Probiotic Potential of Lactic Acid Bacteria Isolated from Human Gut

Abstract

The aim of this study was to characterize human gut lactic acid bacteria group strains on the basis of their phenotypic profiles. In addition, their in vitro potential probiotic properties were evaluated with a view to identifying potential interesting application. Fifteen strains of lactic acid bacteria were isolated and identified. Among the strains, both biochemical and physiological characteristics differed noticeably and also showed a remarkable heterogeneity. The strains were grouped into the species Lactobacillus gasseri, Lb.casei ssp casei, Lb. delbrueckii ssp lactis, Lb. fermentum and Lb. delbrueckii ssp bulgaricus. These strains have an inhibitory activity against enterobacteria including Escherichia coli and Salmonella sp. A major part of these strains survived at pH 2.5 and in 0.3% bile salts. Additionally they produced no haemolysis, were resistant to kanamycin and adhered to epithelial cells. The results showed that the strains Lb. gasseri HG8 and Lb. fermentum HG3 have the best potential probiotic properties

Keywords

• Human gut, Lactic acid bacteria, Probiotic

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