Prevalence, molecular identification and determination of antibiotic susceptibility of methicillin-resistant Staphylococcus aureus in raw meat

Year 2024, , 29 - 33, 01.04.2024

Mukadderat Gökmen

https://doi.org/10.47748/tjvr.1381939

Abstract

Objective: The aim of this study was to determine the prevalence, molecular identification, and antibiotic susceptibility of MRSA in raw meats of retail sale in Balikesir, Turkey.
Materials and Methods: A total of 250raw meat samples (beef n=100, chicken n=100, and turkey n=50) were collected from various supermarkets. Mueller-Hinton Broth medium containing 6.5% NaCl was used for pre-enrichment and Baird Parker Agar (BPA) was used as a selective medium. Polymerase Chain Reaction technique was used to confirm the suspected colonies with the nuc gene for S. aureus and the mecA gene for MRSA. Kirby-Bauer standard disc diffusion method was applied for antibiotic susceptibility of MRSA.
Results: Of the 250 investigated raw meat samples, 21.2% were positive for S. aureus, which comprised 31% beef, 14% chicken, and 16 %turkey samples. Of the 250 investigated raw meat samples, 4% were positive for MRSA, which comprised 6% beef, 3% chicken, and 2% turkey samples. All MRSA isolates were found to be resistant to penicillin, sulfamethoxazole, trimethoprim, cefoxitin, and oxacillin, but they were susceptible to vancomycin.
Conclusion: In recent years, MRSA has been called a zoonotic pathogen that poses a serious risk for food safety and public health. Therefore, we believe that this study will shed light on new studies on the prevalence of MRSA in various animal-originated foods.

Keywords

Antibiotic susceptibility, MRSA, Prevalence, Raw Meats

Ethical Statement

Gerekli değil

Supporting Institution

Balıkesir Üniversitesi

Project Number

This study was supported by the Scientific Research Project Committee of Balıkesir University, Turkey, grant no: 2015/093.

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