Ototoxic effect of topical rifamycin SV applied in the middle ear of rats

Year 2017, Volume: 27 Issue: 1, 31 - 38, 05.03.2017

Can Özbay Mustafa Şahin

Abstract

Objectives: In this study, we aimed to evaluate the ototoxic effect of topical rifamycin SV in the middle ear in a rat model. Materials and Methods: A total of 24 20-week-old adult female and eight 4-6-week-old weaned young, post-suckling period Wistar albino rats were used. Adult rats were separated into four groups: Group 1 adult rifamycin , group 2 weaner rifamycin , group 3 gentamicin; positive control , and group 4 saline; negative control . Before medication administration, an auditory brainstem response ABR test was performed on each animal under general anesthesia. Tympanic membranes of the animals were perforated, and the medications were administered to the middle ear for 10 days. Three weeks after treatment, ABR tests were repeated and pre- and posttreatment ABR threshold measurements were compared. Results: Although the mean pre- and post-treatment ABR threshold values did not significantly differ among groups 1, 2, and 4 p>0.05 , the mean post-treatment ABR threshold values were significantly higher in group 3 compared to baseline p<0.05 . Conclusion: Based on ABR measurements, the topical use of rifamycin SV, a broad-spectrum semi-synthetic antibiotic in the middle ear, does not cause hearing loss in adult or weaner rats.

Keywords

Auditory brainstem response, inner ear, ototoxicity, rifamycin SV

Sıçan orta kulağına uygulanan topikal rifamisin SV’nin ototoksik etkisi

Abstract

Amaç: Bu çalışmada bir sıçan modelinde topikal rifamisin SV’nin orta kulakta ototoksik etkisi araştırıldı.Gereç ve Yöntemler: Toplamda 24 adet, 20 haftalık erişkin dişi ve sekiz adet 4-6 haftalık sütten kesilmiş yavru Wistar albino sıçan kullanıldı. Erişkin sıçanlar dört gruba ayrıldı; Grup 1 erişkin rifamisin , grup 2 yavru rifamisin , grup 3 gentamisin; pozitif kontrol , grup 4 salin, negatif kontrol . İlaç uygulaması öncesinde her sıçana genel anestezi altında işitsel beyinsapı yanıtı ABR testi uygulandı. Hayvanların timpan membranları perfore edilerek, orta kulağa 10 gün boyunca ilaçlar uygulandı. Tedaviden üç hafta sonra ABR testleri tekrar edildi ve tedavi öncesi ve sonrası ABR eşik ölçümleri karşılaştırıldı.Bulgular: Tedavi öncesi ve tedavi sonrası ortalama ABR eşik değerleri grup 1, grup 2 ve grup 4’te istatistiksel olarak anlamlı farklılık göstermez iken p>0.05 , grup 3’te tedavi sonrası ortalama ABR eşik değerleri, başlangıca kıyasla, anlamlı düzeyde daha yüksekti p

Keywords

İşitsel beyinsapı yanıtı, iç kulak, ototoksisite, rifamisin SV

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