The effect of topical ascorbic acid application on the healing of rat tympanic membrane perforations

Year 2003, Volume: 11 Issue: 1, 1 - 4, 17.01.2003

Uğur Çınar Özgür Yiğit Berna Uslu Coşkun Seyhan Alkan Özlem Ünsal Gökhan Akgül Burhan Dadaş Tülay Başak

Abstract

Objectives: We investigated the effect of topical administration of ascorbic acid on the healing process of tympanic membrane perforations in rats.Design and Methods: Thermal myringotomy was induced in both ears of 22 albino rats. The right and left ears were assigned to topical applications of ascorbic acid and şaline solution, respectively. The same procedure was performed after 48 hours.Perforations were examined daily by otomicroscopy and healing periods were determined. For histopathologic examination, a single rat in which closure of the tympanic membranes was not com- pleted was sacrificed on days 5, 7, 10, and 12. Data were analyzed with the use of the Student’s t-test.Results: The mean durations of healing in the right and left ears were 7.6 and 8.3 days, respectively. No significant differences were found betvveen the applications of ascorbic acid and şaline solution with regard to the duration of closure and histopathologic healing criteria.Conclusion: Our data suggest that topical applica­ tion of ascorbic acid have no beneficial effect on the healing of rat tympanic membrane perforations.

Keywords

Administration, topical, ascorbic acid/therapeutic use, rats, tympanic membrane/pathology/drug effects, tympanic membrane perforation/pathology/drug effects, wound healing/drug effects

Topikal uygulanan askorbik asidin sıçan kulak zarı perforasyonlarında iyileşmeye etkisi

Abstract

Amaç: Sıçan kulak zarında oluflturulan perforasyonlarda topikal uygulanan askorbik asidin kulak zarınınkapanma süresi ve iyileflmesine etkisi arafltırıldı.Çalıflma Planı ve Yöntemler: Deneysel olarak oluflturulan termal miringotomi sonrasında 22 sıçanınsağ kulak zarına topikal askorbik asit, sol kulak zarına ise serum fizyolojik uygulandı. İfllem 48 saat sonra tekrarlandı. Sıçanların ameliyat mikroskobu ilegünlük otoskopik muayeneleri yapıldı ve perforasyonların kapanma süreleri belirlendi. Kulak zarlarının her ikisi de kapanmamıfl olan birer sıçanın yaflamı 5, 7, 10 ve 12. günlerde sonlandırıldı ve iyileflmehistopatolojik olarak değerlendirildi. Veriler Studentt-testi ile yorumlandı.Bulgu l a r : Askorbik asit grubunda kulak zarlarının ortalama kapanma süresi 7.6 gün, serum fizyolojik grubunda 8.3 gün bulundu. İki grup arasında kulak zarlarının kapanma süreleri ve histopatolojik iyileflme ölçütleri açısından anlamlı farklılık bulunmadı.Sonuç: Topikal olarak uygulanan askorbik asidin sıçan kulak zarındaki perforasyonun iyileflmesi üzerine etkili olmadığı sonucuna varıldı

Keywords

Uygulama, topikal, askorbik asit/terapötik kullanım, sıçan, timpanik membran/patoloji/ilaç etkisi, timpanik membran perforasyonu/patoloji/ilaç etkisi, yara iyileflmesi/ilaç etkisi

References

• Chauvin K, Bratton C, Parkins C. Healing large tym- panic membrane perforations using hyaluronic acid, basic fibroblast growth factor, and epidermal growth factor. Otolaryngol Head Neck Surg 1999;121:43-7.
• Uslu B, Seven H, Dadafl B, Baflak T, Çınar U, Özdoğan HC ve ark. Kobaylarda (guinea pig) topikal mitomisin C uygulamasının miringotomi üzerine etkisi. KBB Klinikleri 2001;3:135-42.
• Saika S, Uenoyama K, Hiroi K, Tanioka H, Takase K, Hikita M. Ascorbic acid phosphate ester and wound healing in rabbit corneal alkali burns: epithelial base- ment membrane and stroma. Graefes Arch Clin Exp Ophthalmol 1993;231:221-7.
• Vaxman F, Chalkiadakis G, Olender S, Maldonado H, Aprahamian M, Bruch JF, et al. Improvement in the healing of colonic anastomoses by vitamin B5 and C supplements. Experimental study in the rabbit. Ann Chir 1990;44:512-20. [Abstract]
• Kim M, Otsuka M, Yu R, Kurata T, Arakawa N. The distribution of ascorbic acid and dehydroascorbic acid during tissue regeneration in wounded dorsal skin of guinea pigs. Int J Vitam Nutr Res 1994;64:56-9.
• Collins CK, Lewis AE, Ringsdorf WM Jr, Cheraskin E. Effect of ascorbic acid on oral healing in guinea pigs. Int Z Vitaminforsch 1967;37:492-5.
• Spratley JE, Hellstrom SO, Mattsson CK, Pais-Clemente M. Topical ascorbic acid reduces myringosclerosis in perforated tympanic membranes. A study in the rat. Ann Otol Rhinol Laryngol 2001;11 0 : 5 8 5 - 9 1 .
• Yavuz E, Cınar U, Seven H, Vural C, Senvar A. The eff e c t of type 1 diabetes mellitus on the closing of rat tympan- ic membrane perforation. [Article in Turkish] Kulak B u run Bogaz Ihtis Derg 2001;8:458-62.
• Silverstein RJ, Landsman AS. The effects of a moderate and high dose of vitamin C on wound healing in a con- trolled guinea pig model. J Foot Ankle Surg 1999;38: 333-8.
• Kayaalp SO. Suda çözünen vitaminler. In: Rasyonel tedavi yönünden tıbbi farmakoloji. 9. baskı, Ankara: Hacettepe-Tafl Kitapçılık; 2000. s. 1564-75.
• Petroutsos G, Pouliquen Y. Effect of ascorbic acid on ulceration in alkali-burned corneas. Ophthalmic Res 1984;16:185-9.
• Ackerman Z, Seidenbaum M, Loewenthal E, Rubinow A. Overload of iron in the skin of patients with varicose ulcers. Possible contributing role of iron accumulation in pro g ression of the disease. Arch Dermatol 1988;124: 1 3 7 6 - 8 .
• Vaxman F, Olender S, Lambert A, Nisand G, Grenier JF. Can the wound healing process be improved by vit- amin supplementation? Experimental study on humans. Eur Surg Res 1996;28:306-14.
• Jackson TS, Xu A, Vita JA, Keaney JF Jr. Ascorbate pre- vents the interaction of superoxide and nitric oxide only at very high physiological concentrations. Circ Res 1998;83:916-22.
• Heller R, Munscher-Paulig F, Grabner R, Till U. L-Ascorbic acid potentiates nitric oxide synthesis in endothelial cells. J Biol Chem 1999;274:8254-60.
• L a u rent C, Hellstrom S, Fellenius E. Hyaluro n a n improves the healing of experimental tympanic mem- brane perforations. A comparison of preparations with different rheologic properties. Arch Otolaryngol Head Neck Surg 1988;114:1435-41.