidemiological and clinical aspects of Demodex injai demodicosis in dogs: a report of eight cases. Semina: Ciências Agrárias, 3387-3393.
• Şahin T., Şındak N, S. Y. (2004). Rottweiler Irkı Bir Köpekte Ekzema Olgusu . YYÜ Vet Fak Derg , 15 (1-2):79-82 .
• Tabakoğlu E., R. D. (2013). Veteriner Hekimlikte Oksidatif Stres ve Bazı Önemli Hastalıklarda Oksidatif Stresin Etkileri. AVKAE Derg, 69-75.
• Ural K, Erdoğan S. Gül G. (2019). Generalize demodikozisli köpeklerde deri pH’sı önemli bir biyofiziksel belirteç olabilir mi? MAE Vet Fak Derg, , 4 (2): 90-94.