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Year 2025, Volume: 9 Issue: 1, 75 - 88, 28.03.2025
https://doi.org/10.47748/tjvr.1570036

Abstract

References

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  • Abdualkader AM, Ghawi AM, Alaama M, et al. Leech therapeutic applications. Indian J Pharm Sci. 2013; 75:127-137.
  • Acıduman A. Sanizade Mehmet Ataullah Efendi ve Mi’yaru’l Etibba adlı eserinde çocuk hastalıkları. Cocuk Sagligi ve Hastalik. Derg. 2009;52(1):42-52.
  • Adams SL. The medicinal leech: A page from Annelids of internal medicine. Ann Int Med. 1988;109(5):399-405.
  • Alaama M, Kucuk O, Bilir B, et al. Development of Leech extract as a therapeutic agent: A chronological review. Pharmacol Res-Mod Chin Med. 2024; 10:100355.
  • Anonym (2024a) What can leeches be used for? Biopharm Leeches. Available at: https://www.biopharm-leeches.com/ what-can-leeches-be-used-for.html Accessed Sept 28, 2024.
  • Anonym (2024b). Ricarimpex Sangsues Médicinales. Available at: https://sangsue-medicinale.com/applications-medicales/ applications-en-medecine-veterinaire/. Accessed Sept 9, 2024.
  • Anonym. Hiruline corporation. Available at: https://hiruline.com/en/international_community/. Accessed Oct 9, 2024.
  • Aslan R. Holistic applications in veterinary medicine. In: Çoğun HY ed. New Approaches in Veterinary Physiology. Turkiye: BIDGE Publications; 2023. pp.37-94.
  • Ayhan H, Mollahaliloğlu S. Tıbbi sülük tedavisi: Hirudoterapi. Ankara Med J. 2018; 18(1):141-148.
  • Ayhan H, Özyurt Koçakoğlu N, Candan S. Functional morphology of the suckers and teeth of the medicinal leech Hirudo verbana Carena, 1820 (Annelida; Clitellata; Hirudinida): A scanning electron microscope study. Microsc Res Tech. 2021; 84(12):2930-2935.
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  • Ben-Yakir S. Feline thromboembolism (Saddle Thrombus) treated by medical leeches. In: Small Animal and Exotics. Proceedings of the North American Veterinary Conference, Orlando, Florida, USA. 2008b; 22:8.
  • Ben-Yakir S. Hemoacupuncture, or the therapy that sticks. In: Small Animal and Exotics. Proceedings of the North American Veterinary Conference, Orlando, Florida, USA, 2008a; 22:13-15.
  • Ben-Yakir S. Veterinary hirudotherapy (VHT) or therapy that sucks. Ganzheitliche Tiermedizin 2006; 20:95-100.
  • Bergsma M (2023). Little organic pharmacies: Leeches for horses. Available at: https://horsegrooms.com/little-organic-pharmacies-leeches-for-horses/. Accessed Sept 24, 2024.
  • Bichler J, Fritz H. Hirudin, a new therapeutic tool? Ann Hematol 1991; 63:67-76.
  • Blair WN. Notes on Hirudo medicinalis, the Medicinal Leech, as a British Species. In Proceedings of the Zoological Society of London. Oxford, UK: Blackwell Publishing Ltd; 1927. pp.999-1002.
  • Brennan ML. Complete holistic care and healing for horses. The owner's veterinary guide to alternative methods and remedies. Buckingham: Kenilworth Press; 2001.
  • Buote NJ. The use of medical leeches for venous congestion. Vet Comp Orthop Traumatol. 2014; 27(03):173-178.
  • Campos IT, Silva MM, Azzolini SS, Souza AF, Sampaio CA, Fritz H. Evaluation of phage display system and leech-derived tryptase inhibitor as a tool for understanding the serine proteinase specificities. Arch Biochem Biophys. 2004; 425:87-94.
  • Canpolat İ, Sağlam N. Treatment of aural hematomas in dogs with the medicinal leech, hirudo medicinalis. Fırat Üniversitesi Doğu Araştırmaları Dergisi. 2004; 2(2):67-69.
  • Caughey GH. Mast cell proteases as pharmacological targets. Eur J Pharmacol 2016; 778:44-55.
  • Cowley F. Treatment of hematoma of the canine ear. Vet Med Small Anim Clin. 1976; 71:283.
  • Das BK. An overview on hirudotherapy/leech therapy. Ind Res J Pharm Sci. 2014; 1:33-45.
  • Deckmyn H, Stassen JM, Vreys I, et al. Calin from Hirudo medicinalis, an inhibitor of platelet adhesion to collagen, prevents platelet-rich thrombosis in hamsters. Blood 1995; 85:712-719.
  • Dubielzig RR, Wilson JW, Seireg AA. Pathogenesis of canine aural hematomas. J Am Vet Med Assoc. 1984;185(8):873.
  • Dwivedi AP. Case study of leech application in diabetic foot ulcer. Int J Res Ayurveda Pharm. 2012; 3(5):748-751.
  • Elliott JM, Kutschera U. Medicinal Leeches: Historical Use, Ecology, Genetics, and Conservation. Freshw Rev. 2011; 4(1):21-41.
  • Erol ME. Investigation of the effects of medicinal leech and medicinal leech saliva extract in rats with flap model [Master Thesis]. Ankara: Gazi University; 2023.
  • Fernee R. (2011). Leeches and their effective use in veterinary medicine. Available at: https://www.vettimes.co.uk/article/ leeches-and-their-effective-use-in-veterinary-medicine/ Accessed Sept 29, 2024.
  • Fink E, Rehm H, Gippner C, et al. The primary structure of bdellin B-3 from the leech Hirudo medicinalis. Bdellin B-3 is a compact proteinase inhibitor of a “non-classical” Kazal type. It is present in the leech in a high molecular mass form. Biol Chem Hoppe Seyler. 1986; 367:1235-1242.
  • Franken C, Meijer CJ, Dijkman JH. Tissue distribution of antileukoprotease and lysozyme in humans. J Histochem Cytochem 1989; 37:493-498.
  • Gileva OS, Mumcuoglu KY, Hirudotherapy. Biotherapy-history, Principles and Practice: A Practical Guide to the Diagnosis and Treatment of Disease Using Living Organisms. Dordrecht: Springer Netherlands; 2013. pp.31-76.
  • Glöckner S. Eine retrospektive Studie über die Hufrehe bei Pferden: dargestellt an den Patienten der Klinik für Pferde, Allgemeine Chirurgie und Radiologie der Freien Universität Berlin aus den Jahren 1976–1995 [Doctoral thesis]. Berlin, Germany: Freie Universität Berlin; 2002.
  • Graf J. Symbiosis of Aeromonas veronii biovar sobria and Hirudo medicinalis, the medicinal leech: a novel model for digestive tract associations. Infect Immun. 1999; 67:1-7.
  • Gronwald W, Bomke J, Maurer T, et al. Structure of the leech protein saratin and characterization of its binding to collagen. J Mol Biol. 2008; 381:913-927.
  • Hallaji M, Parhamfar M, Raoufi E, Abtahi H. Cloning and high-level expression of the enzymatic region of phytase in E. coli. Int J Pept Res Ther 2019; 25(4):1431-1439.
  • Haq AU, Malik HU, Randhawa SS, Sudhan NA. Hirudotherapy in veterinary practice: A modern twist to ancient science. The Science World a Monthly e Magazine. 2021; 1(4):32-38.
  • Harsfalvi J, Stassen JM, Hoylaerts MF, et al. Calin from Hirudo medicinalis, an inhibitor of von Willebrand factor binding to collagen under static and flow conditions. Blood. 1995; 85:705-711.
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  • Hechtel FOP, Sawyer RT. Toward a taxonomic revision of the medicinal leech Hirudo medicinalis Linnaeus, 1758 (Hirudinea: Hirudinidae): re-description of Hirudo troctina Johnson, 1816 from North Africa. J Nat Hist. 2002; 36(11):1269-1289.
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Integrative Treatment Method in Veterinary Medicine: Hirudotherapy

Year 2025, Volume: 9 Issue: 1, 75 - 88, 28.03.2025
https://doi.org/10.47748/tjvr.1570036

Abstract

Traditional and complementary medicine methods have a long history in veterinary medicine. Medicinal leech therapy (hirudotherapy) is one of these methods. Medicinal leeches are sanguivorous invertebrates. While sucking blood to feed, they secrete saliva into the host's body. They contain over 100 bioactive molecules with analgesic, anti-inflammatory, anti-platelet, anticoagulant, and antimicrobial extracellular matrix degeneration effects. Consequently, the leeches treat the host by exhibiting a phlebotomic effect, a range of pharmacological effects due to bioactive molecules, and a reflex stimulation effect at the bite site. Recently, leeches have been used successfully in veterinary medicine to treat many diseases of animals, especially dogs, cats, and horses. The most common indications for the use of leeches are venous congestion, acute and chronic arthritis, laminitis in horses, diseases associated with inflammation of tendons, ligaments and fascia, vertebral diseases, and treatment of scars. Despite its frequent application in different countries, there needs to be more scientific literature on the veterinary use of leeches. This article aims to encourage further scientific investigation to expand the veterinary use of leech therapy, which is a cost-effective and relatively safe alternative to other methods, and to elucidate the potential effects of leech secretions.

References

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  • Abdualkader AM, Ghawi AM, Alaama M, et al. Leech therapeutic applications. Indian J Pharm Sci. 2013; 75:127-137.
  • Acıduman A. Sanizade Mehmet Ataullah Efendi ve Mi’yaru’l Etibba adlı eserinde çocuk hastalıkları. Cocuk Sagligi ve Hastalik. Derg. 2009;52(1):42-52.
  • Adams SL. The medicinal leech: A page from Annelids of internal medicine. Ann Int Med. 1988;109(5):399-405.
  • Alaama M, Kucuk O, Bilir B, et al. Development of Leech extract as a therapeutic agent: A chronological review. Pharmacol Res-Mod Chin Med. 2024; 10:100355.
  • Anonym (2024a) What can leeches be used for? Biopharm Leeches. Available at: https://www.biopharm-leeches.com/ what-can-leeches-be-used-for.html Accessed Sept 28, 2024.
  • Anonym (2024b). Ricarimpex Sangsues Médicinales. Available at: https://sangsue-medicinale.com/applications-medicales/ applications-en-medecine-veterinaire/. Accessed Sept 9, 2024.
  • Anonym. Hiruline corporation. Available at: https://hiruline.com/en/international_community/. Accessed Oct 9, 2024.
  • Aslan R. Holistic applications in veterinary medicine. In: Çoğun HY ed. New Approaches in Veterinary Physiology. Turkiye: BIDGE Publications; 2023. pp.37-94.
  • Ayhan H, Mollahaliloğlu S. Tıbbi sülük tedavisi: Hirudoterapi. Ankara Med J. 2018; 18(1):141-148.
  • Ayhan H, Özyurt Koçakoğlu N, Candan S. Functional morphology of the suckers and teeth of the medicinal leech Hirudo verbana Carena, 1820 (Annelida; Clitellata; Hirudinida): A scanning electron microscope study. Microsc Res Tech. 2021; 84(12):2930-2935.
  • Baskova I, Zavalova LL, Basanova AV, Sass AV. Separation of monomerizing and lysozyme activities of destabilase from medicinal leech salivary gland secretion. Biochem (Mosc). 2001; 66(12):1368-1373.
  • Baskova IP, Zavalova LL, Basanova AV, Moshkovskii SA, Zgoda VG. Protein profiling of the medicinal leech salivary gland secretion by proteomic analytical methods. Biochem (Mosc) 2004; 69:770 - 775.
  • Bayıroğlu ME. Investigation of the efficacy of leech therapy (hirudotherapy) in the acute muscle strain model on rats [Master Thesis]. Ankara: Ankara Yıldırım Beyazıt Uni̇versi̇ty; 2023.
  • Ben-Yakir S. Feline thromboembolism (Saddle Thrombus) treated by medical leeches. In: Small Animal and Exotics. Proceedings of the North American Veterinary Conference, Orlando, Florida, USA. 2008b; 22:8.
  • Ben-Yakir S. Hemoacupuncture, or the therapy that sticks. In: Small Animal and Exotics. Proceedings of the North American Veterinary Conference, Orlando, Florida, USA, 2008a; 22:13-15.
  • Ben-Yakir S. Veterinary hirudotherapy (VHT) or therapy that sucks. Ganzheitliche Tiermedizin 2006; 20:95-100.
  • Bergsma M (2023). Little organic pharmacies: Leeches for horses. Available at: https://horsegrooms.com/little-organic-pharmacies-leeches-for-horses/. Accessed Sept 24, 2024.
  • Bichler J, Fritz H. Hirudin, a new therapeutic tool? Ann Hematol 1991; 63:67-76.
  • Blair WN. Notes on Hirudo medicinalis, the Medicinal Leech, as a British Species. In Proceedings of the Zoological Society of London. Oxford, UK: Blackwell Publishing Ltd; 1927. pp.999-1002.
  • Brennan ML. Complete holistic care and healing for horses. The owner's veterinary guide to alternative methods and remedies. Buckingham: Kenilworth Press; 2001.
  • Buote NJ. The use of medical leeches for venous congestion. Vet Comp Orthop Traumatol. 2014; 27(03):173-178.
  • Campos IT, Silva MM, Azzolini SS, Souza AF, Sampaio CA, Fritz H. Evaluation of phage display system and leech-derived tryptase inhibitor as a tool for understanding the serine proteinase specificities. Arch Biochem Biophys. 2004; 425:87-94.
  • Canpolat İ, Sağlam N. Treatment of aural hematomas in dogs with the medicinal leech, hirudo medicinalis. Fırat Üniversitesi Doğu Araştırmaları Dergisi. 2004; 2(2):67-69.
  • Caughey GH. Mast cell proteases as pharmacological targets. Eur J Pharmacol 2016; 778:44-55.
  • Cowley F. Treatment of hematoma of the canine ear. Vet Med Small Anim Clin. 1976; 71:283.
  • Das BK. An overview on hirudotherapy/leech therapy. Ind Res J Pharm Sci. 2014; 1:33-45.
  • Deckmyn H, Stassen JM, Vreys I, et al. Calin from Hirudo medicinalis, an inhibitor of platelet adhesion to collagen, prevents platelet-rich thrombosis in hamsters. Blood 1995; 85:712-719.
  • Dubielzig RR, Wilson JW, Seireg AA. Pathogenesis of canine aural hematomas. J Am Vet Med Assoc. 1984;185(8):873.
  • Dwivedi AP. Case study of leech application in diabetic foot ulcer. Int J Res Ayurveda Pharm. 2012; 3(5):748-751.
  • Elliott JM, Kutschera U. Medicinal Leeches: Historical Use, Ecology, Genetics, and Conservation. Freshw Rev. 2011; 4(1):21-41.
  • Erol ME. Investigation of the effects of medicinal leech and medicinal leech saliva extract in rats with flap model [Master Thesis]. Ankara: Gazi University; 2023.
  • Fernee R. (2011). Leeches and their effective use in veterinary medicine. Available at: https://www.vettimes.co.uk/article/ leeches-and-their-effective-use-in-veterinary-medicine/ Accessed Sept 29, 2024.
  • Fink E, Rehm H, Gippner C, et al. The primary structure of bdellin B-3 from the leech Hirudo medicinalis. Bdellin B-3 is a compact proteinase inhibitor of a “non-classical” Kazal type. It is present in the leech in a high molecular mass form. Biol Chem Hoppe Seyler. 1986; 367:1235-1242.
  • Franken C, Meijer CJ, Dijkman JH. Tissue distribution of antileukoprotease and lysozyme in humans. J Histochem Cytochem 1989; 37:493-498.
  • Gileva OS, Mumcuoglu KY, Hirudotherapy. Biotherapy-history, Principles and Practice: A Practical Guide to the Diagnosis and Treatment of Disease Using Living Organisms. Dordrecht: Springer Netherlands; 2013. pp.31-76.
  • Glöckner S. Eine retrospektive Studie über die Hufrehe bei Pferden: dargestellt an den Patienten der Klinik für Pferde, Allgemeine Chirurgie und Radiologie der Freien Universität Berlin aus den Jahren 1976–1995 [Doctoral thesis]. Berlin, Germany: Freie Universität Berlin; 2002.
  • Graf J. Symbiosis of Aeromonas veronii biovar sobria and Hirudo medicinalis, the medicinal leech: a novel model for digestive tract associations. Infect Immun. 1999; 67:1-7.
  • Gronwald W, Bomke J, Maurer T, et al. Structure of the leech protein saratin and characterization of its binding to collagen. J Mol Biol. 2008; 381:913-927.
  • Hallaji M, Parhamfar M, Raoufi E, Abtahi H. Cloning and high-level expression of the enzymatic region of phytase in E. coli. Int J Pept Res Ther 2019; 25(4):1431-1439.
  • Haq AU, Malik HU, Randhawa SS, Sudhan NA. Hirudotherapy in veterinary practice: A modern twist to ancient science. The Science World a Monthly e Magazine. 2021; 1(4):32-38.
  • Harsfalvi J, Stassen JM, Hoylaerts MF, et al. Calin from Hirudo medicinalis, an inhibitor of von Willebrand factor binding to collagen under static and flow conditions. Blood. 1995; 85:705-711.
  • Haycox CL, Odland PB, Coltrera MD, Raugi GJ. Indications and complications of medicinal leech therapy. J Am Acad Dermatol. 1995; 33:1053-1055.
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There are 103 citations in total.

Details

Primary Language English
Subjects Veterinary Sciences (Other)
Journal Section 2025 Volume 9 Number 1
Authors

Fatma Çoruk 0009-0007-4523-9962

Hüseyin Ayhan 0000-0001-9519-5979

Early Pub Date March 26, 2025
Publication Date March 28, 2025
Submission Date October 19, 2024
Acceptance Date February 22, 2025
Published in Issue Year 2025 Volume: 9 Issue: 1

Cite

APA Çoruk, F., & Ayhan, H. (2025). Integrative Treatment Method in Veterinary Medicine: Hirudotherapy. Turkish Journal of Veterinary Research, 9(1), 75-88. https://doi.org/10.47748/tjvr.1570036
AMA Çoruk F, Ayhan H. Integrative Treatment Method in Veterinary Medicine: Hirudotherapy. TJVR. March 2025;9(1):75-88. doi:10.47748/tjvr.1570036
Chicago Çoruk, Fatma, and Hüseyin Ayhan. “Integrative Treatment Method in Veterinary Medicine: Hirudotherapy”. Turkish Journal of Veterinary Research 9, no. 1 (March 2025): 75-88. https://doi.org/10.47748/tjvr.1570036.
EndNote Çoruk F, Ayhan H (March 1, 2025) Integrative Treatment Method in Veterinary Medicine: Hirudotherapy. Turkish Journal of Veterinary Research 9 1 75–88.
IEEE F. Çoruk and H. Ayhan, “Integrative Treatment Method in Veterinary Medicine: Hirudotherapy”, TJVR, vol. 9, no. 1, pp. 75–88, 2025, doi: 10.47748/tjvr.1570036.
ISNAD Çoruk, Fatma - Ayhan, Hüseyin. “Integrative Treatment Method in Veterinary Medicine: Hirudotherapy”. Turkish Journal of Veterinary Research 9/1 (March 2025), 75-88. https://doi.org/10.47748/tjvr.1570036.
JAMA Çoruk F, Ayhan H. Integrative Treatment Method in Veterinary Medicine: Hirudotherapy. TJVR. 2025;9:75–88.
MLA Çoruk, Fatma and Hüseyin Ayhan. “Integrative Treatment Method in Veterinary Medicine: Hirudotherapy”. Turkish Journal of Veterinary Research, vol. 9, no. 1, 2025, pp. 75-88, doi:10.47748/tjvr.1570036.
Vancouver Çoruk F, Ayhan H. Integrative Treatment Method in Veterinary Medicine: Hirudotherapy. TJVR. 2025;9(1):75-88.