Abstract
Objectives: We examined histopathologic and anatomical features of the guinea pig temporal bone and evaluated the dif-ferences and similarities with respect to the human ear.Study Design: Seventeen adult guinea pigs were deeply anesthetized with pentobarbital and then decapitated. Each temporal bone was removed and fixed in 10% formaldehyde for a week, then decalcified in 10% formic acid for three weeks. Paraffin-embedded specimens were serially and hor- izontally sectioned at 7-micron thickness. One of every five sections was stained with hematoxylin and eosin and studied under light microscopy.Results: The temporal bone consisted of a mastoid-like process, a tympanic bulla, a tympanic ring, a petrosal seg- ment, and a poorly developed squamosal bone. The tym panic bulla was a semispherical cavity surrounded by the tympanic ring. The head of the malleus and the body of the incus were fused, forming a malleoincudal complex. The diameters of the tympanic sulcus and the tympanic mem- brane were much greater than that of the tympanic ring, resulting in protrusion of the external ear-canal into the bulla. The Eustachian tube was J-shaped, lying in a bony hiatus at the anteromedial aspect of the bulla. The inner ear consisted of the cochlea, semicircular canals, and the vestibule. The cochlea made 3.5 tums and projected into the bulla. No internal auditory meatus was observed.Conclusion: The guinea pig temporal bone was found to have histological similarities to that of humans, making it a good model for selected experimental studies in otology.
Keywords
Ear/anatomy & histology ear middle/anatomy & histology guinea pigs/anatomy & histology temporal bone/anatomy & histology
References
- Asarch R, Abramson M, Litton WB. Surgical anatomy of the guinea pig ear. Ann Otol Rhinol Laryngol 1975; 84(2 Part 1):250-5.
- fiehitoğlu MA, Üneri C, Çelikoyar MM, Üneri A. Surgical anatomy of the guinea pig middle ear. Ear Nose Throat J 1990;69:91-7.
- Göksu N, Hazıroğlu R, Kemaloğlu Y, Karademir N, Bayramoğlu I, Akyıldız N. Anatomy of the guinea pig temporal bone. Ann Otol Rhinol Laryngol 1992;101: 699-704.
- Browning GC, Granich MS. Surgical anatomy of the temporal bone in the chinchilla. Ann Otol Rhinol Laryngol 1978;87:875-82.
- Sutbeyaz Y, Karasen M. Structural anatomy of the guinea pig temporal bone. [Article in Turkish] Kulak Burun Bogaz Ihtis Derg 1998:5;173-7.
- Schuknecht HF. Pathology of the ear. 2nd ed. Philadelphia: Lea and Febiger; 1993.
Abstract
Amaç: Bu çalıflmada guinea pig temporal kemiğinin histolojik anatomik özellikleri incelendi ve bunların insan kulağıile benzerlikleri ve farklılıkları değerlendirildi.Çalıflma Planı: Çalıflmaya 17 eriflkin guinea pig alındı.Derin anesteziden sonra hayvanların yaflamı sonlandırıldı.Çıkarılan temporal kemikler %10 formaldehidde bir haftasüreyle bekletildi; %10 formik asitte üç hafta süreyle dekalsifiye edildi. Parafine gömülen örneklerden seri olarakyatay planda 7 mikron kalınlığında kesitler alındı. Her beflkesitten biri hematoksilen-eosin ile boyandı ve ıflık mikroskobunda incelendi.Bulgular: Guinea pig temporal kemikleri mastoid benzeribir çıkıntı, timpanik bulla, timpanik halka, petröz parça veiyi geliflmemifl skuamöz kemikten oluflmaktaydı. Timpanikbulla, timpanik halka ile çevrili yarım daire fleklinde birboflluk olarak gözlendi. Malleus baflı ve inkus gövdesi kaynaflarak malleoinkudal kompleksi oluflturmaktaydı. Timpanik membran ve timpanik sulkusun çapı timpanik halkanınçapından büyüktü. Bu nedenle kemik dıfl kulak yolu kanalının bullaya doğru çıkıntı yaptığı görüldü. Östaki tübü Jfleklinde idi ve bullanın anteromedialinde bir kemik yarıkolarak gözlendi. İç kulak koklea, semisirküler kanallar vevestibülden oluflmaktaydı. Kokleanın 3.5 dönüfl yaptığı vebullaya çıkıntı oluflturduğu görüldü. İnternal akustik kanalgözlenmedi.Sonuç: Guinea pig temporal kemiği histolojik olarak insantemporal kemiğine benzer bulundu. Seçilmifl deneysel otoloji çalıflmalarında guinea pig kulağı iyi bir model oluflturabilir
Keywords
Kulak/anatomi ve histoloji kulak orta/anatomi ve histoloji guinea pig/anatomi ve histoloji temporal kemik/anatomi ve histoloji
References
- Asarch R, Abramson M, Litton WB. Surgical anatomy of the guinea pig ear. Ann Otol Rhinol Laryngol 1975; 84(2 Part 1):250-5.
- fiehitoğlu MA, Üneri C, Çelikoyar MM, Üneri A. Surgical anatomy of the guinea pig middle ear. Ear Nose Throat J 1990;69:91-7.
- Göksu N, Hazıroğlu R, Kemaloğlu Y, Karademir N, Bayramoğlu I, Akyıldız N. Anatomy of the guinea pig temporal bone. Ann Otol Rhinol Laryngol 1992;101: 699-704.
- Browning GC, Granich MS. Surgical anatomy of the temporal bone in the chinchilla. Ann Otol Rhinol Laryngol 1978;87:875-82.
- Sutbeyaz Y, Karasen M. Structural anatomy of the guinea pig temporal bone. [Article in Turkish] Kulak Burun Bogaz Ihtis Derg 1998:5;173-7.
- Schuknecht HF. Pathology of the ear. 2nd ed. Philadelphia: Lea and Febiger; 1993.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | April 10, 2003 |
| Published in Issue | Year 2003 Volume: 10 Issue: 2 |