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The effect of vitamin E on histopathologic healing and lipid peroxidation levels in experimentally induced traumatic tympanic membrane perforations

Year 2003, Volume: 10 Issue: 3, 87 - 92, 01.06.2003

Abstract

Objectives: To examine the favorable effects of vitamin E on tympanic membrane perforations induced mechanically in guinea pigs.Study Design: Bilateral tympanic membrane perforations of 1.8 mm were induced in 40 guinea pigs. The animals were ran- domly divided into two groups equal in number. One group remained untreated, while the othervvas administered vitamin E 100 mg/kg/day through intramuscular injections. On days 1,3, 5, and 7, five animals in each group were randomly sacrificed.Histopathologic changes in the tympanic membranes were evaluated and malondialdehyde levels were determined.Results: Significant increases were observed in epithelial thickness, fibroblastic proliferation, and neovascularization in the study group p<0.05 . Epithelial thickness was found to be increased in both groups beginning from the first day; however, this increase was more rapid in the study group. Although malondialdehyde levels showed significant increases on days 3 and 5 in both groups p<0.05 , they returned to the first day values in vitamin E-treated animals on day 7, vvhereas Con­ trols stili maintained high malondialdehyde levels.Conclusion: Vitamin E hastens the healing process of traumatic tympanic membrane perforations.

References

  • Spandow O, Hellstrom S. Animal model for persistent tympanic membrane perforations. Ann Otol Rhinol Laryngol 1993;102:467-72.
  • Ars B. Organogenesis of the middle ear structures. J Laryngol Otol 1989;103:16-21.
  • Gladstone HB, Jackler RK, Varav K. Tympanic mem- brane wound healing. An overview. Otolaryngol Clin North Am 1995;28:913-32.
  • L a u rent C, Hellstrom S, Fellenius E. Hyaluro n a n improves the healing of experimental tympanic mem- brane perforations. A comparison of preparations with different rheologic properties. Arch Otolaryngol Head Neck Surg 1988;114:1435-41.
  • Stenfors LE. Repair of tympanic membrane perfora- tions using hyaluronic acid: an alternative to myringo- plasty. J Laryngol Otol 1989;103:39-40.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid perox- ides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
  • Manning SC, Casselbrant M, Lammers D. Otolaryngologic manifestations of child abuse. Int J Pediatr Otorhinolaryngol 1990;20:7-16.
  • Matt BH, Miller RP, Meyers RM, Campbell JM, Cotton RT. Incidence of perforation with Goode T-tube. Int J Pediatr Otorhinolaryngol 1991;21:1-6.
  • Rybak LP, Johnson DW. Tympanic membrane perfora- tions from water sports: treatment and outcome. Otolaryngol Head Neck Surg 1983;91:659-62.
  • Austin DF. Chronic otitis media. In: Ballenger JJ, Snow JB, editors. Otolaryngology head and neck surgery. 15th ed. Baltimore, MD: Williams & Wilkins; 1996. p. 1010-37.
  • Shambough GE, Glasscock ME. Closure of tympanic membrane perforations. In: Shambaugh GE, Glasscock ME, editors. Surgery of the ear. 4th ed. Philadelphia: W.B. Saunders; 1990. p. 334-49.
  • Machlin LJ, Bendich A. Free radical tissue damage: p rotective role of antioxidant nutrients. FASEB J 1987;1:441-5.
  • Kawana M, Kawana C, Yokoo T, Quie PG, Giebink GS. Oxidative metabolic products released from polymor- phonuclear leukocytes in middle ear fluid during experimental pneumococcal otitis media. Infect Immun 1991;59:4084-8.
  • Parks RR, Huang CC, Haddad J Jr. Evidence of oxygen radical injury in experimental otitis media. Laryngoscope 1994;104(11 Pt 1):1389-92.
  • Igarashi A, Uzuka M, Nakajima K. The effects of vitamin E deficiency on rat skin. Br J Dermatol 1989;121:43-9.
  • Ehrlich HP, Tarver H, Hunt TK. Inhibitory effects of vitamin E on collagen synthesis and wound repair. Ann Surg 1972;175:235-40.
  • Haberal M, Hamaloğlu E, Bora S, Öner G, Bilgin N. The effects of vitamin E on immune regulation after thermal injury. Burns Incl Therm Inj 1988;14:388-93.
  • Samoilov AV, Seifulla RD, Shekhter AB, Volozhin AI, Denisov AB, Druzhinina RA, et al. The characteristics of the wound-healing action of solubilized alpha-toco- p h e rol acetate. Eksp Klin Farmakol 1993;56:59-62. [Abstract]

Travmatik timpanik membran perforasyonlarında E vitamininin histopatolojik iyileşme ve lipid peroksidasyon düzeyleri üzerine etkileri: Deneysel çalışma

Year 2003, Volume: 10 Issue: 3, 87 - 92, 01.06.2003

Abstract

Amaç: E vitamininin, mekanik etkiyle oluflturulan timpanikmembran perforasyonlarında iyileflme üzerine olası olumlu etkilerini ortaya koymak. Çal ı flma Planı: Kırk adet guinea pig’in her iki timpanik membranında, standart 1.8 mm’lik perforasyon oluflturuldu. Denekler 20’flerli iki gruba ayrıldı. Bir gruba tedavi uygulanmazken,diğer gruba 100 mg/kg/gün E vitamini intramusküler enjeksiyonla verildi. Perforasyon oluflturulduktan sonra, 1, 3, 5 ve 7.günlerde gruplardan rastgele seçilen befl kobayın yaflamısonlandırıldı. Timpanik membrandaki histopatolojik değifliklikler değerlendirildi ve doku malondialdehid düzeyleri ölçüldü. Bulgular: Epitel kalınlığı, fibroblast proliferasyonu ve neovaskülarizasyon çalıflma grubunda anlamlı derecedefarklı bulundu p

References

  • Spandow O, Hellstrom S. Animal model for persistent tympanic membrane perforations. Ann Otol Rhinol Laryngol 1993;102:467-72.
  • Ars B. Organogenesis of the middle ear structures. J Laryngol Otol 1989;103:16-21.
  • Gladstone HB, Jackler RK, Varav K. Tympanic mem- brane wound healing. An overview. Otolaryngol Clin North Am 1995;28:913-32.
  • L a u rent C, Hellstrom S, Fellenius E. Hyaluro n a n improves the healing of experimental tympanic mem- brane perforations. A comparison of preparations with different rheologic properties. Arch Otolaryngol Head Neck Surg 1988;114:1435-41.
  • Stenfors LE. Repair of tympanic membrane perfora- tions using hyaluronic acid: an alternative to myringo- plasty. J Laryngol Otol 1989;103:39-40.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid perox- ides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
  • Manning SC, Casselbrant M, Lammers D. Otolaryngologic manifestations of child abuse. Int J Pediatr Otorhinolaryngol 1990;20:7-16.
  • Matt BH, Miller RP, Meyers RM, Campbell JM, Cotton RT. Incidence of perforation with Goode T-tube. Int J Pediatr Otorhinolaryngol 1991;21:1-6.
  • Rybak LP, Johnson DW. Tympanic membrane perfora- tions from water sports: treatment and outcome. Otolaryngol Head Neck Surg 1983;91:659-62.
  • Austin DF. Chronic otitis media. In: Ballenger JJ, Snow JB, editors. Otolaryngology head and neck surgery. 15th ed. Baltimore, MD: Williams & Wilkins; 1996. p. 1010-37.
  • Shambough GE, Glasscock ME. Closure of tympanic membrane perforations. In: Shambaugh GE, Glasscock ME, editors. Surgery of the ear. 4th ed. Philadelphia: W.B. Saunders; 1990. p. 334-49.
  • Machlin LJ, Bendich A. Free radical tissue damage: p rotective role of antioxidant nutrients. FASEB J 1987;1:441-5.
  • Kawana M, Kawana C, Yokoo T, Quie PG, Giebink GS. Oxidative metabolic products released from polymor- phonuclear leukocytes in middle ear fluid during experimental pneumococcal otitis media. Infect Immun 1991;59:4084-8.
  • Parks RR, Huang CC, Haddad J Jr. Evidence of oxygen radical injury in experimental otitis media. Laryngoscope 1994;104(11 Pt 1):1389-92.
  • Igarashi A, Uzuka M, Nakajima K. The effects of vitamin E deficiency on rat skin. Br J Dermatol 1989;121:43-9.
  • Ehrlich HP, Tarver H, Hunt TK. Inhibitory effects of vitamin E on collagen synthesis and wound repair. Ann Surg 1972;175:235-40.
  • Haberal M, Hamaloğlu E, Bora S, Öner G, Bilgin N. The effects of vitamin E on immune regulation after thermal injury. Burns Incl Therm Inj 1988;14:388-93.
  • Samoilov AV, Seifulla RD, Shekhter AB, Volozhin AI, Denisov AB, Druzhinina RA, et al. The characteristics of the wound-healing action of solubilized alpha-toco- p h e rol acetate. Eksp Klin Farmakol 1993;56:59-62. [Abstract]
There are 18 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Nihat Susaman This is me

Şinasi Yalçın This is me

İrfan Kaygusuz This is me

Turgut Karlıdağ This is me

Üzeyir Gök This is me

Nevin İlhan This is me

İbrahim Hanifi Özercan This is me

Publication Date June 1, 2003
Published in Issue Year 2003 Volume: 10 Issue: 3

Cite

APA Susaman, N., Yalçın, Ş., Kaygusuz, İ., Karlıdağ, T., et al. (2003). Travmatik timpanik membran perforasyonlarında E vitamininin histopatolojik iyileşme ve lipid peroksidasyon düzeyleri üzerine etkileri: Deneysel çalışma. The Turkish Journal of Ear Nose and Throat, 10(3), 87-92.
AMA Susaman N, Yalçın Ş, Kaygusuz İ, Karlıdağ T, Gök Ü, İlhan N, Özercan İH. Travmatik timpanik membran perforasyonlarında E vitamininin histopatolojik iyileşme ve lipid peroksidasyon düzeyleri üzerine etkileri: Deneysel çalışma. Tr-ENT. June 2003;10(3):87-92.
Chicago Susaman, Nihat, Şinasi Yalçın, İrfan Kaygusuz, Turgut Karlıdağ, Üzeyir Gök, Nevin İlhan, and İbrahim Hanifi Özercan. “Travmatik Timpanik Membran perforasyonlarında E Vitamininin Histopatolojik iyileşme Ve Lipid Peroksidasyon düzeyleri üzerine Etkileri: Deneysel çalışma”. The Turkish Journal of Ear Nose and Throat 10, no. 3 (June 2003): 87-92.
EndNote Susaman N, Yalçın Ş, Kaygusuz İ, Karlıdağ T, Gök Ü, İlhan N, Özercan İH (June 1, 2003) Travmatik timpanik membran perforasyonlarında E vitamininin histopatolojik iyileşme ve lipid peroksidasyon düzeyleri üzerine etkileri: Deneysel çalışma. The Turkish Journal of Ear Nose and Throat 10 3 87–92.
IEEE N. Susaman, “Travmatik timpanik membran perforasyonlarında E vitamininin histopatolojik iyileşme ve lipid peroksidasyon düzeyleri üzerine etkileri: Deneysel çalışma”, Tr-ENT, vol. 10, no. 3, pp. 87–92, 2003.
ISNAD Susaman, Nihat et al. “Travmatik Timpanik Membran perforasyonlarında E Vitamininin Histopatolojik iyileşme Ve Lipid Peroksidasyon düzeyleri üzerine Etkileri: Deneysel çalışma”. The Turkish Journal of Ear Nose and Throat 10/3 (June 2003), 87-92.
JAMA Susaman N, Yalçın Ş, Kaygusuz İ, Karlıdağ T, Gök Ü, İlhan N, Özercan İH. Travmatik timpanik membran perforasyonlarında E vitamininin histopatolojik iyileşme ve lipid peroksidasyon düzeyleri üzerine etkileri: Deneysel çalışma. Tr-ENT. 2003;10:87–92.
MLA Susaman, Nihat et al. “Travmatik Timpanik Membran perforasyonlarında E Vitamininin Histopatolojik iyileşme Ve Lipid Peroksidasyon düzeyleri üzerine Etkileri: Deneysel çalışma”. The Turkish Journal of Ear Nose and Throat, vol. 10, no. 3, 2003, pp. 87-92.
Vancouver Susaman N, Yalçın Ş, Kaygusuz İ, Karlıdağ T, Gök Ü, İlhan N, Özercan İH. Travmatik timpanik membran perforasyonlarında E vitamininin histopatolojik iyileşme ve lipid peroksidasyon düzeyleri üzerine etkileri: Deneysel çalışma. Tr-ENT. 2003;10(3):87-92.