WHITE BLOOD CELL (WBC) COUNT OF DIFFERENT BAT (CHIROPTERA) SPECIES

Abstract

In this study, white blood cell counts and morphological characteristics of peripheral blood cells were determined. Bat specimens were collected from Koyunbaba Cave in Kırklareli Prov. in November 2007. The highest and lowest lymphocyte counts were determined in M. myotis (70.4±12.09) and R. ferrumequinum (53.3±14.83), respectively. When comparing the neutrophil counts of the species, it was seen that the highest value belongs to R. hipposideros (37.75±18.03) and the lowest one belongs to M. capaccinii (17.88±12.50). The highest percantage of monocytes was seen in R. ferrumequinum (12.55±5.77) where as R. hipposideros (7.75±3.89) has the lowest counts. The white blood corpuscle populations have also been compared with other bat studies

Keywords

• bats, granulocytes, agranulocytes, white blood cell count

Farklı Yarasa (Chiroptera) Türlerinin Beyaz Kan Hücresi Sayımı

Abstract

Bu çalışmada beyaz kan hücrelerinin sayısı ve periferik kan hücrelerinin morfolojik özellikleri saptanmıştır. Yarasa örnekleri, Koyunbaba Mağarası (Kırklareli)’ndan Kasım 2007’de toplanmıştır. En yüksek ve en düşük lenfosit sayıları sırasıyla M. myotis’te (70,4±12,09) ve R. ferrumequinum’da (53,3±14,83) belirlenmiştir. Türlerin nötrofil sayıları karşılaştırıldığında en yüksek değerin R. hipposideros’a (37,75±18,03), en düşük değerin ise M. capaccinii’ye (17,88±12,50) ait olduğu görülmüştür. En yüksek monosit oranı R. ferrumequinum’da (12,55±5,77) görülmüşken, R. hipposideros en düşük sayıya sahiptir (7,75±3,89). Beyaz kan hücresi sayıları ayrıca diğer yarasa çalışmaları ile karşılaştırıldı

Keywords

• yarasalar, granülositler, agranülositer, beyaz kan hücresi sayımı

References

1. ANAND KUMAR TC. Reproduction in the rat tailed bat Rhinopoma kinneari. J. Zool. Lond. 147: 147–155, 1965.
2. BADWAIK NK, RASWEILER JJ. Altered trophoblastic differentation and increased trophoblastic invasiveness during delayed development in the short-tailed fruit bat, Carollia perspicillata. Placenta. 22(1): 124–144, 2001.
3. DIETZ C, VON HELVERSEN O. Identification key to the bats of Europe – electronical publication, version 1.0, 72 pp.; available at www.uni-tuebingen.de/tierphys/Kontakt/mitarbeiter_seiten/dietz.htm., 2004.
4. ENCARNAÇAO J, DIETZ M, KIERDORF U. Reproductive condition and activity pattern of male Daubenton’s bats (Myotis daubentonii) in the summer habitat. Mamm. Biol. 69: 163–172, 2004.
5. GUYTON AC. Textbook of Medical Physiology. W.B. Saunders Company, Philadelphia, 1981. HELLGREN EC, VAUGHAN MR, KRIKPATRICK RL. Seasonal patterns in physiology and nutrition of black bears in Great Dismal Swamp, Virginia – North Carolina. Can. J. Zool. 67: 1837–1850, 1988.
6. HICKEY MBC, FENTON MB. Behavioural and thermoregulatory responses of female hoary bats, Lasiurus cinereus (Chiroptera: Vespertilionidae), to variations in prey availability. Ecoscience. 3: 414–422, 1996.
7. HILL JE, SMITH JD. Bats: A Natural History. Austin, TX: University of Texas Press. 243 p, 1984.
8. JONES K, PURVIS A, GITTLEMAN J. Biological correlates of extinction risk in bats. American Naturalist. 161: 601–614, 2003.

9. KOOPMAN HN, WESTGATE AJ, READ AJ, GASKIN DE. Blood chemistry of wild Harbor porpoises Phocoena phocoena (L.). Mar. Mamm. Sci. 11: 123–135, 1995.
10. LALL SB. Folliculogenesis in Rhinopoma kinneari Wroughton (Microchiroptera: Mammalia). Myotis. 23–24: 37–44, 1986.
11. MINEMATSU S, WATANABE M, TSUCHIYA N, WATANABE M, AMAGAYA S. Diurnal variations in blood chemical items in Sprague-Dawley rats. Exp. Anim. 44: 223–232, 1995.
12. PEHLIVANOĞLU B, BALKANCI ZD, RIDVANAGAOĞLU AY, DURMAZLAR N, ÖZTÜRK G, ERBAŞ D, OKUR H. Impact of stres, gender and menstrual cycle on immune system: possible role of nitric oxide. Arch. Physiol. Biochem. 109(4): 383–387, 2001.
13. RACEY PA, SWIFT SM. Feeding ecology of Pipistrellus pipistrellus (Chiroptera: Vespertilionidae) during pregnancy and lactation. I. Foraging behaviour. J. Anim. Ecol. 54: 205–215, 1985.
14. RACEY PA, TAM WH. Reproduction in male Pipistrellus pipistrellus (Mammalia: Chiroptera). J. Zool. 172: 101– 122, 1974.
15. RIEDESEL ML. Blood physiology. Pp.: 485–551. In: Wimsatt W. A. (ed.): Biology of Bats. Vol. III. Academic Pres. New York, 1977.
16. RYDELL J. Occurrence of bats in northernmost Sweden (650 N) and their feeding ecology in summer. J. Zool. 227: 517–529, 1992.
17. SILBERMAN DM, WALD M, GENARO AM. Effects of chronic mild stres on lymphocyte proliferative response. Participation of serum thyroid hormones and corticosterone. Int. Immunopharmacol. 2(4): 487–497, 2002.
18. SINGH UP, KRISHNA A. Seasonal changes in circulating testosterone and androstenedione concentration and their correlation with the anomalous reproductive pattern in the male Indian sheath-tailed bat, Taphozous longimanus. J. Exper. Zool. 287(1): 54–61, 2000.
19. TRIVEDI S. Seasonal histoenzymological and biochemical alterations in the ovary and uterus of certain chiropterans (Mammalia) of Rajasthan. Ph. D. thesis, M. L. Sukkadia University Udaipur, Rajastan, India, 1991.
20. TRIVEDI S, LALL SB. Histological and histochemical alterations in the ovary of nulliparous and parous bat Megaderma lyra lyra exhibiting absolute sinistral dominance of genitalia. Pp.:153-160. In: Hanak V., Horacek I. & Gaisler J. (eds.): European Bat Research 1987. Charles Univ. Press, Praha, 730 p, 1989.
21. TRIVEDI S, NARUKA K, SINGH P, DABI I, RATHORE S, RATHORE A. Differential leukocyte profile of Rhinopoma microphyllum kinneari. Vespertilio. 7: 169–176, 2003. VANDER AJ, SHERMAN JH, LUCIANO DS. Human Physiology. MacGraw-Hill Publication, Boston, 1994.
22. VAUGHAN TA, RYAN JM, CZAPLEWSKI NJ. Mammalogy, 4th Edition. Orlando, FL: Saunders College Publishing, 2000.
23. WESTHUYZEN VAN DER J. The diurnal cycle of some energy substrates in the fruit bat Rousettus aegyptiacus. S. Afr. J. Sci. 74: 99–101, 1978.
24. WIDMAIER EP, KUNZ TH. Basal, diurnal and stress-induced levels of glucose and glucocorticoids in captive bats. J. Exp. Zool. 265: 533–540, 1993.
25. WYK E, VAN DER BANK FH, VERDORN GH. Blood plasma calcium concentrations in captive and wild individuals of the Cape griffon vulture (Gyps coprotheres). Comp. Biochem. Physiol. A 104: 555–559, 1993