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A general review on the procedures in a human temporal bone laboratory

Year 2006, Volume: 16 Issue: 5, 235 - 240, 14.10.2006

Abstract

Structures of the human ear are usually inaccessible during life for examination of pathologic changes of the underlying disease, which is only possible with post­ mortem studies of the human temporal bone. Hence, human temporal bone laboratories serve as a unique source of material for research in this respect. They enable comparison between histologic findings of tem­ poral bone sections and the ear pathologies docu- mented prior to death, as well as comparison of diseased ears with any selected temporal bone specimens, both of which provide invaluable knowledge to be shared among researchers and other laboratories.This article aims to provide insight into the functions of temporal bone laboratories and to familiarize the reader with histopathologic studies conducted therein.

References

  • Frost AR, Sparks D, Grizzle WE. Methods of antigen recovery vary in their usefulness in unmasking speci- fic antigens in immunohistochemistry. Appl Immuno- histochem Mol Morphol 2000;8:236-43.
  • Grizzle WE, Manne U, Jhala NC, Weiss HL. Molecular characterization of colorectal neoplasia in translational research. Arch Pathol Lab Med 2001;125:91-8.
  • Schuknecht HF. Methods of removal, preparation and study. In: Schuknecht HF, editor. Pathology of the ear. 2nd ed. Philadelphia: Lea and Febiger; 1993. p. 1-27.
  • Keithley EM, Truong T, Chandronait B, Billings PB. Immunohistochemistry and microwave decalcification of human temporal bones. Hear Res 2000;148:192-6.
  • Cunningham CD 3rd, Schulte BA, Bianchi LM, Weber PC, Schmiedt BN. Microwave decalcification of human temporal bones. Laryngoscope 2001;111:278-82.
  • Schuknecht HF. Light and electron microscopy on the same temporal bone. Ann Otol Rhinol Laryngol Suppl. 1989;143:40.
  • Ishiyama A, Lopez I, Wackym PA. Choline acetyl- transferase immunoreactivity in the human vestibular end-organs. Cell Biol Int 1994;18:979-84.
  • Wackym PA. Molecular temporal bone pathology: I. Historical foundation. Laryngoscope 1997;107:1156-64.
  • Tian Q, Linthicum FH Jr, Keithley EM. Application of labeling techniques to archival temporal bone sections. Ann Otol Rhinol Laryngol 1999;108:47-53.
  • Shi SR, Cote RJ, Taylor CR. Antigen retrieval immuno- histochemistry used for routinely processed celloidin- embedded human temporal bone sections: standard- ization and development. Auris Nasus Larynx 1998; 25:425-43.
  • Shi SR, Cote RJ, Taylor CR. Antigen retrieval tech- niques: current perspectives. J Histochem Cytochem 2001;49:931-7.
  • Shi SR, Key ME, Kalra KL. Antigen retrieval in forma- lin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 1991;39:741-8.
  • Shi SR, Cote C, Kalra KL, Taylor CR, Tandon AK. A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry. J Histochem Cytochem 1992;40:787-92.
  • Shi SR, Tian Q. Development of an antigen retrieval technique for immunohistochemistry on archival cel- loidin-embedded sections. J Histochem Cytochem 1993;41:1121.
  • Ganbo T, Sando I, Balaban CD, Suzuki C, Sudo M. Im- munohistochemistry of lymphocytes and macrophages in human celloidin-embedded temporal bone sections with acute otitis media. Ann Otol Rhinol Laryngol 1997;106:662-8.
  • Moore JK, Guan YL, Shi SR. Axogenesis in the human fetal auditory system, demonstrated by neurofilament immunohistochemistry. Anat Embryol (Berl) 1997; 195:15-30.
  • Wackym PA, Simpson TA, Gantz BJ, Smith RJ. Polymerase chain reaction amplification of DNA from archival celloidin-embedded human temporal bone sections. Laryngoscope 1993;103:583-8.
  • Wackym PA, Popper P, Kerner MM, Grody WW. Varicella-zoster DNA in temporal bones of patients with Ramsay Hunt syndrome. Lancet 1993;342:1555.
  • Kerner MM, Wackym PA, Popper P, Tabor DE, Grody WW. Cloning and sequencing of genomic DNA extracted from archival human temporal bone sec- tions. Laryngoscope 1994;104:127-34.
  • Wackym PA. Molecular temporal bone pathology: II. Ramsay Hunt syndrome (herpes zoster oticus). Laryngoscope 1997;107:1165-75.
  • Burgess RC, Michaels L, Bale JF Jr, Smith RJ. Polymerase chain reaction amplification of herpes sim- plex viral DNA from the geniculate ganglion of a patient with Bell’s palsy. Ann Otol Rhinol Laryngol 1994;103:775-9.
  • Arbusow V, Theil D, Strupp M, Mascolo A, Brandt T. HSV-1 not only in human vestibular ganglia but also in the vestibular labyrinth. Audiol Neurootol 2001;6: 259-62.
  • Carreno M, Ona M, Melon S, Llorente JL, Diaz JJ, Suarez C. Amplification of herpes simplex virus type 1 DNA in human geniculate ganglia from formalin- fixed, nonembedded temporal bones. Otolaryngol Head Neck Surg 2000;123:508-11.
  • Linthicum F. Herpes simplex virus DNA in endolym- phatic sacs in patients with Meniere’s disease. Nat Temp Bone Registry 2002;9:4.
  • Bachor E, Sudhoff H, Litschel R, Karmody CS. The pathology of the temporal bones of a child with acquired cytomegalovirus infection: studies by light microscopy, immunohistochemistry and polymerase- chain reaction. Int J Pediatr Otorhinolaryngol 2000; 55:215-24.
  • McKenna MJ, Kristiansen AG, Haines J. Polymerase chain reaction amplification of a measles virus sequence from human temporal bone sections with active otosclerosis. Am J Otol 1996;17:827-30.
  • Niedermeyer HP, Arnold W, Neubert WJ, Sedlmeier R. Persistent measles virus infection as a possible cause of otosclerosis: state of the art. Ear Nose Throat J 2000;79:552-4, 556, 558 passim.
  • Marioni G, Altavilla G, Busatto G, Blandamura S, De Filippis C, Staffieri A. Detection of human papillo- mavirus in temporal bone inverted papilloma by poly- merase chain reaction. Acta Otolaryngol 2003;123:367-71.
  • Karosi T, Konya J, Szabo LZ, Sziklai I. Measles virus prevalence in otosclerotic stapes footplate samples. Otol Neurotol 2004;25:451-6.
  • Wackym PA, Kerner MM, Grody WW. Molecular tem- poral bone pathology: III. Genotyping of the deltaF508 deletion in the DNA of patients with cystic fibrosis. Laryngoscope 1998;108(8 Pt 2 Suppl 88):1-3.
  • Seidman MD, Bai U, Khan MJ, Murphy MJ, Quirk WS, Castora FL, et al. Association of mitochondrial DNA deletions and cochlear pathology: a molecular biolog- ic tool. Laryngoscope 1996;106:777-83.
  • Bai U, Seidman MD, Hinojosa R, Quirk WS. Mitochondrial DNA deletions associated with aging and possibly presbycusis: a human archival temporal bone study. Am J Otol 1997;18:449-53.
  • Dai P, Yang W, Jiang S, Gu R, Yuan H, Han D, et al. Correlation of cochlear blood supply with mitochon- drial DNA common deletion in presbyacusis. Acta Otolaryngol 2004;124:130-6.
  • McKenna MJ, Kristiansen AG, Tropitzsch AS, Tranebjaerg L, Merchant SN. Deoxyribonucleic acid contamination in archival human temporal bones: a potentially significant problem. Otol Neurotol 2002; 23:789-92.
  • McKenna M, Kristiansen AG. Nat Temp Bone Registry 2002;10:6.
  • Ohtani F, Furuta Y, Iino Y, Inuyama Y, Fukuda S. Amplification of RNA from archival human temporal bone sections. Laryngoscope 1999;109:617-20.
  • Lin J, Kawano H, Paparella MM, Ho SB. Improved RNA analysis for immediate autopsy of temporal bone soft tissues. Acta Otolaryngol 1999;119:787-95.

İnsan temporal kemik laboratuvarı prosedürleri: Genel bir bakış

Year 2006, Volume: 16 Issue: 5, 235 - 240, 14.10.2006

Abstract

İnsan kulağındaki yapılar genellikle yaşam boyunca “erişilemez” özellikte olup, altta yatan hastalıklara ait patolojik değişikliklerin incelenebilmesi postmortem çalışmalarla mümkün olabilmektedir. İnsan temporal kemik İTK laboratuvarları bu açıdan eşsiz bir ma- teryal kaynağı sunarlar. Bu laboratuvarlar, temporal kemik kesitlerindeki histolojik bulgularla hastanın öl- meden önceki kulak patolojisinin karşılaştırılmasına, hastalıklı olguları diğer olgularla karşılaştırmaya ve elde edilen bilgilerin diğer laboratuvarlarla paylaşıl- masına olanak verir. Bu makalede insan temporal kemik laboratuvarlarının işlevinin tanıtılması amaç- landı ve yapılan histopatolojik işlemlerin ayrıntılı analiziyle birlikte bu laboratuvarlar hakkında genel bir bakış açısı sunuldu

References

  • Frost AR, Sparks D, Grizzle WE. Methods of antigen recovery vary in their usefulness in unmasking speci- fic antigens in immunohistochemistry. Appl Immuno- histochem Mol Morphol 2000;8:236-43.
  • Grizzle WE, Manne U, Jhala NC, Weiss HL. Molecular characterization of colorectal neoplasia in translational research. Arch Pathol Lab Med 2001;125:91-8.
  • Schuknecht HF. Methods of removal, preparation and study. In: Schuknecht HF, editor. Pathology of the ear. 2nd ed. Philadelphia: Lea and Febiger; 1993. p. 1-27.
  • Keithley EM, Truong T, Chandronait B, Billings PB. Immunohistochemistry and microwave decalcification of human temporal bones. Hear Res 2000;148:192-6.
  • Cunningham CD 3rd, Schulte BA, Bianchi LM, Weber PC, Schmiedt BN. Microwave decalcification of human temporal bones. Laryngoscope 2001;111:278-82.
  • Schuknecht HF. Light and electron microscopy on the same temporal bone. Ann Otol Rhinol Laryngol Suppl. 1989;143:40.
  • Ishiyama A, Lopez I, Wackym PA. Choline acetyl- transferase immunoreactivity in the human vestibular end-organs. Cell Biol Int 1994;18:979-84.
  • Wackym PA. Molecular temporal bone pathology: I. Historical foundation. Laryngoscope 1997;107:1156-64.
  • Tian Q, Linthicum FH Jr, Keithley EM. Application of labeling techniques to archival temporal bone sections. Ann Otol Rhinol Laryngol 1999;108:47-53.
  • Shi SR, Cote RJ, Taylor CR. Antigen retrieval immuno- histochemistry used for routinely processed celloidin- embedded human temporal bone sections: standard- ization and development. Auris Nasus Larynx 1998; 25:425-43.
  • Shi SR, Cote RJ, Taylor CR. Antigen retrieval tech- niques: current perspectives. J Histochem Cytochem 2001;49:931-7.
  • Shi SR, Key ME, Kalra KL. Antigen retrieval in forma- lin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 1991;39:741-8.
  • Shi SR, Cote C, Kalra KL, Taylor CR, Tandon AK. A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry. J Histochem Cytochem 1992;40:787-92.
  • Shi SR, Tian Q. Development of an antigen retrieval technique for immunohistochemistry on archival cel- loidin-embedded sections. J Histochem Cytochem 1993;41:1121.
  • Ganbo T, Sando I, Balaban CD, Suzuki C, Sudo M. Im- munohistochemistry of lymphocytes and macrophages in human celloidin-embedded temporal bone sections with acute otitis media. Ann Otol Rhinol Laryngol 1997;106:662-8.
  • Moore JK, Guan YL, Shi SR. Axogenesis in the human fetal auditory system, demonstrated by neurofilament immunohistochemistry. Anat Embryol (Berl) 1997; 195:15-30.
  • Wackym PA, Simpson TA, Gantz BJ, Smith RJ. Polymerase chain reaction amplification of DNA from archival celloidin-embedded human temporal bone sections. Laryngoscope 1993;103:583-8.
  • Wackym PA, Popper P, Kerner MM, Grody WW. Varicella-zoster DNA in temporal bones of patients with Ramsay Hunt syndrome. Lancet 1993;342:1555.
  • Kerner MM, Wackym PA, Popper P, Tabor DE, Grody WW. Cloning and sequencing of genomic DNA extracted from archival human temporal bone sec- tions. Laryngoscope 1994;104:127-34.
  • Wackym PA. Molecular temporal bone pathology: II. Ramsay Hunt syndrome (herpes zoster oticus). Laryngoscope 1997;107:1165-75.
  • Burgess RC, Michaels L, Bale JF Jr, Smith RJ. Polymerase chain reaction amplification of herpes sim- plex viral DNA from the geniculate ganglion of a patient with Bell’s palsy. Ann Otol Rhinol Laryngol 1994;103:775-9.
  • Arbusow V, Theil D, Strupp M, Mascolo A, Brandt T. HSV-1 not only in human vestibular ganglia but also in the vestibular labyrinth. Audiol Neurootol 2001;6: 259-62.
  • Carreno M, Ona M, Melon S, Llorente JL, Diaz JJ, Suarez C. Amplification of herpes simplex virus type 1 DNA in human geniculate ganglia from formalin- fixed, nonembedded temporal bones. Otolaryngol Head Neck Surg 2000;123:508-11.
  • Linthicum F. Herpes simplex virus DNA in endolym- phatic sacs in patients with Meniere’s disease. Nat Temp Bone Registry 2002;9:4.
  • Bachor E, Sudhoff H, Litschel R, Karmody CS. The pathology of the temporal bones of a child with acquired cytomegalovirus infection: studies by light microscopy, immunohistochemistry and polymerase- chain reaction. Int J Pediatr Otorhinolaryngol 2000; 55:215-24.
  • McKenna MJ, Kristiansen AG, Haines J. Polymerase chain reaction amplification of a measles virus sequence from human temporal bone sections with active otosclerosis. Am J Otol 1996;17:827-30.
  • Niedermeyer HP, Arnold W, Neubert WJ, Sedlmeier R. Persistent measles virus infection as a possible cause of otosclerosis: state of the art. Ear Nose Throat J 2000;79:552-4, 556, 558 passim.
  • Marioni G, Altavilla G, Busatto G, Blandamura S, De Filippis C, Staffieri A. Detection of human papillo- mavirus in temporal bone inverted papilloma by poly- merase chain reaction. Acta Otolaryngol 2003;123:367-71.
  • Karosi T, Konya J, Szabo LZ, Sziklai I. Measles virus prevalence in otosclerotic stapes footplate samples. Otol Neurotol 2004;25:451-6.
  • Wackym PA, Kerner MM, Grody WW. Molecular tem- poral bone pathology: III. Genotyping of the deltaF508 deletion in the DNA of patients with cystic fibrosis. Laryngoscope 1998;108(8 Pt 2 Suppl 88):1-3.
  • Seidman MD, Bai U, Khan MJ, Murphy MJ, Quirk WS, Castora FL, et al. Association of mitochondrial DNA deletions and cochlear pathology: a molecular biolog- ic tool. Laryngoscope 1996;106:777-83.
  • Bai U, Seidman MD, Hinojosa R, Quirk WS. Mitochondrial DNA deletions associated with aging and possibly presbycusis: a human archival temporal bone study. Am J Otol 1997;18:449-53.
  • Dai P, Yang W, Jiang S, Gu R, Yuan H, Han D, et al. Correlation of cochlear blood supply with mitochon- drial DNA common deletion in presbyacusis. Acta Otolaryngol 2004;124:130-6.
  • McKenna MJ, Kristiansen AG, Tropitzsch AS, Tranebjaerg L, Merchant SN. Deoxyribonucleic acid contamination in archival human temporal bones: a potentially significant problem. Otol Neurotol 2002; 23:789-92.
  • McKenna M, Kristiansen AG. Nat Temp Bone Registry 2002;10:6.
  • Ohtani F, Furuta Y, Iino Y, Inuyama Y, Fukuda S. Amplification of RNA from archival human temporal bone sections. Laryngoscope 1999;109:617-20.
  • Lin J, Kawano H, Paparella MM, Ho SB. Improved RNA analysis for immediate autopsy of temporal bone soft tissues. Acta Otolaryngol 1999;119:787-95.
There are 37 citations in total.

Details

Primary Language Turkish
Journal Section Collection
Authors

Mehmet Faruk Oktay This is me

Sebahattin Cüreoğlu This is me

Patricia A. Schachern This is me

Micheal Paparella This is me

Publication Date October 14, 2006
Published in Issue Year 2006 Volume: 16 Issue: 5

Cite

APA Oktay, M. F., Cüreoğlu, S., Schachern, P. A., Paparella, M. (2006). İnsan temporal kemik laboratuvarı prosedürleri: Genel bir bakış. The Turkish Journal of Ear Nose and Throat, 16(5), 235-240.
AMA Oktay MF, Cüreoğlu S, Schachern PA, Paparella M. İnsan temporal kemik laboratuvarı prosedürleri: Genel bir bakış. Tr-ENT. October 2006;16(5):235-240.
Chicago Oktay, Mehmet Faruk, Sebahattin Cüreoğlu, Patricia A. Schachern, and Micheal Paparella. “İnsan Temporal Kemik Laboratuvarı prosedürleri: Genel Bir bakış”. The Turkish Journal of Ear Nose and Throat 16, no. 5 (October 2006): 235-40.
EndNote Oktay MF, Cüreoğlu S, Schachern PA, Paparella M (October 1, 2006) İnsan temporal kemik laboratuvarı prosedürleri: Genel bir bakış. The Turkish Journal of Ear Nose and Throat 16 5 235–240.
IEEE M. F. Oktay, S. Cüreoğlu, P. A. Schachern, and M. Paparella, “İnsan temporal kemik laboratuvarı prosedürleri: Genel bir bakış”, Tr-ENT, vol. 16, no. 5, pp. 235–240, 2006.
ISNAD Oktay, Mehmet Faruk et al. “İnsan Temporal Kemik Laboratuvarı prosedürleri: Genel Bir bakış”. The Turkish Journal of Ear Nose and Throat 16/5 (October 2006), 235-240.
JAMA Oktay MF, Cüreoğlu S, Schachern PA, Paparella M. İnsan temporal kemik laboratuvarı prosedürleri: Genel bir bakış. Tr-ENT. 2006;16:235–240.
MLA Oktay, Mehmet Faruk et al. “İnsan Temporal Kemik Laboratuvarı prosedürleri: Genel Bir bakış”. The Turkish Journal of Ear Nose and Throat, vol. 16, no. 5, 2006, pp. 235-40.
Vancouver Oktay MF, Cüreoğlu S, Schachern PA, Paparella M. İnsan temporal kemik laboratuvarı prosedürleri: Genel bir bakış. Tr-ENT. 2006;16(5):235-40.