Influence of Tetra (Cotinus coggygria) Extract against Vibrio Anguillarum Infection in Koi Carp, Cyprinus carpio with Reference to Haematological and Immunological Changes

Year 2013, , - , 01.06.2013

Soner Bilen Sevdan Yılmaz Aslı Müge Bilen

https://doi.org/10.4194/1303-2712-v13_3_16

Abstract

In this study, the effect of the methanolic extract of tetra (Cotinus coggygria) against Vibrio anguillarum in cultured koi carp (Cyprinus carpio carpio) was investigated. Three different concentrations of tetra extract (0, 0.5, 1, 1.5 g kg of feed) were individually mixed with the basal diet and fed to koi (average body weight of 4,14±0,08 g) for a 4-week period to assess the immunomodulatory characteristics of tetra. At the end of the study, koi was challenged with a strain of Vibrio anguillarum (10 CFU ml). Growth performance was not affected by dietary tetra extract intake (P>0.05). Nitroblue tetrazolium (NBT) activity was found to be the highest in the 1.5 g kgcontaining group. Lysozyme and myeloperoxidase activity of the experimental groups was significantly improved in the tetra groups compared to the control group (P<0.05). Red blood cell and mean corpuscular haemoglobin concentration (MCHC) were higher in 1 g kgtetra containing group (P<0.05). This study shows that the koi treated with 0, 0.5, 1 and 1.5 g kg tetra extract prior the challenge with live V. anguillarum had 37.50%, 31.94 %, 18.06 % and 12.50 % mortality. According to study results, tetra is an effective non-specific immunostimulant for koi.

Keywords

Koi carp, tetra, Cotinus coggygria, immunostimulant, Vibrio anguillarum, hematology.

Influence of Tetra (Cotinus coggygria) Extract against Vibrio Anguillarum Infection in Koi Carp, Cyprinus carpio with Reference to Haematological and Immunological Changes

Abstract

Bu çalışmada tetranın (Cotinus coggygria) metanolik özütünün koi balıklarında (Cyprinus carpio carpio) Vibrio anguillarum enfeksiyonuna karşı etkileri incelenmiştir. Koiler (ortalama ağırlıkları 4,14±0,08 g) üç farklı tetra dozu (0, 0,5, 1, 1,5 g kg) içeren yemlerle 4 hafta boyunca beslenmişlerdir. Çalışma sonunda balıklar Vibrio anguillarum ile enfekte edilerek kontrol testi yapılmıştır. Tetranın balıkların büyüme performansı üzerinde etkisi bulunamamıştır (P>0,05). 1,5 kg tetra verilen grupta NBT en yüksek olmuştur. Lizozim ve miyeloperoksidaz aktiviteleri tetra uygulanan gruplarda kontrol grubuna göre kayda değer yüksek bulunmuştur (P<0,05). Akyuvar ve hemoglobin konsantrasyonları 1 kg tetra içeren grupta yüksek bulunmuştur (P<0,05). 0, 0,5, 1 ve 1,5 g kg tetra ile beslenen gruplarda yapılan kontrol testlerinde ölüm oranları sırasıyla 37,50%, 31,94 %, 18,06 % ve 12,50 % olarak tespit edilmiştir. Çalışma sonuçlarına göre tetra koiler için etkili bir immunostimulanttır.

Keywords

Koi, tetra, Cotinus coggygria, immunostimulant, Vibrio anguillarum, hematoloji.

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