Antiviral Activity of Polyphenols Extracts From Daucus carota against Herpes Simplex Virus type 1

Year 2013, Volume: 3 Issue: 1, 20 - 32, 23.07.2016

Zenab Aly Torky

Abstract

Herpes Simplex Virus type 1 (HSV-1), is a common and recurrent human virus with no medical cure. Polyphenols, on the other hand, are known to exhibit very strong oxygen scavenging properties; therefore they are considered a very rich source of antioxidants. In this research, the effect of extraction method using different solvents on the content of polyphenols extracted from the leaves and roots of D.carota was studied, and their relationship with antioxidant activity was investigated. The examined extracts were tested for cytotoxicity on Vero cell line, with reference to IC50, and other non-toxic concentrations of all the extracts. The antiviral activity against HSV-1 for all non-toxic concentrations of the extracts was determined using plaque reduction assay, which revealed that the inhibitory activity of the HSV-1 virus was dose dependent on the polyphenol content of the examined extracts. The MIC for all the extracts was also determined as well as the EC50 and the SI for all the extracts. Calculated SI values for the examined extracts showed promising SI on the aqueous extracts of the D.carota roots and leaves, and hence can be used as therapeutic medication for HSV-1. Direct contact between the HSV-1 and the examined extracts in cell-free assay system showed different degrees of virucidal activity depending on the polyphenolic content of these extracts. In order to study other possible mode of action, Vero cells were treated with the examined extracts before, during, and after virus infection to give an insight on the interference of the extract in each step in the virus life cycle. Examined extracts exhibited the antiviral activity against HSV-1 via blocking of the virus attachment and penetration and inhibition of the early stage of viral replications

Keywords

antiviral activity, HSV-1, polyphenols

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