Heavy Metal Scavenging Evaluation of in Vitro Grown Brassica Campestris Var. Sarsoon from the Tanneries Contaminated Soil Using Atomic Absorption Spectroscopy

Abstract

The study was conducted to evaluate and compare the scavenging efficiency of in-vivo and in-vitro grown Brassica campestris var. Sarsoon, for the uptake of Lead and Chromium( two major components of inorganic contaminants in the tanneries contaminated soils) using Atomic Absorption Spectroscopy. In vitro plants were grown on MS basal medium containing 2,4-D (2.5 mg/l ).Both invivo and hardened in-vitro grown plants were shifted to contaminated soil near Kasur Tanneries. After 6o days of growth in this contaminated soil, the plants bioassays were subjected to Atomic Absorption Spectroscopy for the estimation of lead and Chromium uptake. The in-vivo grown Brassica plants absorbed much lesser amount of both the metals(2.69 &1.62ppm) as compared to invitro grown plants which showed higher ranges(4.61 &2.69 ppm) of Pb and Cr respectively. The results supported our idea that, in future, the in-vitro grown hyper accumulator plants specially weeds can be used as an effective and better tool of phytoremediation ( compared to field grown ones) for the removal of heavy metals through their rhizosphere scavenging action, from the contaminated lands on a wider scale

Keywords

• Phytoremediation, In- vitro grown plants, Lead , Chromium, Inorganic, contaminants, Scavanging evaluation

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