The effect of intraperitoneal administration of zinc aspartate on myringosclerosis in perforated tympanic membranes of rats

Year 2009, Volume: 19 Issue: 5, 263 - 267, 23.10.2009

İlhami Yıldırım Harun Çıralık Erdoğan Okur Barlas Aydoğan M. Akif Kılıç

Abstract

Objectives: The objective of the present study was to determine the effect of zinc aspartate on myringosclerosis in perforated rat tympanic membrane. Patients and Methods: Fifteen Sprague-Dawley rats were randomly divided into three groups each containing five rats. Automicroscopic examinations were performed and then all rats were bilaterally myringotomized. Group 1 received no treatment. Group 2 was treated with intraperitoneal injection of physiological saline and group 3 with intraperitoneal injection of zinc aspartate. Tympanic bullas were harvested after 20 days. Histopathological evaluation was carried out under the light microscope. Results: When the groups were compared in the light of the myringosclerotic findings, while there was no significant difference between group 1 and 2 p=1.00 , it was found that there were significant differences between group 1 and 3, and between group 2 and 3 p<0.03 . Conclusion: It appears that zinc aspartat treatment has beneficial effects on prevention or retardation of the development of myringosclerosis, but further studies are needed to clarify this effect.

Keywords

Myringosclerosis, rat tympanic membrane, zinc aspartate

Sıçanlarda perfore timpanik membrandaki miringosklerozda intraperitoneal çinko aspartat uygulamasının etkisi

Abstract

Amaç: Bu çalışmada sıçan perfore timpanik membranındaki miringosklerozda çinko aspartatın etkisi araştırıldı.Hastalar ve Yöntemler: Sprague-Dawley cinsi 15 adet sıçan her bir grupta beş sıçan olacak şekilde rastgele üç gruba ayrıldı. Otomikroskopik muayeneleri yapıldıktan sonra sıçanlara iki taraflı miringotomi uygulandı. Grup 1’e hiçbir tedavi uygulanmadı, grup 2’ye intraperitoneal serum fizyolojik ve grup 3’e ise intraperitoneal çinko aspartat uygulandı. Timpanik bullalar 20 gün sonra çıkarıldı. Işık mikroskobu altında histopatolojik inceleme yapıldı.Bulgular: Miringosklerotik bulgulara göre gruplar karşılaştırıldığında, grup 1 ve 2 arasında anlamlı bir farklılık bulunmadı p=1.00 , grup 1 ile 3 arasında ve grup 2 ile 3 arasında anlamlı farklılıklar bulundu p

Keywords

Miringoskleroz, sıçan timpanik membran, çinko aspartat

References

• Tos M, Bonding P, Poulsen G. Tympanosclerosis of the drum in secretory otitis after insertion of grommets. A prospective, comparative study. J Laryngol Otol 1983;97:489-96.
• Mattsson C, Marklund SL, Hellström S. Application of oxygen free radical scavengers to diminish the occur- rence of myringosclerosis. Ann Otol Rhinol Laryngol 1997;106:513-8.
• Dursun G, Acar A, Turgay M, Calgüner M. Human leu- cocyte antigens in tympanosclerosis. Clin Otolaryngol Allied Sci 1997;22:62-4.
• Asiri S, Hasham A, al Anazy F, Zakzouk S, Banjar A. Tympanosclerosis: review of literature and incidence among patients with middle-ear infection. J Laryngol Otol 1999;113:1076-80.
• Akbaş Y, Pata YS, Görür K, Polat G, Polat A, Ozcan C, et al. The effect of L-carnitine on the prevention of experimentally induced myringosclerosis in rats. Hear Res 2003;184:107-12.
• Spratley JE, Hellström SO, Mattsson CK, Pais-Clemente M. Topical ascorbic acid reduces myringosclerosis in perforated tympanic membranes. A study in the rat. Ann Otol Rhinol Laryngol 2001;110:585-91.
• Görür K, Ozcan C, Polat A, Unal M, Tamer L, Cinel I. The anti-oxidant and anti-apoptotic activities of sele- nium in the prevention of myringosclerosis in rats. J Laryngol Otol 2002;116:426-9.
• Ozcan C, Görür K, Cinel L, Talas DU, Unal M, Cinel I. The inhibitory effect of topical N-acetylcysteine application on myringosclerosis in perforated rat tym- panic membrane. Int J Pediatr Otorhinolaryngol 2002; 63:179-84.
• Song JJ, Kwon SK, Cho CG, Park SW. The effect of caffeic acid phenethyl ester on the prevention of experimentally induced myringosclerosis. Int J Pediatr Otorhinolaryngol 2007;71:1287-91.
• Afanas’ev IB, Suslova TB, Cheremisina ZP, Abramova NE, Korkina LG. Study of antioxidant properties of metal aspartates. Analyst 1995;120:859-62.
• Ozkan KU, Boran C, Kilinç M, Garipardiç M, Kurutafl EB. The effect of zinc aspartate pretreatment on isch- emia-reperfusion injury and early changes of blood and tissue antioxidant enzyme activities after unilat- eral testicular torsion-detorsion. J Pediatr Surg 2004; 39:91-5.
• Ferlito A. Histopathogenesis of tympanosclerosis. J Laryngol Otol 1979;93:25-37.
• Felding JU, Rasmussen JB, Lildholdt T. Gas composi- tion of the normal and the ventilated middle ear cav- ity. Scand J Clin Lab Invest Suppl. 1987;186:31-41.
• Deneke SM, Fanburg BL. Normobaric oxygen toxicity of the lung. N Engl J Med 1980;303:76-86.
• Mattsson C, Magnuson K, Hellström S. Myringosclerosis caused by increased oxygen concentration in trauma- tized tympanic membranes. Experimental study. Ann Otol Rhinol Laryngol 1995;104:625-32.
• Mattsson C, Carlsson L, Marklund SL, Hellström S. Myringotomized mice develop myringosclerosis in the pars flaccida and not in the pars tensa. Laryngoscope 1997;107:200-5.
• Ozcan C, Polat G, Görür K, Talas DU, Bağdatoğlu O, Cinel I. The effect of local administration of N-acetylcysteine in perforated rat tympanic mem- brane: an experimental study in myringosclerosis. Pharmacol Res 2002;45:5-9.
• Boran C, Ozkan KU. The effect of zinc therapy on damaged testis in pre-pubertal rats. Pediatr Surg Int 2004;20:444-8.
• Yoshikawa T, Naito Y, Tanigawa T, Yoneta T, Yasuda M, Ueda S, et al. Effect of zinc-carnosine chelate com- pound (Z-103), a novel antioxidant, on acute gastric mucosal injury induced by ischemia-reperfusion in rats. Free Radic Res Commun 1991;14:289-96.
• Töns C, Klosterhalfen B, Klein HM, Rau HM, Anurov M, Oettinger A, et al. Induction of heat shock pro- tein 70 (HSP70) by zinc bis (DL-hydrogen aspartate) reduces ischemic small-bowel tissue damage in rats. Langenbecks Arch Chir 1997;382:43-8.
• Kadoya C, Domino EF, Yang GY, Stern JD, Betz AL. Preischemic but not postischemic zinc protoporphyrin treatment reduces infarct size and edema accumula- tion after temporary focal cerebral ischemia in rats. Stroke 1995;26:1035-8.
• Ophir A, Berenshtein E, Kitrossky N, Averbukh E. Protection of the transiently ischemic cat retina by zinc-desferrioxamine. Invest Ophthalmol Vis Sci 1994; 35:1212-22.