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Year 2018, Volume: 2 Issue: 1, 19 - 25, 30.04.2018

Abstract

References

  • 1. Akgül A. Tıbbi Araştırmalarda İstatistiksel Analiz Teknikleri SPSS Uygulamaları, Yüksek Öğretim Kurulu Matbaası, Ankara, 1997.
  • 2. Akkuş İ. Serbest Radikaller ve Fizyopatolojik Etkileri, 1. Baskı, Mimoza Yayınları, Konya, 1995.
  • 3. Ashton T, Young IS, Peters JR, et al. Electron spin resonance spectroscopy, exercise, and oxidative stress: an ascorbic acid intervention study, J. Appl. Physiol.1999; 87, 6, 2032-2096.
  • 4. Aslan R, Şekeroğlu MR, Bayıroğlu F. Serbest radikal türlerin membran lipit peroksidasyonuna etkileri ve hücresel antioksidan savunma, Y.Y.Ü. Sağ. Bilim. Derg. 1995; 2, 137-142.
  • 5. Avellini L, Chiaradia E, Gaiti A. Effect of exercise training, selenium and vitamin E on some free radical scavengers in horses, Comp. Biochem. Physiol. B Biochem. Mol. Biol. 1 1999; 23, 2, 147-154.
  • 6. Beutler E, Duban O, Kelly BM. Improved method for the determination of blood glutathione, J. Lab. Clin. Med. 1963; 61, 882-888.
  • 7. Brady PS, Shelle JE, Ullrey DE. Rapid changes in equine erythrocyte glutathione reductase with exercise, A. J. of Vet. Res. 1977; 38, 7, 1045-1047.
  • 8. Byung PY. Cellular defenses againts damage from reactive species, Physiological Reviews 1994; 74, 1, 139-172.
  • 9. Chiaradia E, Avellini L, Rueca F, et al. Physical exercise, oxidative stres and muscle damage in race horses, Comp. Biochem. and Physiol. 1998; Part B, 119, 833-836.
  • 10. Clarkson PM. Antioxidants and Physicol Performance, Critical Reviews in Food Science and Nutrition 1995; 35, 1&2, 131-141.
  • 11. Demopoulus HB, Santomier JP, Seligman ML and Pietronigro DD. Free Radical Pathology: Rationale and Toxicology of Antioxidants and Other Supplements in Sports Medicine and Exercise Science, Sport, Health and Nutrition, Ed. Katch FI Human Kinetics Publishers 1986.
  • 12. Di Mascio P, Murphy ME, Sies H. Antioxidant Defense Systems: The role of caretenoids, tocopherols and thiols, Am. J. Clin. Nutr. 1991;53, 194-200.
  • 13. Duthie GC, Robertson JD, Maughan RJ, Morrice PC. Blood antioxidant status and erythrocyte lipid peroxidation following distance running. Arch. Biochem. Biophys. 1990; 282, 78-83.
  • 14. Dündar Y, Aslan R. Hekimlikte Oksidatif Stres ve Antioksidantlar, 1. Baskı, Afyon Kocatepe Üniversitesi Yayınları 2000, Afyon.
  • 15. Evans WJ. Vitamin E, vitamin C, and exercise, Am. J. Clin. Nutr. 2000;72, 2, 647-652.
  • 16. Evola CTA, Palmen NGM, Artur Y, Jannsen GME. Changes blood glutathione concentrations and in erythrocyte GR and GSH-S transferase activity after running training and after participitation in contests, Eur. J. Appl. Physiol 1992; 64, 354-362.
  • 17. Gutteridge JM. Lipid Peroxidation and antioxidans as biomarkers of tissue damage, Clin. Chem. 1995;41,12, 1819-1828.
  • 18. Holloszy JO. Biochemical adaptations in muscle, effects of exercise on mitochondrial oxygen uptake and respiratory enzyme activity in skeletal muscle, J. Biol. Chem. 1996;242, 9, 2278-2282.
  • 19. İsbir T. Antioxidant Sistemler In: Endotel, İzmir Tabip Odası Tıpta Temel Bilimler Kolu Sonbahar Okulu, Ekim 1994; 92-93, İzmir
  • 20. Jacob RA, Scala JH, Omaye ST. Biochemical incides of human vitamin C status, Am. J. Clin. Nutr. 1987;46, 818-826.
  • 21. Karlsson J. Antioxsidants and Exercise, Human Kinetics, Printed in United States of America 1997.
  • 22. Kovác G, Vrzgula L (1988). Vitamin E levels in the ovine serum after administration of vitamin E and selenium, The Amer. J. of Clin. Nutr. 1988; 48, 5, 1310-1315.
  • 23. Kumar CT, Reddy VK, Plasad M, Thyagataju K, Reddanna P. Dietary supplementation of vitamin E protects heart tissue from exercise induced oxidant stres, Med. Cell. Biochem. 1992;111, 109-115.
  • 24. Lang JK, Gohil K, Packer L, Burk RF. Selenium deficiency endurance exercise capasity, and antioxidant status in rats. J. Appl. Physiol. 1987;63, 2532-2535.
  • 25. Martinek RG. Method for determination of vitamin E history (total tocoferol) in serum, Clin Chem. 1964; 10,12, 1078-1086. “Alınmıştır” Oğan AC. Bazı Hayvan Türlerinin Kan Plazmas ında Vitamin E (Total Tokoferol)’nin Mevsimsel Değişiklikleri Üzerinde Çalışmalar, Uludağ Üniv. Sağlık Bilimleri Ens., Yüksek Lisans Tezi 1988, Bursa.
  • 26. Mills PC, Smith NC, Casas I, Haris P, Haris RC, Marlin DJ. Effect of exercise intensity and environmental stres on indices of oxidative stres and iron homeostasis during exercise in the horse, Eur. J. Appl. Physiol. 1996; 74, 60-66.
  • 27. Mills BJ, Richie JP, Lang CA. Sample processing alters glutathione and cysteine values in blood, Anal. Biochem. 1990;184, 263-267.
  • 28. Nemeth I, Boda D. Blood glutathione redox ratio as a parameter of oxidative stress in premature infants with IRDS, Free Radic. Biol. Med. 1994;16, 347-353.
  • 29. Ohno H, Sato Y, Yamashita K,et. al. The effect of brief physical exercise on free radical scavening enzyme systems in human red blood cell, Can. J. Pharmacol. 1986; 64, 1263-1269.
  • 30. Omaye ST, Turnbul JD, Sauberlich HE. Ascorbic acid analysis II. Determination after derivatisation with 2.2.dinitrophenylhidrazine selected methods for determination of ascorbic acid in animal cells, Tissues and Fluids. Meth. Enzymol.1979;62, 7-8.
  • 31. Ono K, Inui K, Hasegawa T, et. al. The changes of antioxidative enzyme activities in equine erythrocytes following exercise, Nippon Juigaku Zasshi Aug, 1990; 52, 4, 759-65.
  • 32. Poter NA, Chemistry of lipid peroxidation , Methods in Enzmol. 1984;105, 273-283.
  • 33. Rizzi R, Caroli A, Bolla P, Acciailoi A, Pagnacco G, Variability of reduced glutathione levels in Massese ewes and its effect on daily milk production, J. of Dairy Research 1988; 55, 345-353.
  • 34. Selçuk M. Sedanterler ile kuzey disiplini yapan antrene bireylerde programlı aerobik ve anaerobik egzersizlerin bazı antioksidan profiller üzerine etkilerinin araştırılması, Y.Y.Ü. Sağlık Bilimleri Enstitüsü Fizyoloji Anabilim Dalı Doktora Tezi 2003,Van.
  • 35. Siciliano PD, Parker AL, Lawrence LM. Effect of dietary vitamin E supplementation on the integrity of skeletal muscle in exercised horses, J. Anim. Sci.1997; 75, 1553-1560.
  • 36. Sushil JK, Mcuie R, Duett J, Herbst JJ. Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes, Diabetes 1989; 38, 1539- 1543.
  • 37. Suzuki I, Katoh N. A Simple and cheap methods for measuring serum vit. A in cattle using spectrophotometer, Jpn. J. Vet. Sci. 1990; 52, 6, 1281-1283.
  • 38. Sürmen Gür E, Öztürk E, Pündük Z, Tuncel P. Effect of vitamin E supplementation on post-exercise plasma lipid peroxidation and blood antioxidation status in smokers: with special reference to haemoconcentration effect, Eur. J. Appl. Physiol. 1999;79, 472-478.
  • 39. Şaşmaz GV. Genç erkek farelerde farklı sürelerdeki hafif egzersizin kas karaciğer antioksidan sistemlerine etkisi, Gazi Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı Uzmanlık Tezi 1997, Ankara
  • 40. Tıp S, Top Ş. E vitamininin kanser ve kardiovasküler hastalıklarla ilişkisi, T. Klin. Tıp Bil. Der. 1993;13, 288-289.
  • 41. Van-Der-Meulen JH, Mc Ardle A, Jackson MJ, Faulkner JA. Contraction-induced injury to the extensor digitorum longus muscles of rats: the role of vitamin E, J. Appl. Physiol. 1997;83, 3, 817-823.
  • 42. Vasankari TJ, Kujala UM, Rusko H, Sarna S, Ahotupa M. The effect of endurance exercise at moderate altitude on serum lipid peroxidation and antioxidative functions in humans, Eur. J. Appl. Physiol. 1997; 75, 397-399.
  • 43. White A, Estrada M, Walker K, et.al. Role of exercise and ascorbate on plasma antioxidant capacity in thoroughbred race horses. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2001;128, 1, 99- 104.
  • 44. Wilson R, Mansour MR, Stewart AD, Nimmo IA, Shepherd MJ, Riemersma RA. Lipid peroxidation in vivo is induced by exercise on a bicycle ergometer in athletes, Eur. J. Lipid Sci. Technol. 2001;103, 350-54.
  • 45. Yagi K. Lipid Peroxides and Related Padicals in Clinical Medicine, Free Radicals in Diagnostic Medicine, Ed. D. Armstrong, Plenum Press 1, 1994;7-27 New York.
  • 46. Yaşar S. Florozisli koyunlarda vitamin ve mineral düzeylerinin incelenmesi, Y.Y.Ü. Sağlık bilimleri Enstitüsü, Biyokimya anabilim Dalı Yüksek Lisans Tezi 2003,Van.

Investigation of the effect of vitamin E application on lipid peroxidation and antioxidants in exercised horses

Year 2018, Volume: 2 Issue: 1, 19 - 25, 30.04.2018

Abstract

Objective: In the this study, the effect of vitamin E plus
selenium application on malondialdehyde, glutathion, vitamin E, C, A and
b-karoten were aimed to investigate in exercised
horses.

Material and Methods: For this purpose, 50 healtly Anatolian type local horse breed aged
between 3-5 from Altındee Stud Farm were used. The animals were divided into
two equal groups. While 1
st group received nothing, horses in second
group were received vitamin E+selenium intramuscularly. Then animals in both
groups were exercised for 1500 meters. Blood samples were taken handly from all
animals before and after exercise. This samples were analysed for MDA, GSH,
vitamin E, C, A and β-caroten spectrophotometrically.

Results: MDA and GSH
concentration in 1
st group were found to increase significantly
(p<0.001) after exercise. On the other hand, serum vitamin E, C, A and
β-caroten levels did not changed significantly. In the second group, serum
vitamin E levels increased significantly (p<0.001) after vitamin E+selenium
application. Furthermore, MDA (p<0.05) and GSH (p<0.001) levels increased
significantly after exercise in the second group.Vitamin E levels decreased
significantly (p<0.01) after exercise. However, vitamin A and C levels did
not change significantly. In addition, in the second group, β-caroten levels
were also changed significantly (p<0.05) when the values obtained before
vitamin E application compared with the values obtained after vitamin E
application. When comparison made between groups, while MDA and vitamin E
values were statistically important (p<0.05), GSH, vitamin C, A and
β-caroten values were not important statistically







Conclusion: As a result, acute
exercise can increase free-radical production which, the results shows that
increase in both MDA and GSH can be shown as the indicator of  it. Furtermore, decrease in MDA level in
vitamin E applied group the indicator of the rise in antioxidan defence and
protective effect of vitamin E.

References

  • 1. Akgül A. Tıbbi Araştırmalarda İstatistiksel Analiz Teknikleri SPSS Uygulamaları, Yüksek Öğretim Kurulu Matbaası, Ankara, 1997.
  • 2. Akkuş İ. Serbest Radikaller ve Fizyopatolojik Etkileri, 1. Baskı, Mimoza Yayınları, Konya, 1995.
  • 3. Ashton T, Young IS, Peters JR, et al. Electron spin resonance spectroscopy, exercise, and oxidative stress: an ascorbic acid intervention study, J. Appl. Physiol.1999; 87, 6, 2032-2096.
  • 4. Aslan R, Şekeroğlu MR, Bayıroğlu F. Serbest radikal türlerin membran lipit peroksidasyonuna etkileri ve hücresel antioksidan savunma, Y.Y.Ü. Sağ. Bilim. Derg. 1995; 2, 137-142.
  • 5. Avellini L, Chiaradia E, Gaiti A. Effect of exercise training, selenium and vitamin E on some free radical scavengers in horses, Comp. Biochem. Physiol. B Biochem. Mol. Biol. 1 1999; 23, 2, 147-154.
  • 6. Beutler E, Duban O, Kelly BM. Improved method for the determination of blood glutathione, J. Lab. Clin. Med. 1963; 61, 882-888.
  • 7. Brady PS, Shelle JE, Ullrey DE. Rapid changes in equine erythrocyte glutathione reductase with exercise, A. J. of Vet. Res. 1977; 38, 7, 1045-1047.
  • 8. Byung PY. Cellular defenses againts damage from reactive species, Physiological Reviews 1994; 74, 1, 139-172.
  • 9. Chiaradia E, Avellini L, Rueca F, et al. Physical exercise, oxidative stres and muscle damage in race horses, Comp. Biochem. and Physiol. 1998; Part B, 119, 833-836.
  • 10. Clarkson PM. Antioxidants and Physicol Performance, Critical Reviews in Food Science and Nutrition 1995; 35, 1&2, 131-141.
  • 11. Demopoulus HB, Santomier JP, Seligman ML and Pietronigro DD. Free Radical Pathology: Rationale and Toxicology of Antioxidants and Other Supplements in Sports Medicine and Exercise Science, Sport, Health and Nutrition, Ed. Katch FI Human Kinetics Publishers 1986.
  • 12. Di Mascio P, Murphy ME, Sies H. Antioxidant Defense Systems: The role of caretenoids, tocopherols and thiols, Am. J. Clin. Nutr. 1991;53, 194-200.
  • 13. Duthie GC, Robertson JD, Maughan RJ, Morrice PC. Blood antioxidant status and erythrocyte lipid peroxidation following distance running. Arch. Biochem. Biophys. 1990; 282, 78-83.
  • 14. Dündar Y, Aslan R. Hekimlikte Oksidatif Stres ve Antioksidantlar, 1. Baskı, Afyon Kocatepe Üniversitesi Yayınları 2000, Afyon.
  • 15. Evans WJ. Vitamin E, vitamin C, and exercise, Am. J. Clin. Nutr. 2000;72, 2, 647-652.
  • 16. Evola CTA, Palmen NGM, Artur Y, Jannsen GME. Changes blood glutathione concentrations and in erythrocyte GR and GSH-S transferase activity after running training and after participitation in contests, Eur. J. Appl. Physiol 1992; 64, 354-362.
  • 17. Gutteridge JM. Lipid Peroxidation and antioxidans as biomarkers of tissue damage, Clin. Chem. 1995;41,12, 1819-1828.
  • 18. Holloszy JO. Biochemical adaptations in muscle, effects of exercise on mitochondrial oxygen uptake and respiratory enzyme activity in skeletal muscle, J. Biol. Chem. 1996;242, 9, 2278-2282.
  • 19. İsbir T. Antioxidant Sistemler In: Endotel, İzmir Tabip Odası Tıpta Temel Bilimler Kolu Sonbahar Okulu, Ekim 1994; 92-93, İzmir
  • 20. Jacob RA, Scala JH, Omaye ST. Biochemical incides of human vitamin C status, Am. J. Clin. Nutr. 1987;46, 818-826.
  • 21. Karlsson J. Antioxsidants and Exercise, Human Kinetics, Printed in United States of America 1997.
  • 22. Kovác G, Vrzgula L (1988). Vitamin E levels in the ovine serum after administration of vitamin E and selenium, The Amer. J. of Clin. Nutr. 1988; 48, 5, 1310-1315.
  • 23. Kumar CT, Reddy VK, Plasad M, Thyagataju K, Reddanna P. Dietary supplementation of vitamin E protects heart tissue from exercise induced oxidant stres, Med. Cell. Biochem. 1992;111, 109-115.
  • 24. Lang JK, Gohil K, Packer L, Burk RF. Selenium deficiency endurance exercise capasity, and antioxidant status in rats. J. Appl. Physiol. 1987;63, 2532-2535.
  • 25. Martinek RG. Method for determination of vitamin E history (total tocoferol) in serum, Clin Chem. 1964; 10,12, 1078-1086. “Alınmıştır” Oğan AC. Bazı Hayvan Türlerinin Kan Plazmas ında Vitamin E (Total Tokoferol)’nin Mevsimsel Değişiklikleri Üzerinde Çalışmalar, Uludağ Üniv. Sağlık Bilimleri Ens., Yüksek Lisans Tezi 1988, Bursa.
  • 26. Mills PC, Smith NC, Casas I, Haris P, Haris RC, Marlin DJ. Effect of exercise intensity and environmental stres on indices of oxidative stres and iron homeostasis during exercise in the horse, Eur. J. Appl. Physiol. 1996; 74, 60-66.
  • 27. Mills BJ, Richie JP, Lang CA. Sample processing alters glutathione and cysteine values in blood, Anal. Biochem. 1990;184, 263-267.
  • 28. Nemeth I, Boda D. Blood glutathione redox ratio as a parameter of oxidative stress in premature infants with IRDS, Free Radic. Biol. Med. 1994;16, 347-353.
  • 29. Ohno H, Sato Y, Yamashita K,et. al. The effect of brief physical exercise on free radical scavening enzyme systems in human red blood cell, Can. J. Pharmacol. 1986; 64, 1263-1269.
  • 30. Omaye ST, Turnbul JD, Sauberlich HE. Ascorbic acid analysis II. Determination after derivatisation with 2.2.dinitrophenylhidrazine selected methods for determination of ascorbic acid in animal cells, Tissues and Fluids. Meth. Enzymol.1979;62, 7-8.
  • 31. Ono K, Inui K, Hasegawa T, et. al. The changes of antioxidative enzyme activities in equine erythrocytes following exercise, Nippon Juigaku Zasshi Aug, 1990; 52, 4, 759-65.
  • 32. Poter NA, Chemistry of lipid peroxidation , Methods in Enzmol. 1984;105, 273-283.
  • 33. Rizzi R, Caroli A, Bolla P, Acciailoi A, Pagnacco G, Variability of reduced glutathione levels in Massese ewes and its effect on daily milk production, J. of Dairy Research 1988; 55, 345-353.
  • 34. Selçuk M. Sedanterler ile kuzey disiplini yapan antrene bireylerde programlı aerobik ve anaerobik egzersizlerin bazı antioksidan profiller üzerine etkilerinin araştırılması, Y.Y.Ü. Sağlık Bilimleri Enstitüsü Fizyoloji Anabilim Dalı Doktora Tezi 2003,Van.
  • 35. Siciliano PD, Parker AL, Lawrence LM. Effect of dietary vitamin E supplementation on the integrity of skeletal muscle in exercised horses, J. Anim. Sci.1997; 75, 1553-1560.
  • 36. Sushil JK, Mcuie R, Duett J, Herbst JJ. Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes, Diabetes 1989; 38, 1539- 1543.
  • 37. Suzuki I, Katoh N. A Simple and cheap methods for measuring serum vit. A in cattle using spectrophotometer, Jpn. J. Vet. Sci. 1990; 52, 6, 1281-1283.
  • 38. Sürmen Gür E, Öztürk E, Pündük Z, Tuncel P. Effect of vitamin E supplementation on post-exercise plasma lipid peroxidation and blood antioxidation status in smokers: with special reference to haemoconcentration effect, Eur. J. Appl. Physiol. 1999;79, 472-478.
  • 39. Şaşmaz GV. Genç erkek farelerde farklı sürelerdeki hafif egzersizin kas karaciğer antioksidan sistemlerine etkisi, Gazi Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı Uzmanlık Tezi 1997, Ankara
  • 40. Tıp S, Top Ş. E vitamininin kanser ve kardiovasküler hastalıklarla ilişkisi, T. Klin. Tıp Bil. Der. 1993;13, 288-289.
  • 41. Van-Der-Meulen JH, Mc Ardle A, Jackson MJ, Faulkner JA. Contraction-induced injury to the extensor digitorum longus muscles of rats: the role of vitamin E, J. Appl. Physiol. 1997;83, 3, 817-823.
  • 42. Vasankari TJ, Kujala UM, Rusko H, Sarna S, Ahotupa M. The effect of endurance exercise at moderate altitude on serum lipid peroxidation and antioxidative functions in humans, Eur. J. Appl. Physiol. 1997; 75, 397-399.
  • 43. White A, Estrada M, Walker K, et.al. Role of exercise and ascorbate on plasma antioxidant capacity in thoroughbred race horses. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2001;128, 1, 99- 104.
  • 44. Wilson R, Mansour MR, Stewart AD, Nimmo IA, Shepherd MJ, Riemersma RA. Lipid peroxidation in vivo is induced by exercise on a bicycle ergometer in athletes, Eur. J. Lipid Sci. Technol. 2001;103, 350-54.
  • 45. Yagi K. Lipid Peroxides and Related Padicals in Clinical Medicine, Free Radicals in Diagnostic Medicine, Ed. D. Armstrong, Plenum Press 1, 1994;7-27 New York.
  • 46. Yaşar S. Florozisli koyunlarda vitamin ve mineral düzeylerinin incelenmesi, Y.Y.Ü. Sağlık bilimleri Enstitüsü, Biyokimya anabilim Dalı Yüksek Lisans Tezi 2003,Van.
There are 46 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section 2018 Volume 2 Number 1
Authors

Neslihan Ormancı

Fatmagül Yur This is me

Publication Date April 30, 2018
Submission Date February 17, 2018
Published in Issue Year 2018 Volume: 2 Issue: 1

Cite

APA Ormancı, N., & Yur, F. (2018). Investigation of the effect of vitamin E application on lipid peroxidation and antioxidants in exercised horses. Turkish Journal of Veterinary Research, 2(1), 19-25.
AMA Ormancı N, Yur F. Investigation of the effect of vitamin E application on lipid peroxidation and antioxidants in exercised horses. TJVR. April 2018;2(1):19-25.
Chicago Ormancı, Neslihan, and Fatmagül Yur. “Investigation of the Effect of Vitamin E Application on Lipid Peroxidation and Antioxidants in Exercised Horses”. Turkish Journal of Veterinary Research 2, no. 1 (April 2018): 19-25.
EndNote Ormancı N, Yur F (April 1, 2018) Investigation of the effect of vitamin E application on lipid peroxidation and antioxidants in exercised horses. Turkish Journal of Veterinary Research 2 1 19–25.
IEEE N. Ormancı and F. Yur, “Investigation of the effect of vitamin E application on lipid peroxidation and antioxidants in exercised horses”, TJVR, vol. 2, no. 1, pp. 19–25, 2018.
ISNAD Ormancı, Neslihan - Yur, Fatmagül. “Investigation of the Effect of Vitamin E Application on Lipid Peroxidation and Antioxidants in Exercised Horses”. Turkish Journal of Veterinary Research 2/1 (April 2018), 19-25.
JAMA Ormancı N, Yur F. Investigation of the effect of vitamin E application on lipid peroxidation and antioxidants in exercised horses. TJVR. 2018;2:19–25.
MLA Ormancı, Neslihan and Fatmagül Yur. “Investigation of the Effect of Vitamin E Application on Lipid Peroxidation and Antioxidants in Exercised Horses”. Turkish Journal of Veterinary Research, vol. 2, no. 1, 2018, pp. 19-25.
Vancouver Ormancı N, Yur F. Investigation of the effect of vitamin E application on lipid peroxidation and antioxidants in exercised horses. TJVR. 2018;2(1):19-25.