Influence of Fermentation Condition and Alkali Treatment on the Porosity and Thickness of Bacterial Cellulose Membranes

Abstract

The object of the present study was to produce bacterial cellulose (BC) membranes and studying the effect of carbon source, time and conditions of inoculation, type of alkali used for purification and the method of drying on its porosity and thickness of the resultant membranes. Acetobacter xylinum was isolated from local rotten juice, and used for membrane production. The highest porosity was attained when sucrose was used as a source of carbon compared to glucose, fructose and glycerol. However, fructose, glucose and glycerol resulted in higher pH value for the medium used as medium for bacterial growth. Using glycerol as the sole carbon source gave the highest bacterial cellulose and biomass (g/l) as compared to glucose, fructose and sucrose. Small inoculation led to high porosities and lowest thickness of the resultant membranes. Porosity of membranes was affected by the type of alkali used for the purification of the membranes. Application of K 2 CO 3 for purification gave the highest porosity while Na OH gave the lowest porosity. Hot air- drying of the membrane resulted in the lowest porosity as compared with freeze drying method which did not cause any damage to the porosity of the membrane

Keywords

• Bacterial cellulose membrane, Thickness and porosity, Acetobacter xylinum

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