Morphometric Analysis of Long Bones of Hamdani Crossbred Sheep Fetuses (Ovis aries) in the Second and Third Trimester: 3D Model

Year 2025, Volume: 9 Issue: 1, 1 - 6, 28.03.2025

Barış Can Güzel , Fatma İşbilir

https://doi.org/10.47748/tjvr.1513977

Abstract

Objective: In our study, it was aimed to obtain 3D models of femur and tibia bones using CT images in Hamdani crossbred sheep fetuses (second and third trimester) and to determine the developmental differences between sexes.
Material-Method: Fetuses of pregnant sheep slaughtered in Siirt province slaughterhouse were used. The gestational days of the fetuses were estimated using a previously determined formula. A total of 32 Hamdani crossbred sheep fetuses, 16 second trimester (8 females and 8 males) and 16 third trimester (8 females and 8 males) were used in the study. CT imaged the hind leg bones of the fetuses and the images were modeled in 3D Slicer software. Nine measurements were taken from the models. The measurements were evaluated statistically.
Result: In the second trimester, the FL parameter had a statistically significant difference between males and females (P<0.01). FDW parameter also showed a significant difference between males and females in this period (P<0.05). In the third trimester, the TPW measurement parameter had a highly significant difference between genders (P<0.01), and the ITTD parameter showed a significant difference between genders in the same period (P<0.05).
Conclusion: Osteometric studies are quite limited because bone development continues in the fetal period. In this sense, it is thought that the results of our research, which is the first 3D modeling study, will be useful in the fields of anatomy, zooarchaeology, taxonomy, obstetrics and gynecology, and surgery.

Keywords

Hamdani crossbred sheep, long bone, morphometry, 3D modeling.

Ethical Statement

This study was approved by the Siirt University Animal Experiments Local Ethics Committee (2024/05/22).

References

• Adapınar B. BT fiziği. In:Kaya T, ed. Temel Radyoloji Tekniği. Ankara: Nobel Tıp Kitabevi; 2016. pp.315-22.
• Akçasız ZN, Demircioğlu İ, Özkan E, Manuta N, Güzel BC. Three-dimensional pelvimetric evaluation of the pelvic cavity in different dog breeds. TJVR. 2024; 8:13-18.
• Aksoy A, Yavuz F. Çiftçilerin küçükbaş hayvan yetiştiriciliğini bırakma nedenlerinin analizi: doğu anadolu bölgesi örneği. ANAJAS. 2012; 27(2):76-79.
• Alpak H, Onar V, Mutuş R. The relationship between morphometric and long bone measurements of the Morkaraman sheep. Turk J Vet Anim Sci. 2009; 33(3):199-207.
• Bakici C, Akgun RO, Ekim O, et al. Three dimensional modeling and quantitative analysis of long bone parameters of rabbit using micro-computed tomography. IJVR. 2021; 22(2):140-145.
• Bosch U, Kasperczyk WJ. Healing of the patellar tendon autograft after posterior cruciate ligament reconstruction—-a process of ligamentization? An experimental study in a sheep model. AJSM. 1992; 20:558-566.
• Dağıstan E, Koç B, Gül A, Gül M. Koyunculuk üretim faaliyetinin faktör analizi: Orta ve Güney Anadolu Örneği. YYÜ Tar Bil Derg. 2008; 18(2):67-77.
• Dalga S. Applied anatomy to the Gurcu Goat´s mandible in Kafkas and its clinical significance in regional anesthesia. TJVR. 2021; 5:51-56.
• D'Urso PS, Barker TM, Earwaker WJ, et al. Stereolithographic biomodelling in cranio-maxillofacial surgery: a prospective trial. J Maxillofac Surg. 1999; 27(1):30-37.
• Eck K, Kunz E, Mendel C, Lühken G, Medugorac I. Morphometric measurements in lambs as a basis for future mapping studies. Small Rumin Res. 2019; 181:57-64.
• Evans PJ, Miniaci A, Hurtig MB. Manual punch versus power harvesting of osteochondral grafts. Arthroscopy. 2004; 20: -306-310.
• Field JR, Hearn TC, Costi JJ, et al. Ultimate tensile strength of a Leeds-Keio/autograft ACL reconstruction utilizing PLLA tibial staple fixation. Injury. 2003; 34:334-342.
• Guintard C, Lallemand M. Osteometric study of metapodial bones in sheep (Ovis aries, L. 1758). Ann Anat. 2003; 185:573-83.
• Guisasola I, Vaquero J, Forriol F. Knee immobilization on meniscal healing after suture: an experimental study in sheep. Clin Orthop. 2002; 395:227-233.
• Güzel BC, İşbilir F. Morphometric analysis of the skulls of a ram and ewe Romanov sheep (Ovis aries) with 3D modelling. Vet Med Sci. 2024; 10(2):e1396.
• Harmeyer J, Schlumbohm C. Pregnancy impairs ketone body disposal in late gestating ewes: implications for onset of pregnancy toxaemia. Research in veterinary science, 2006; 81(2):254–264. https://doi.org/10.1016/j.rvsc.2005.10.010
• İşbilir F, Güzel BC. Investigation of Metapodium and Acropodium Bones in Siirt-Colored Mohair Goat (Capra hircus) by 3D Modeling. Harran Üniv Vet Fak Derg. 2023; 12(2):245-252.
• Kartal M, Alpak H. Morphometric evaluation of the relationship between the distal femur and proximal tibia of the dogs. Animal Health Prod and Hyg. 2022; 11(2):66-71.
• Kim M, Huh KH, Yi WJ, Heo MS, Lee SS, Choi SC. Evaluation of accuracy of 3D reconstruction images using multi-detector CT and cone-beam CT. Imaging Sci Dent. 2012; 42:25-33.
• Marouf NO, Mimoune N, Benchabane A, Cedra W, Khelef D, Kaidi R. Comparative study between bone obtained by 3D printing and its original model. Veterinarska Stanica. 2024; 55(2):195-201.
• Mora G, Alvarez E, Ripalda P, Forriol F. Articular cartilage degeneration after frozen meniscus and Achilles tendon allograft transplantation: experimental study in sheep. Arthroscopy. 2003; 19:833-841.
• Noakes DE, Parkinson TJ, Gary C. Arthur's veterinary reproduction and obstetrics. 8th edn. Publishin country: Publisher; 2001. p. 68
• Örkiz M, Kaya F, Çalta H. Kangal Tipi Akkaraman Koyunlarının Bazı Önemli Verim Özellikleri. Lalahan Zoot Araşt Enst Derg. 1984; 24(1-4):15-33.
• Özkadif S, Haligür A. Morphometrical analysis of the Egyptian mongoose (Herpestes ichneumon) hind limb bones (pelvis, femur and crus) using threedimensional reconstructed images. Kafkas Univ Vet Fak Derg. 2022; 28(5):653-661.
• Özüdoğru Z, Özdemir D, Teke BE, Kırbaş M. A Study on Morphological and Morphometrical Parameters on the Skull of the Konya Merino Sheep. TJVR. 2023; 7 59-66.
• Pazvant G, Kahvecioglu KO. Studies on homotypic variation of forelimb and hindlimb long bones of rabbits. J Fac Vet Med Istanbul Univ. 2009; 35:23-29.
• Rowley-Conwy P. Improved separation of neolithic metapodials of sheep (Ovis) and goats (Capra) from Arene Candide Cave, Liguria, Italy. J Archaeol Sci. 1998; 25:251-258.
• Salami SO, Ibe CS, Umosen AD, Ajayi IE, Maidawa SM. Comparative Osteometric Study of Long Bones in Yankasa Sheep and Red Sokoto Goats. Int J Morphol. 2011; 29:100-104.
• Savio G, Baroni T, Concheri G, et al. Computation of femoral canine morphometric parameters in three-dimensional geometrical models. Vet Surg. 2016; 45:987-995.
• Selçuk M. Computed tomography reconstruction and morphometric analysis of the humerus and femur in New Zealand rabbits. Eurasian J Vet Sci. 2023; 39(4):164-170.
• Semerci A, Çelik AD. Türkiye’de küçükbaş hayvan yetiştiriciliğinin genel durumu. Mustafa Kemal Üniv Ziraat Fak Derg. 2016; 21(2):182-196.
• Singh LK, Singh U, Singh P. Fetal dystocia and estimation of fetal age in Sheep. IJAR. 2023; 44(2):104-106.
• Stull KE, Tise ML, Ali Z, Fowler DR. Accuracy and reliability of measurements obtained from computed tomography 3D volume rendered images. Forensic Sci Int. 2014; 238:133-140.
• Tamer B, Sarıözkan S. Yozgat Merkez İlçede Koyunculuk Yapan İşletmelerin Sosyo-Ekonomik Yapısı ve Üretim Maliyetleri. Erciyes Üniv Vet Fak Derg. 2017; 14(1):39-47.
• Turgut AO, Koca D, Ünver A. Comparison of blood ΒHBA measurement devices for diagnosis of subclinical pregnancy toxaemia in sheep: A field study. RDA. 2024; 59(5):e14589. https://doi.org/10.1111/rda.14589
• von Rechenberg B, Akens MK, Nadler D, et al. The use of photooxidized, mushroom-structured osteochondral grafts for cartilage resurfacing—a comparison to photooxidized cylindrical grafts in an experimental study in sheep. Osteoarthr Cartil. 2004; 12:201-216.
• Watson JPN. Fragmentation analysis of animal bone samples from archaeological sites. Archaeometry. 1972; 14:221-228.
• Weiler A, Peine R, Pashmineh-Azar A, Abel C, Sudkamp NP, Hoffmann RF. Tendon healing in a bone tunnel. Part I: Biomechanical results after biodegradable interference fit fixation in a model of anterior cruciate ligament reconstruction in sheep. Arthroscopy. 2002; 18:113-123.