Abstract
Objectives: The aim of this study was to investigate whether cyclooxygenase-2 COX-2 , arachidonate 12-lipoxygenase ALOX12 and inducible nitric oxide synthase iNOS which are well-known mediators in inflammatory process play a role in nasal polyposis NP and to show their roles in initiation and progression of inflammation. Patients and Methods: We investigated the expression levels of COX-2, ALOX12 and iNOS genes by Real-time polymerase chain reaction PCR method in NP and healthy nasal mucosa tissues obtained from 10 patients 4 females, 6 males; mean age 40.2 years; range 21 to 54 years . Results: The mRNA levels of COX-2 expression observed in NP was found to be relatively increased, compared to the control tissue p>0.05 . The ALOX12 levels were relatively decreased p>0.05 , while the expression level of iNOS mRNA was significantly higher in NP tissue p<0.05 . Conclusion: These data suggest that nitric oxide NO , a gene product of iNOS, may play a physiological role in the upper airways and also NO is associated with inflammatory processes in the airways.
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Abstract
Amaç: Çalışmamızda çeşitli enflamatuvar hastalıklarda etkileri bilinen siklooksijenaz-2 COX-2 , araşidonat 12-lipoksijenaz ALOX12 ve indüklenebilir nitrik oksit sentaz iNOS genlerinin nazal polip NP olgusunda etkisinin olup olmadığı araştırıldı ve enflamasyonun oluşumuna ya da sürdürülmesine nasıl bir katkıda bulundukları gösterildi.Hastalar ve Yöntemler: On hastadan 4 kadın, 6 erkek; ort. yaş 40.2 yıl; dağılım 21-54 yıl elde edilen NP ve sağlıklı nazal mukoza dokularında, gerçek zamanlı polimeraz zincir reaksiyonu Real-time PCR yöntemi ile COX-2, ALOX12 ve iNOS genlerinin mRNA ifadelenme düzeyleri araştırıldı.Bulgular: Nazal poliplerde gözlenen COX-2 ifadelenmesinin mRNA düzeyinin kontrol dokusuna göre kısmen artmış olduğu saptandı p>0.05 . ALOX12 ifadelenmesinin NP’lerde kısmen düştüğü p>0.05 , iNOS mRNA ifadelenme düzeyinin ise istatistiksel açıdan önemli ölçüde arttığı bulundu p
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Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | November 1, 2012 |
| Published in Issue | Year 2012 Volume: 22 Issue: 5 |