Combined Effect of Papain and Vitamin-C Levels on Growth Performance of Freshwater Giant Prawn, Macrobrachium rosenbergii
Abstract
Present study was conducted to evaluate the combined effect of proteolytic enzyme, papain and vitamin-C on freshwater giant prawn, Macrobrachium rosenbergii. Post-larvae of Macrobrachium rosenbergii of average weight 9.5-16.3 mg initial weight were fed with eight different diets supplemented with papain 0.0%, 0.108%, 0.3%, 0.5%, 0.0%, 0.108%, 0.3%, 0.5% and vitamin-C 0.0%, 0.0%, 0.0%, 0.0%, 0.2%, 0.2%, 0.2% and 0.2% for feeds F1 to F8 respectively. Before the start of the experiment, prawns were reared using papain and vitamin-C free diet in order to deplete their vitamin-C stores. The same diet formulation was used in all treatments containing soybean meal, fish meal, shrimp meal and wheat flour as the major ingredients. Feeding level was kept same for all the groups of prawn and fed to satiation. Protease specific activity after one hour of feeding at the end of the experiment was estimated, which have shown a higher value in comparison to the protease specific activity one hour before feeding at the beginning of the experiment. The results obtained after feeding trial demonstrated improved growth (P<0.05) at higher concentration (F7 and F8). However, the growth of post-larval stages of prawn fed only vitamin-C or papain showed lesser growth than those of the combined effect. Survival was recorded between 46-56% in all the treatments. No growth reduction or deficiency signs were observed. This finding is the first report of its kind where a combined effect of papain and vitamin-C showing a synergistic effect on the growth performance. Further standardization and commercialization of this finding, through economically viable methods, is needed to improve the growth performance of this premium commodity.
References
- Adham, K.G., Hashem, H.O., Abu-Shabana, M.B. and Kamel, A.H., 2000. Vitamin-C deficiency in catfish Clarias gariepinus. Aquaculture Nutrition, 6: 1291 doi: 10.1046/j.1365-2095.2000.00139.x
- Bennik, M. R., 1994. Fibre analysis. In: Introduction to chemical analysis of Foods - Suzanne, S. and Neilsen (Eds), 109 pp.
- Blom, J. H., Dabrowski, K. Rapp, J. D., Sakakura, Y. and Tsukamoto, 1999. Competition for space and food in Rainbow trout Onchorhynchus mykiss as related to ascorbic acid status, Aquaculture, 180: 79-87. doi: 1016/S0044-8486(99)00189-1
- Briggs, M. R. 1991. The performance of juvenile prawns, Macrobrachium rosenbergii, fed a range of carbohydrate source in semi purified diets. Journal of World Aquaculture. Society., 22(3): 16 A.
- Chang, S.K.C., 1994. Protein analysis. In: Introduction to chemical analysis of Foods- Suzanne, S. and Neilsen (Eds) 207 p.p.
- Cherry and Crandal, 1932. Determination of serum lipase activity, American. Journal of Physiology., 100: 266 p.p.
- Conklin, L.P. 1998. Vitamin-C: A new pathway from old antioxidant, Trends in Plant science, Research news, 3(9): 300-301.
- Corbin, J. S., Fugimoto, M. N. and iwaiIwai, T. Y. 1983. Feeding practices and nutritional consideration for Macrobrachium rosenbergii culture in Hawaii. Handbook of Mariculture, 1: Crustaceans Aquaculture. CRC Press Inc. Boca Rocton. Florida, 391 p.p.
- D’ Abramo, l.K. and Reed, L. 1988. Optimum dietary protein level for juvenile freshwater prawn Macrobrachium rosenbergii. 19 th annual meeting of the World Aquaculture Society, Hanolulu, Hawaii, 27 p.p.
- D’ Abramo, I. K. and Sheen, S.S. 1989. Essential fatty acid requirement of freshwater prawn Macrobrachium rosenbergii. 19 th annual meeting of the world Aquaculture society, Los Angeles, California, 35 p.p.
- D’ Abramo, L.R., Moncreiff, C.A., Holcomb, F.P., Montanez, J.L. and Buddangton, R.K. 1994. VitaminC requirement of Juvenile fresh water prawn, Macrobrachium rosenbergii. Aquaculture, 128: 2692
- Dabrowski, K.,1984. The feeding of fish larvae: present “state of the art” and perspectives, Reproduction, nutrition and development, Special publication, European Mariculture Society, 24: 807-833.
- Dabrowski, K., 1979, The role of proteolytic enzymes in fish digestion, In: E.Jaspers, and G.Persoone, (Eds) Cultivation of fish fry and its live food, Special publication European. Mariculture. Society. Special publication, 4:107-126.
- Dabrowski, K. and Glogowski, J. 1977a. Studies on the role of exogenous proteolytic enzymes in digestion process in fish. Hydrobiologia, 54(2): 129-134.
- Dabrowski, K. and Glogowski, J. 1977b. A study of application of proteolytic enzymes to fish food. Aquaculture, 12 : 349-360.
Combined Effect of Papain and Vitamin-C Levels on Growth Performance of Freshwater Giant Prawn, Macrobrachium rosenbergii
Abstract
Present study was conducted to evaluate the combined effect of proteolytic enzyme, papain and vitamin-C on freshwater giant prawn, Macrobrachium rosenbergii. Post-larvae of Macrobrachium rosenbergii of average weight 9.5-16.3 mg initial weight were fed with eight different diets supplemented with papain 0.0%, 0.108%, 0.3%, 0.5%, 0.0%, 0.108%, 0.3%, 0.5% and vitamin-C 0.0%, 0.0%, 0.0%, 0.0%, 0.2%, 0.2%, 0.2% and 0.2% for feeds F1 to F8 respectively. Before the start of the experiment, prawns were reared using papain and vitamin-C free diet in order to deplete their vitamin-C stores. The same diet formulation was used in all treatments containing soybean meal, fish meal, shrimp meal and wheat flour as the major ingredients. Feeding level was kept same for all the groups of prawn and fed to satiation. Protease specific activity after one hour of feeding at the end of the experiment was estimated, which have shown a higher value in comparison to the protease specific activity one hour before feeding at the beginning of the experiment. The results obtained after feeding trial demonstrated improved growth (P<0.05) at higher concentration (F7 and F8). However, the growth of post-larval stages of prawn fed only vitamin-C or papain showed lesser growth than those of the combined effect. Survival was recorded between 46-56% in all the treatments. No growth reduction or deficiency signs were observed. This finding is the first report of its kind where a combined effect of papain and vitamin-C showing a synergistic effect on the growth performance. Further standardization and commercialization of this finding, through economically viable methods, is needed to improve the growth performance of this premium commodity.
References
- Adham, K.G., Hashem, H.O., Abu-Shabana, M.B. and Kamel, A.H., 2000. Vitamin-C deficiency in catfish Clarias gariepinus. Aquaculture Nutrition, 6: 1291 doi: 10.1046/j.1365-2095.2000.00139.x
- Bennik, M. R., 1994. Fibre analysis. In: Introduction to chemical analysis of Foods - Suzanne, S. and Neilsen (Eds), 109 pp.
- Blom, J. H., Dabrowski, K. Rapp, J. D., Sakakura, Y. and Tsukamoto, 1999. Competition for space and food in Rainbow trout Onchorhynchus mykiss as related to ascorbic acid status, Aquaculture, 180: 79-87. doi: 1016/S0044-8486(99)00189-1
- Briggs, M. R. 1991. The performance of juvenile prawns, Macrobrachium rosenbergii, fed a range of carbohydrate source in semi purified diets. Journal of World Aquaculture. Society., 22(3): 16 A.
- Chang, S.K.C., 1994. Protein analysis. In: Introduction to chemical analysis of Foods- Suzanne, S. and Neilsen (Eds) 207 p.p.
- Cherry and Crandal, 1932. Determination of serum lipase activity, American. Journal of Physiology., 100: 266 p.p.
- Conklin, L.P. 1998. Vitamin-C: A new pathway from old antioxidant, Trends in Plant science, Research news, 3(9): 300-301.
- Corbin, J. S., Fugimoto, M. N. and iwaiIwai, T. Y. 1983. Feeding practices and nutritional consideration for Macrobrachium rosenbergii culture in Hawaii. Handbook of Mariculture, 1: Crustaceans Aquaculture. CRC Press Inc. Boca Rocton. Florida, 391 p.p.
- D’ Abramo, l.K. and Reed, L. 1988. Optimum dietary protein level for juvenile freshwater prawn Macrobrachium rosenbergii. 19 th annual meeting of the World Aquaculture Society, Hanolulu, Hawaii, 27 p.p.
- D’ Abramo, I. K. and Sheen, S.S. 1989. Essential fatty acid requirement of freshwater prawn Macrobrachium rosenbergii. 19 th annual meeting of the world Aquaculture society, Los Angeles, California, 35 p.p.
- D’ Abramo, L.R., Moncreiff, C.A., Holcomb, F.P., Montanez, J.L. and Buddangton, R.K. 1994. VitaminC requirement of Juvenile fresh water prawn, Macrobrachium rosenbergii. Aquaculture, 128: 2692
- Dabrowski, K.,1984. The feeding of fish larvae: present “state of the art” and perspectives, Reproduction, nutrition and development, Special publication, European Mariculture Society, 24: 807-833.
- Dabrowski, K., 1979, The role of proteolytic enzymes in fish digestion, In: E.Jaspers, and G.Persoone, (Eds) Cultivation of fish fry and its live food, Special publication European. Mariculture. Society. Special publication, 4:107-126.
- Dabrowski, K. and Glogowski, J. 1977a. Studies on the role of exogenous proteolytic enzymes in digestion process in fish. Hydrobiologia, 54(2): 129-134.
- Dabrowski, K. and Glogowski, J. 1977b. A study of application of proteolytic enzymes to fish food. Aquaculture, 12 : 349-360.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | June 1, 2013 |
| Published in Issue | Year 2013 |