AMYCOLATOPSİS ORİENTALİS BESİ ORTAMİNDA KARBON KAYNAĞİ OLAN GLİKOZ VE GLİSEROLÜN KONSANTRASYONUNA BAĞİMLİ VANKOMİSİN ÜRETİMİNİN DEĞİŞİMİ

Abstract

Değişen glikoz (5-20 g/L) ve gliserol (2.5-20 g/L) derişimlerde büyütülen A.orientalis in vankomisin üretim düzeyinin yanısıra ekstrasellüler glukoz, gliserol ve pH düzeyleri inkübasyon periyodu süresince incelenmiştir. A. orientalis büyüme hızı artan glikoz ve gliserol derişimleriyle artış gösterirken besi ortamının pH düzeylerinde anlamlı düzeyde azalışlar belirlenmiştir. Bununla birlikte, besi ortamındaki glikoz seviyeleri ile glikozun tüketim zamanı arasında pozitif korelasyon saptanmıştır. Benzer sonuç gliserol içeren ortamda da gözlenmiştir. Glikopeptid antibiyotiklerden biri olan vancomisinin A. orientalis de üretimi, besi ortamındaki glikozun 15 g/L ve gliserolün 10 g/L ye kadar artmasıyla anlamlı düzeyde artış göstermiştir. Karbon kaynağı olarak gliserolün yerine glikozun kullanılması, A. orientalis in vancomisin üretim düzeyinde anlamlı düzeyde artışa neden olmasına rağmen büyüme hızında etkili olmamıştır

Keywords

• A.orientalis, vankomisin , glikoz ve gliserol karbon kaynakları

VARIATIONS OF VANCOMYCIN PRODUCTION BY AMYCOLATOPSIS ORIENTALIS DEPENDING ON THE GLUCOSE AND GLYCEROL CONCENTRATIONS AS CARBON SOURCES

Abstract

Vancomycin production as well as extracellular glucose and glycerol level and pH level of A.orientalis growth in different glucose (5-20 g/L) and glycerol (2.5-20 g/L) concentrations of the medium was investigated during the incubation period. A. orientalis growth rate increased with the increases in glucose and glycerol concentration while pH levels of the growth medium decreased significantly. In addition, a positive correlation was determined between consumption time of extracellular glucose levels and glucose concentration in the culture medium. Similar results were also determined in glycerol supplemented medium. As a glycopeptid antibiotic, vancomycin production of A. orientalis increased with the increases in glucose and glycerol concentrations up to 15 and 10 g/L, respectively. In addition, substitution of glycerol with glucose significantly affected vancomycin antibiotic productions whereas the growth rate was close to that of the each other

Keywords

• A.orientalis, vancomycin, glucose and glycerol carbon sources

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