Serum nitric oxide levels in patients with head and neck squamous celi carcinoma

Year 2007, Volume: 17 Issue: 3, 148 - 151, 23.05.2007

Ahmet Kızılay Mahmut Tayyar Kalcıoğlu Fikret Özuğurlu Hüseyin Özyurt İbrahim Aladağ Orhan Özturan Ömer Akyol

Abstract

Objectives: We determined serum nitric oxide NO levels in patients with head and neck squamous celi carcinoma SCO and sought correlations with TNM staging, tumor localization, and tumor grade.Patients and Methods: Serum samples were obtained from 36 patients mean age 63 years; range 37 to 80 years with head and neck SCO prior to treat- ment and from 20 healthy individuals mean age 56 years; range 30 to 72 years as Controls. Tumor stag­ ing was based on the criteria of the American Joint Committee of Cancer staging system in 2002. Thirteen patients had stage l-ll, and 23 patients had stage lll-IV tumors and ali had well- or moderately-differentiated SCO grade 1-2 . Serum NO levels were analyzed by a spectrophotometric method based on the determi- nation of total nitrite levels in serum and compared between the patient and control groups.Results: The mean serum NO levels were 20.08±1.40 pmol/l and 13.57±0.99 pmol/l in cancer patients and Controls, respectively p=0.001 . There were no correlations between NO levels and age, sex, tumor stage, localization, and histological grade.Conclusion: These data suggest that head and neck SCO is associated with increased serum NO levels, which may play a role in tumor growth.

Keywords

Carcinoma, squamous cell/metabolism, head and neck neoplasms/metabolism, nitric oxide/blood, tumor markers, biological

Baş-boyun yassı epitel hücreli kanserli hastaların serumlarında nitrik oksit düzeyleri

Abstract

Amaç: Baş-boyunda skuamöz hücreli kanser saptanan hastalarda serum nitrik oksit NO düzeyi belirlendi ve bunun TNM evresi, tümör yerleşimi ve derecelendirmesi ile ilişkisi araştırıldı.Hastalar ve Yöntemler: Baş-boyunda skuamözhücreli kanser tanısı konan 36 hastadan tedavi öncesinde ve kontrol olarak 20sağlıklı erişkinden serumörnekleri alındı. Tümör evrelemesi AJCC AmericanJoint Committee of Cancer 2002 ölçütlerine göreyapıldı. On üç hastada evre I-II, 23 hastada evre IIIIV tümör vardı. Tüm hastalarda tümör diferensiyasyonu iyi veya orta derecedeydi . SerumNO düzeylerinin analizi, serumda total nitritin saptanması esasına dayanan spektrofotometrik yöntemle yapıldı ve hasta ve kontrol gruplarının NO düzeyleri karşılaştırıldı.Bulgular: Kanserli grupta ve kontrol grubunda ortalama serum NO düzeyi sırasıyla 20.08±1.40 μmol/lve 13.57±0.99 μmol/l bulundu p=0.001 . Kanserligrupta NO düzeyi yaş, cinsiyet, tümör evresi, yerleşimi ve histolojik derecesi ile ilişkili bulunmadı.Sonuç: Bu veriler baş-boyundaki skuamöz hücreli kanserlerde serum NO düzeyinin arttığını göstermektedir.Bu aktivite artışı tümör büyümesinde rol oynayabilir

Keywords

Karsinom, skuamöz hücreli/metabolizma, baş-boyun neoplazileri/metabolizma, nitrik oksit/kan, tümör belirteci, biyolojik

References

• Moncada S, Palmer RM, Higgs EA. Nitric oxide: physi- ology, pathophysiology, and pharmacology. Pharmacol Rev 1991;43:109-42.
• Farrell AJ, Blake DR. Nitric oxide. Ann Rheum Dis 1996;55:7-20.
• Brennan PA. The actions and interactions of nitric oxide in solid tumours. Eur J Surg Oncol 2000;26:434-7.
• Gavilanes J, Moro MA, Lizasoain I, Lorenzo P, Perez A, Leza JC, et al. Nitric oxide synthase activity in human squamous cell carcinoma of the head and neck. Laryngoscope 1999;109:148-52.
• Farias-Eisner R, Sherman MP, Aeberhard E, Chaudhuri G.Nitric oxide is an important mediator for tumoricidal activity in vivo. Proc Natl Acad Sci U S A 1994;91:9407-11.
• Brennan PA, Downie IP, Langdon JD, Zaki GA. Emerging role of nitric oxide in cancer. Br J Oral Maxillofac Surg 1999;37:370-3.
• Pukkila MJ, Kellokoski JK, Virtaniemi JA, Kumpulainen EJ, Johansson RT, Halonen PM, et al. Inducible nitric oxide synthase expression in pharyn- geal squamous cell carcinoma: relation to p53 expression, clinicopathological data, and survival. Laryngoscope 2002;112:1084-8.
• Gallo O, Masini E, Morbidelli L, Franchi A, Fini- Storchi I, Vergari WA, et al. Role of nitric oxide in angiogenesis and tumor progression in head and neck cancer. J Natl Cancer Inst 1998;90:587-96.
• Koltuksuz U, Irmak MK, Karaman A, Uz E, Var A, Ozyurt H, Akyol O. Testicular nitric oxide levels after unilateral testicular torsion/detorsion in rats pretreat- ed with caffeic acid phenethyl ester. Urol Res 2000;28: 360-3.
• Akyol O, Herken H, Uz E, Fadillioglu E, Unal S, Sogut S, et al. The indices of endogenous oxidative and antiox- idative processes in plasma from schizophrenic patients. The possible role of oxidant/antioxidant imbalance. Prog Neuropsychopharmacol Biol Psychiatry 2002;26: 995-1005.
• Cortas NK, Wakid NW. Determination of inorganic nitrate in serum and urine by a kinetic cadmium- reduction method. Clin Chem 1990;36(8 Pt 1):1440-3.
• Rosbe KW, Prazma J, Petrusz P, Mims W, Ball SS, Weissler MC. Immunohistochemical characterization of nitric oxide synthase activity in squamous cell carci- noma of the head and neck. Otolaryngol Head Neck Surg 1995;113:541-9.
• Chen YK, Hsue SS, Lin LM. Increased expression of inducible nitric oxide synthase for human buccal squa- mous-cell carcinomas: immunohistochemical, reverse transcription-polymerase chain reaction (RT-PCR) and in situ RT-PCR studies. Head Neck 2002;24:925-32.
• Franchi A, Gallo O, Paglierani M, Sardi I, Magnelli L, Masini E, et al. Inducible nitric oxide synthase expres- sion in laryngeal neoplasia: correlation with angiogen- esis. Head Neck 2002;24:16-23.
• Wu NZ, Klitzman B, Dodge R, Dewhirst MW. Diminished leukocyte-endothelium interaction in tumor microvessels. Cancer Res 1992;52:4265-8.