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Lipid peroxidation and antioxidant levels in patients with laryngeal carcinoma

Year 2007, Volume: 17 Issue: 2, 81 - 84, 18.03.2007

Yavuz Fuat Yılmaz Filiz Akbıyık Ümit Tunçel Adnan Ünal

Abstract

Objectives: Reactive oxygen species ROS play an important role in the pathogenesis of cancer. The aim of this study was to investigate the extent of lipid peroxidation and ROS in laryngeal cancer tissues.Patients and Methods: We determined glutathione GSH and malondialdehyde MDA activities as markers of lipid peroxidation in laryngeal tumor spec- imens and tumor-free adjacent tissues of 30 patients with squamous celi carcinoma.Results: Compared to the tumor-free specimens, the level of GSH was significantly low p<0.001 whereas MDA, a lipid peroxidation product, showed a significant increase p<0.01 in cancer tissues. No significant differences were found in MDA and GSH levels between patients with early n=14 and advanced n=16 tumor stages p>0.05 .Conclusions: Decreased antioxidant capacity of laryngeal cancer tissues results in elevation of free oxygen radicals and increased lipid peroxida- tion. Free radical metabolism may be involved in the pathogenesis of laryngeal cancers.

Keywords

Antioxidants/metabolism carcinoma squamous celi free radicals

References

  • Bandyopadhyay U, Das D, Banerjee RK. Reactive oxy- gen species: oxidative damage and pathogenesis. Curr Sci 1999;77:658-66.
  • Burdon RH, Gill V, Rice-Evans C. Cell proliferation and oxidative stress. Free Radic Res Commun 1989; 7:149-59.
  • Kinsler TW, Taffe BG. Free radicals in tumor promo- tion. Adv Free Rad Biol Med 1986;2:347-88.
  • Comstock GW, Alberg AJ, Huang HY, Wu K, Burke AE, Hoffman SC, et al. The risk of developing lung cancer associated with antioxidants in the blood: ascorbic acid, carotenoids, alpha-tocopherol, selenium, and total peroxyl radical absorbing capacity. Cancer Epidemiol Biomarkers Prev 1997;6:907-16.
  • Perchellet JP, Perchellet EM. Antioxidants and multi- stage carcinogenesis in mouse skin. Free Radic Biol Med 1989;7:377-408.
  • Borochov H, Abbott RE, Schachter D, Shinitzky M. Modulation of erythrocyte membrane proteins by membrane cholesterol and lipid fluidity. Biochemistry 1979;18:251-5.
  • Farooqui MY, Day WW, Zamorano DM. Glutathione and lipid peroxidation in the aging rat. Comp Biochem Physiol B. 1987;88:177-80.
  • Halliwell B, Gutteridge JM, editors. Free radicals in biology and medicine. Oxford: Clarendon Press; 1989.
  • Durak I, Isik AC, Canbolat O, Akyol O, Kavutcu M. Adenosine deaminase, 5’ nucleotidase, xanthine oxi- dase, superoxide dismutase, and catalase activities in cancerous and noncancerous human laryngeal tissues. Free Radic Biol Med 1993;15:681-4.
  • Duthie GG, Arthur JR, James WP. Effects of smoking and vitamin E on blood antioxidant status. Am J Clin Nutr 1991;53(4 Suppl):1061-3.
  • Fleming ID, Cooper JS, Henson DE, Hutter RVP, Kennedy BJ, Murphy GP, editors. American Joint Committee cancer Staging manual. 5th ed. Philadelphia: Lippincott-Raven; 1997.
  • Harris CC. Interindividual variation among humans in carcinogen metabolism, DNA adduct formation and DNA repair. Carcinogenesis 1989;10:1563-6.
  • Nachiappan V, Mufti SI, Eskelson CD. Ethanol-mediated promotion of oral carcinogenesis in hamsters: association with lipid peroxidation. Nutr Cancer 1993;20:293-302.
  • Namyslowski G, Nowinska E, Danch A, Drozdz M, Wojcik A, Posielezna B, et al. A study of erythrocyte oxidative enzyme activity in patients with laryngeal cancer before and after treatment with a selenium preparation CEROSEL. Otolaryngol Pol 1995;49 Suppl 20:93-6. [Abstract]
  • Mihara M, Uchiyama M. Determination of malonalde- hyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978;86:271-8.
  • Tietze F. Enzymic method for quantitative determina- tion of nanogram amounts of total and oxidized glu- tathione: applications to mammalian blood and other tissues. Anal Biochem 1969;27:502-22.
  • Birnboim HC. DNA strand breaks in human leuko- cytes induced by superoxide anion, hydrogen perox- ide and tumor promoters are repaired slowly com- pared to breaks induced by ionizing radiation. Carcinogenesis 1986;7:1511-7.
  • Samir M, el Kholy NM. Thiobarbituric acid reactive substances in patients with laryngeal cancer. Clin Otolaryngol Allied Sci 1999;24:232-4.
  • Seven A, Civelek S, Inci E, Inci F, Korkut N, Burcak G. Evaluation of oxidative stress parameters in blood of patients with laryngeal carcinoma. Clin Biochem 1999; 32:369-73.
  • Yigitbasi OG, Guney E, Haghighi N, Dogan P, Saraymen R, Balkanli S. Oxidant and antioxidant sta- tus in larynx squamous cell carcinomas. J Exp Clin Cancer Res 2000;19:447-51.

Larenks karsinomlu hastalarda lipid peroksidasyonu ve antioksidan seviyeleri

Year 2007, Volume: 17 Issue: 2, 81 - 84, 18.03.2007

Yavuz Fuat Yılmaz Filiz Akbıyık Ümit Tunçel Adnan Ünal

Abstract

Amaç: Serbest oksijen radikalleri SOR kanser patogenezinde önemli rol oynamaktadır. Bu çalışmadalarengeal kanser dokusunda lipid peroksidasyonu veSOR seviyeleri araştırıldı.Hastalar ve Yöntemler: Larenks skuamöz hücrelikarsinomlu 30 hastada larengeal tümörlü ve tümörsüz dokuda glutatyon GSH ve malondialdehit MDA düzeyleri ve lipid peroksidasyonu değerlerikarşılaştırıldı.Bulgular: Glutatyon seviyesi kanserli dokuda belirgin olarak düşük bulundu p

Keywords

Antioksidan/metabolizma skuamöz hücreli kanser serbest radikal.

References

  • Bandyopadhyay U, Das D, Banerjee RK. Reactive oxy- gen species: oxidative damage and pathogenesis. Curr Sci 1999;77:658-66.
  • Burdon RH, Gill V, Rice-Evans C. Cell proliferation and oxidative stress. Free Radic Res Commun 1989; 7:149-59.
  • Kinsler TW, Taffe BG. Free radicals in tumor promo- tion. Adv Free Rad Biol Med 1986;2:347-88.
  • Comstock GW, Alberg AJ, Huang HY, Wu K, Burke AE, Hoffman SC, et al. The risk of developing lung cancer associated with antioxidants in the blood: ascorbic acid, carotenoids, alpha-tocopherol, selenium, and total peroxyl radical absorbing capacity. Cancer Epidemiol Biomarkers Prev 1997;6:907-16.
  • Perchellet JP, Perchellet EM. Antioxidants and multi- stage carcinogenesis in mouse skin. Free Radic Biol Med 1989;7:377-408.
  • Borochov H, Abbott RE, Schachter D, Shinitzky M. Modulation of erythrocyte membrane proteins by membrane cholesterol and lipid fluidity. Biochemistry 1979;18:251-5.
  • Farooqui MY, Day WW, Zamorano DM. Glutathione and lipid peroxidation in the aging rat. Comp Biochem Physiol B. 1987;88:177-80.
  • Halliwell B, Gutteridge JM, editors. Free radicals in biology and medicine. Oxford: Clarendon Press; 1989.
  • Durak I, Isik AC, Canbolat O, Akyol O, Kavutcu M. Adenosine deaminase, 5’ nucleotidase, xanthine oxi- dase, superoxide dismutase, and catalase activities in cancerous and noncancerous human laryngeal tissues. Free Radic Biol Med 1993;15:681-4.
  • Duthie GG, Arthur JR, James WP. Effects of smoking and vitamin E on blood antioxidant status. Am J Clin Nutr 1991;53(4 Suppl):1061-3.
  • Fleming ID, Cooper JS, Henson DE, Hutter RVP, Kennedy BJ, Murphy GP, editors. American Joint Committee cancer Staging manual. 5th ed. Philadelphia: Lippincott-Raven; 1997.
  • Harris CC. Interindividual variation among humans in carcinogen metabolism, DNA adduct formation and DNA repair. Carcinogenesis 1989;10:1563-6.
  • Nachiappan V, Mufti SI, Eskelson CD. Ethanol-mediated promotion of oral carcinogenesis in hamsters: association with lipid peroxidation. Nutr Cancer 1993;20:293-302.
  • Namyslowski G, Nowinska E, Danch A, Drozdz M, Wojcik A, Posielezna B, et al. A study of erythrocyte oxidative enzyme activity in patients with laryngeal cancer before and after treatment with a selenium preparation CEROSEL. Otolaryngol Pol 1995;49 Suppl 20:93-6. [Abstract]
  • Mihara M, Uchiyama M. Determination of malonalde- hyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978;86:271-8.
  • Tietze F. Enzymic method for quantitative determina- tion of nanogram amounts of total and oxidized glu- tathione: applications to mammalian blood and other tissues. Anal Biochem 1969;27:502-22.
  • Birnboim HC. DNA strand breaks in human leuko- cytes induced by superoxide anion, hydrogen perox- ide and tumor promoters are repaired slowly com- pared to breaks induced by ionizing radiation. Carcinogenesis 1986;7:1511-7.
  • Samir M, el Kholy NM. Thiobarbituric acid reactive substances in patients with laryngeal cancer. Clin Otolaryngol Allied Sci 1999;24:232-4.
  • Seven A, Civelek S, Inci E, Inci F, Korkut N, Burcak G. Evaluation of oxidative stress parameters in blood of patients with laryngeal carcinoma. Clin Biochem 1999; 32:369-73.
  • Yigitbasi OG, Guney E, Haghighi N, Dogan P, Saraymen R, Balkanli S. Oxidant and antioxidant sta- tus in larynx squamous cell carcinomas. J Exp Clin Cancer Res 2000;19:447-51.
There are 20 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Yavuz Fuat Yılmaz This is me

Filiz Akbıyık This is me

Ümit Tunçel This is me

Adnan Ünal This is me

Publication Date March 18, 2007
Published in Issue Year 2007 Volume: 17 Issue: 2

Cite

APA Yılmaz, Y. F., Akbıyık, F., Tunçel, Ü., Ünal, A. (2007). Larenks karsinomlu hastalarda lipid peroksidasyonu ve antioksidan seviyeleri. The Turkish Journal of Ear Nose and Throat, 17(2), 81-84.
AMA Yılmaz YF, Akbıyık F, Tunçel Ü, Ünal A. Larenks karsinomlu hastalarda lipid peroksidasyonu ve antioksidan seviyeleri. Tr-ENT. March 2007;17(2):81-84.
Chicago Yılmaz, Yavuz Fuat, Filiz Akbıyık, Ümit Tunçel, and Adnan Ünal. “Larenks Karsinomlu Hastalarda Lipid Peroksidasyonu Ve Antioksidan Seviyeleri”. The Turkish Journal of Ear Nose and Throat 17, no. 2 (March 2007): 81-84.
EndNote Yılmaz YF, Akbıyık F, Tunçel Ü, Ünal A (March 1, 2007) Larenks karsinomlu hastalarda lipid peroksidasyonu ve antioksidan seviyeleri. The Turkish Journal of Ear Nose and Throat 17 2 81–84.
IEEE Y. F. Yılmaz, F. Akbıyık, Ü. Tunçel, and A. Ünal, “Larenks karsinomlu hastalarda lipid peroksidasyonu ve antioksidan seviyeleri”, Tr-ENT, vol. 17, no. 2, pp. 81–84, 2007.
ISNAD Yılmaz, Yavuz Fuat et al. “Larenks Karsinomlu Hastalarda Lipid Peroksidasyonu Ve Antioksidan Seviyeleri”. The Turkish Journal of Ear Nose and Throat 17/2 (March 2007), 81-84.
JAMA Yılmaz YF, Akbıyık F, Tunçel Ü, Ünal A. Larenks karsinomlu hastalarda lipid peroksidasyonu ve antioksidan seviyeleri. Tr-ENT. 2007;17:81–84.
MLA Yılmaz, Yavuz Fuat et al. “Larenks Karsinomlu Hastalarda Lipid Peroksidasyonu Ve Antioksidan Seviyeleri”. The Turkish Journal of Ear Nose and Throat, vol. 17, no. 2, 2007, pp. 81-84.
Vancouver Yılmaz YF, Akbıyık F, Tunçel Ü, Ünal A. Larenks karsinomlu hastalarda lipid peroksidasyonu ve antioksidan seviyeleri. Tr-ENT. 2007;17(2):81-4.