Investigation of the effect of vitamin E application on lipid peroxidation and antioxidants in exercised horses

Year 2018, Volume: 2 Issue: 1, 19 - 25, 30.04.2018

Neslihan Ormancı , Fatmagül Yur

Abstract

Objective: In the this study, the effect of vitamin E plus
selenium application on malondialdehyde, glutathion, vitamin E, C, A and b-karoten were aimed to investigate in exercised
horses.

Material and Methods: For this purpose, 50 healtly Anatolian type local horse breed aged
between 3-5 from Altındee Stud Farm were used. The animals were divided into
two equal groups. While 1^st group received nothing, horses in second
group were received vitamin E+selenium intramuscularly. Then animals in both
groups were exercised for 1500 meters. Blood samples were taken handly from all
animals before and after exercise. This samples were analysed for MDA, GSH,
vitamin E, C, A and β-caroten spectrophotometrically.

Results: MDA and GSH
concentration in 1^st group were found to increase significantly
(p<0.001) after exercise. On the other hand, serum vitamin E, C, A and
β-caroten levels did not changed significantly. In the second group, serum
vitamin E levels increased significantly (p<0.001) after vitamin E+selenium
application. Furthermore, MDA (p<0.05) and GSH (p<0.001) levels increased
significantly after exercise in the second group.Vitamin E levels decreased
significantly (p<0.01) after exercise. However, vitamin A and C levels did
not change significantly. In addition, in the second group, β-caroten levels
were also changed significantly (p<0.05) when the values obtained before
vitamin E application compared with the values obtained after vitamin E
application. When comparison made between groups, while MDA and vitamin E
values were statistically important (p<0.05), GSH, vitamin C, A and
β-caroten values were not important statistically

Conclusion: As a result, acute
exercise can increase free-radical production which, the results shows that
increase in both MDA and GSH can be shown as the indicator of  it. Furtermore, decrease in MDA level in
vitamin E applied group the indicator of the rise in antioxidan defence and
protective effect of vitamin E.

Keywords

Antioxidant, horse, exercise, lipid peroxidation, vitamin E

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