Does mitomycin-C increase collagen turnover as a modulator of wound healing in tracheostomyzed rats?

Ahmet Eyibilen; Mehmet Güven; İbrahim Aladağ; Hakan Kesici; Sema Koç; Levent Gürbüzler; Fatih Turan; Zafer İsmail Karaca; Hüseyin Özyurt

Mitomycin-C trakeotomi uygulanan sıçanlarda yara iyileşmesini düzenleyici olarak kollajen metabolizmasını artırır mı?

Year 2011, Volume: 21 Issue: 3, 154 - 158, 30.07.2011

Ahmet Eyibilen Mehmet Güven İbrahim Aladağ Hakan Kesici Sema Koç Levent Gürbüzler Fatih Turan Zafer İsmail Karaca Hüseyin Özyurt

Abstract

Amaç: Bu çalışmada trakeotomi uygulanan sıçanlarda, yara iyileşmesi sürecinde kollajen sentezi üzerine mitomycin-C’nin etkisi incelendi.Gereç ve Yöntem: On dört sağlıklı, her iki cinsiyette, ortalama ağırlıkları 270 g dağılım 250-300 g olan Wistar-Albino tipi sıçana trakeotomi uygulandı ve trakeal mukoza trakeotomi bölgesinin her iki tarafında mikromakasla hasarlandı. Sıçanlar iki gruba ayrıldı: Deney grubuna grup 1 derhal 0.2 mg/ml mitomycin-C ile topikal uygulama yapıldı; kontrol grubuna grup 2 ise izotonik salin solüsyonu uygulandı. Sıçanlar bir aylık süre sonunda sakrifiye edildi. Daha sonra, trakeotomi uygulanan bölge çıkarılarak vertikal olarak iki kısma ayrıldı. Dokunun bir kısmında kuru dokuda hidroksiprolin seviyesi ölçüldü. Trakeotomi yapılan diğer kısımda stereolojik yöntemle fibroblast sayımı yapıldı.Bulgular: Hidroksiprolin seviyesi mitomycin-C uygulanan grupta kontrol grubuna göre çok daha yüksekti p

Keywords

Kollajen sentezi, hidroksiprolin, mitomycin-C, trakeal stenoz

Does mitomycin-C increase collagen turnover as a modulator of wound healing in tracheostomyzed rats?

Year 2011, Volume: 21 Issue: 3, 154 - 158, 30.07.2011

Ahmet Eyibilen Mehmet Güven İbrahim Aladağ Hakan Kesici Sema Koç Levent Gürbüzler Fatih Turan Zafer İsmail Karaca Hüseyin Özyurt

Abstract

Objectives: This study aims to investigate the effect of mitomycin-C in the wound healing process on collagen synthesis in tracheostomyzed rats. Materials and Methods: Fourteen healthy, in both sexes, mean weight of 270 g range 250-300 g , WistarAlbino type rats underwent tracheotomy and tracheal mucosa was damaged with micro-scissors on both sides of tracheostomyzed area. The rats were divided into two groups: The experimental group group 1 received immediate topical application of mitomycin-C 0.2 mg/ml; the control group group 2 received saline solution. The rats were sacrificed after a period of one month. Subsequently, the tracheostomyzed region was excised and vertically divided into the two parts. The level of hydroxyproline in the dry tissue was measured in one part of the tissue. Fibroblast count was performed in the other part of the tracheostomyzed region using the stereological method. Results: The hydroxyproline level was much higher in the mitomycin-C group when compared to the control group p<0.05 . In contrary, the number of fibroblasts was lower in the mitomycin-C group than control group p<0.05 . Conclusion: When used in wound healing, mitomycin-C may increase collagen synthesis or quicken the wound healing process after one month.

Keywords

Collagen sythesis, hydroxyproline, mitomycin-C, tracheal stenosis

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Details

Primary Language	English
Journal Section	Research Article
Authors	Ahmet Eyibilen This is me Mehmet Güven This is me İbrahim Aladağ This is me Hakan Kesici This is me Sema Koç This is me Levent Gürbüzler This is me Fatih Turan This is me Zafer İsmail Karaca This is me Hüseyin Özyurt This is me
Publication Date	July 30, 2011
Published in Issue	Year 2011 Volume: 21 Issue: 3

Cite

APA	Eyibilen, A., Güven, M., Aladağ, İ., Kesici, H., et al. (2011). Does mitomycin-C increase collagen turnover as a modulator of wound healing in tracheostomyzed rats?. The Turkish Journal of Ear Nose and Throat, 21(3), 154-158.
AMA	Eyibilen A, Güven M, Aladağ İ, Kesici H, Koç S, Gürbüzler L, Turan F, Karaca Zİ, Özyurt H. Does mitomycin-C increase collagen turnover as a modulator of wound healing in tracheostomyzed rats?. Tr-ENT. July 2011;21(3):154-158.
Chicago	Eyibilen, Ahmet, Mehmet Güven, İbrahim Aladağ, Hakan Kesici, Sema Koç, Levent Gürbüzler, Fatih Turan, Zafer İsmail Karaca, and Hüseyin Özyurt. “Does Mitomycin-C Increase Collagen Turnover As a Modulator of Wound Healing in Tracheostomyzed Rats?”. The Turkish Journal of Ear Nose and Throat 21, no. 3 (July 2011): 154-58.
EndNote	Eyibilen A, Güven M, Aladağ İ, Kesici H, Koç S, Gürbüzler L, Turan F, Karaca Zİ, Özyurt H (July 1, 2011) Does mitomycin-C increase collagen turnover as a modulator of wound healing in tracheostomyzed rats?. The Turkish Journal of Ear Nose and Throat 21 3 154–158.
IEEE	A. Eyibilen, M. Güven, İ. Aladağ, H. Kesici, S. Koç, L. Gürbüzler, F. Turan, Z. İ. Karaca, and H. Özyurt, “Does mitomycin-C increase collagen turnover as a modulator of wound healing in tracheostomyzed rats?”, Tr-ENT, vol. 21, no. 3, pp. 154–158, 2011.
ISNAD	Eyibilen, Ahmet et al. “Does Mitomycin-C Increase Collagen Turnover As a Modulator of Wound Healing in Tracheostomyzed Rats?”. The Turkish Journal of Ear Nose and Throat 21/3 (July 2011), 154-158.
JAMA	Eyibilen A, Güven M, Aladağ İ, Kesici H, Koç S, Gürbüzler L, Turan F, Karaca Zİ, Özyurt H. Does mitomycin-C increase collagen turnover as a modulator of wound healing in tracheostomyzed rats?. Tr-ENT. 2011;21:154–158.
MLA	Eyibilen, Ahmet et al. “Does Mitomycin-C Increase Collagen Turnover As a Modulator of Wound Healing in Tracheostomyzed Rats?”. The Turkish Journal of Ear Nose and Throat, vol. 21, no. 3, 2011, pp. 154-8.
Vancouver	Eyibilen A, Güven M, Aladağ İ, Kesici H, Koç S, Gürbüzler L, Turan F, Karaca Zİ, Özyurt H. Does mitomycin-C increase collagen turnover as a modulator of wound healing in tracheostomyzed rats?. Tr-ENT. 2011;21(3):154-8.