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New Mitochondrial DNA Haplotype of Brown Trout Salmo trutta l. from Crni Timok Drainage Area in Serbia

Year 2014, Volume: 14 Issue: 1, - , 07.07.2014

Ana Toši63; Dubravka Škraba Vera Nikoli63; Danilo Mrdak Predrag Simonovi63;

https://doi.org/10.4194/1303-2712-v14_1_05

Abstract

Brown trout Salmo trutta wild stocks sustain a remarkable angling and hatchery-reared fish stocking pressure in waters of Serbia, where four drainage-specific and indigenous mitochondrial DNA haplotypes were reported for the drainage area of the Danube River basin. One of these mitochondrial DNA haplotypes, Da23b, was exclusive for brown trout in headwaters of the Crni Timok River (Grand Timok River system, Eastern Serbia). After its discovery in 2003, brown trout stocking was completely halted and abandoned, and a strict Catch-and-Release fishing regime was issued. However, failure of this regime due to enforcement resulted in decline of brown trout fishery. On checking the aboriginality of brood fish sampled in three headwater forks for artificial brown trout propagation, a novel, drainage-specific mtDNA haplotype was recorded in 54% of brown trout. It was assigned Da23c, due to its similarity (substitutions at the two variable sites) to the hitherto known haplotypes of Da23 group. Its finding and absence of Da23b haplotype imply an oversight in earlier haplotype determination. Incorporation of the non-indigenous haplotypes Da2 and Da-s1, introduced in the area by stocking before 2003, into the gene pool of native brown trout and declining of the latter's stock imposes the need for the more stringently enforced conservational activities (genotyping of each brood fish involved in artificial propagation) and improved fisheries management (Catch-and-Release) in the area.

Keywords

D-loop Da23c Eastern Serbia conservation fisheries management

References

  • Apostolidis, A.P., Madeira, M.J., Hansen, M.M., and Machordom, A. 2008. Genetic structure and demographic history of brown trout (Salmo trutta L.) populations from the southern Balkans. Freshwater Biology, 53: 1555–1566. doi: 10.1111/j.1365242008.01988.x
  • Apostolidis, A.P., Stoumboudi, M.Th., Kalogianni, E., Cote, G., Bernatchez, L. 2011. Genetic divergence among native trout Salmo trutta populations from southern Balkans based on mitochondrial DNA and microsatellite variation. Journal of Fish Biology, 79: 1950–1960. doi:10.1111/j.1095-8649.2011.03136.x
  • Avise, J.C. 2000. Phylogeography: the History and Formation of Species. Harvard University Press, Cambridge.
  • Bernatchez, L. 2001. The evolutionary history of brown trout (Salmo trutta L.) inferred from phylogeographic, nested clade, and mismatch analyses of mitochondrial DNA variation. Evolution, 55: 351–379.
  • Bernatchez, L., and Danzmann, R.G. 1993. Congruence in control-region sequence and restriction–site variation in mitochondrial DNA of brook charr (Salvelinus fontinalis Mitchill). Molecular Biology and Evolution, 10: 1002–1014.
  • Bernatchez, L., Guyomard, R., and Bonhomme, F. 1992. DNA sequence variation of the mitochondrial control region among geographically and morphologically remote European brown trout Salmo trutta populations. Molecular Ecology, 1: 161–173.
  • Cortey, M., and García-Marín, J.L. 2002. Evidence for phylogeographically informative sequence variation in the mitochondrial control region of Atlantic brown trout. J. Fish Biol., 60: 1058–1063. doi:1006/jfbi.2002.1910, Duftner, N., Weiss, S., Medgyesy, N., Sturmbauer, C. 2003. Enhanced phylogeographic information about Austrian brown trout populations derived from complete mitochondrial control region sequences. J. Fish Biol., 62: 427–435. doi: 46/j.002212003.00038.x
  • Habibi, E., Kalbassi, M.R., Hosseini S.J., and Qasemi, S.A. 20 Feasibility of Identification of Fall and Spring Migrating Caspian trout (Salmo trutta caspius) by Using AFLP Molecular Marker. Turkish Journal of Fisheries and Aquatic Sciences, 13: 241-248. doi: 4194/1303-2712-v13_2_06
  • Kohout, J. 2012. Effects of stocking on the genetic structure of brown trout, Salmo trutta, in Central Europe inferred from mitochondrial and nuclear DNA markers. Turkish Journal of Fisheries and Aquatic Sciences, 19: 252-2 doi: 1111/j.13652400.2011.00828.x
  • Kohout, J., Šedivá A., Apostolou, A., Stefanov, T., Marić, S., Gaffaroglu, M., and Šlechta, V. 2013. Genetic diversity and phylogenetic origin of brown trout Salmo trutta populations in eastern Balkans. Biologia, Section Zoology, 68(6):1229-1237. doi: 2478/s11756-013-0271-6
  • Kottelat, M. and. Freyhof, J 2007. Handbook of European freshwater fishes. Publications Kottelat, Cornol, Switzerland, 646 pp
  • Kumar, S., Tamura, K., and Nei, M. 2004. MEGA3: Integrated Software for Molecular Evolutionary Genetics Analysis and Sequence Alignment. Briefings in Bioinformatics, 5: 150-163. doi: 1093/bib/5.2.150
  • Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J., and Higgins, D.G. 2007. Clustal W and Clustal X version 2.0. Bioinformatics, 23: 2947-2948. doi: 1093/bioinformatics/btm404
  • Marić, S., Sušnik, S., Simonović, P., Snoj, A. 2006. Phylogeographic study of brown trout from Serbia, based on mitochondrial DNA control region analysis. Genetics Selection Evolution, 38: 411-430. doi:1186/1297-9686-38-4-411
  • Miller, S.A., Dykes, D.D., and Polesky, H.F. 1988. A simple salting out procedure from human nucleated cells. Nucleic Acids Research, 16: 1215.
  • Mrdak, D., Nikolić, V., Tošić A., and Simonović, P. 2012. Molecular and ecological features of the soft-muzzled trout Salmo obtusirostris (Heckel, 1852) in the Zeta river, Montenegro. Biologia, Section Zoology, 67 (1): 222-2 doi: 10.2478/s11756-011-0150-y
  • Simonović, P. Nikolić, V., and Marić, S. 2003. Mid-term fisheries management plan at the catchment of the Timok River]. University of Belgrade, Faculty of Biology, Belgrade, 45 pp. (in Serbian).
  • Simonović, P., Marić, S., Nikolić, V. 2007. Trout Salmo spp. complex in Serbia and adjacent regions of the western Balkans: reconstruction of evolutionary history from external morphology. Journal of Fish Biology, 70 (C) 359–380. doi:10.1111/j.1095862007.01516.x
  • Simonović, P., Nikolić, V., and Tošić, A. 2011. [Fisheries Management Plan for the part of catchment of the Serbia-East fishery area in the 2011-2012 period]. University of Belgrade, Faculty of Biology, Belgrade, 61 pp. (in Serbian)
  • Snoj, A., Jug, T., Melkič, E., Sušnik, S., Pohar, J., Dovč, P., and Budihna, N. 2000. Mitochondrial and microsatellite DNA analysis of marble trout in Slovenia. Journal of Fish Biology, (Quaderni ETP), 29: 5–11.
  • Suárez, J., Bautista, J.M., Almodóvar, A., and Machordom, A. 200 Evolution of mitochondrial control region in Palaeartic brown trout (Salmo trutta) populations: the biogeographical role of the Iberian Peninsula. Heredity, 87: 198–206. doi:1046/j.1365252001.00905.x
  • Sušnik, S., Schöffmann, J., Weiss, S. 2005. Genetic verification of native brown trout from the Persian Gulf (Catak Cay River, Tigris basin). J. Fish Biol., 67: 879–884. doi:10.1016/j.ympev.2006.08.021
  • Swofford, D.L. 2001. PAUP V4.0b10. Sinauer Associates, Inc. Publishers, Sunderland, Massachusetts.
  • Vera, M., Cortey, M., Sanz, N., Garcia-Marin, J.-L. 2010a. Maintenance of an endemic lineage of brown trout (Salmo trutta) within the Duero river basin J Zool Syst Evol Res, 48(2): 181–187. doi: 10.1111/j.1439042009.00547.x
  • Vera, M., Sanz, N., Hansen, M. M., Almodovar, A., GarciaMarin, J.-L. 2010b. Population and family structure of brown trout, Salmo trutta, in a Mediterranean stream. Marine and Freshwater Research, 61: 676–685. doi: 1071/MF09098 1323-1650/10/060676
  • Weiss, S., Antunes, A., Schlötterer, C., Alexandrino, P. 2000. Mitochondrial haplotype diversity among Portuguese brown trout Salmo trutta L. populations: relevance to the post-Pleistocene recolonization of northern Europe. Mol. Ecol., 9: 691–698. doi: 1046/j.1365-294x.2000.00910.x
  • Zhang, D.-X., Hewitt, G.M. 2003. Nuclear DNA analyses in genetic studies of populations: practice, problems and prospects. Molecular Ecology, 12: 563-584. doi: 1046/j.1365-294X.2003.01773.x

New Mitochondrial DNA Haplotype of Brown Trout Salmo trutta l. from Crni Timok Drainage Area in Serbia

Year 2014, Volume: 14 Issue: 1, - , 07.07.2014

Ana Toši63; Dubravka Škraba Vera Nikoli63; Danilo Mrdak Predrag Simonovi63;

https://doi.org/10.4194/1303-2712-v14_1_05

Abstract

Brown trout Salmo trutta wild stocks sustain a remarkable angling and hatchery-reared fish stocking pressure in waters of Serbia, where four drainage-specific and indigenous mitochondrial DNA haplotypes were reported for the drainage area of the Danube River basin. One of these mitochondrial DNA haplotypes, Da23b, was exclusive for brown trout in headwaters of the Crni Timok River (Grand Timok River system, Eastern Serbia). After its discovery in 2003, brown trout stocking was completely halted and abandoned, and a strict Catch-and-Release fishing regime was issued. However, failure of this regime due to enforcement resulted in decline of brown trout fishery. On checking the aboriginality of brood fish sampled in three headwater forks for artificial brown trout propagation, a novel, drainage-specific mtDNA haplotype was recorded in 54% of brown trout. It was assigned Da23c, due to its similarity (substitutions at the two variable sites) to the hitherto known haplotypes of Da23 group. Its finding and absence of Da23b haplotype imply an oversight in earlier haplotype determination. Incorporation of the non-indigenous haplotypes Da2 and Da-s1, introduced in the area by stocking before 2003, into the gene pool of native brown trout and declining of the latter's stock imposes the need for the more stringently enforced conservational activities (genotyping of each brood fish involved in artificial propagation) and improved fisheries management (Catch-and-Release) in the area.

Keywords

D-loop Da23c Eastern Serbia conservation fisheries management

References

  • Apostolidis, A.P., Madeira, M.J., Hansen, M.M., and Machordom, A. 2008. Genetic structure and demographic history of brown trout (Salmo trutta L.) populations from the southern Balkans. Freshwater Biology, 53: 1555–1566. doi: 10.1111/j.1365242008.01988.x
  • Apostolidis, A.P., Stoumboudi, M.Th., Kalogianni, E., Cote, G., Bernatchez, L. 2011. Genetic divergence among native trout Salmo trutta populations from southern Balkans based on mitochondrial DNA and microsatellite variation. Journal of Fish Biology, 79: 1950–1960. doi:10.1111/j.1095-8649.2011.03136.x
  • Avise, J.C. 2000. Phylogeography: the History and Formation of Species. Harvard University Press, Cambridge.
  • Bernatchez, L. 2001. The evolutionary history of brown trout (Salmo trutta L.) inferred from phylogeographic, nested clade, and mismatch analyses of mitochondrial DNA variation. Evolution, 55: 351–379.
  • Bernatchez, L., and Danzmann, R.G. 1993. Congruence in control-region sequence and restriction–site variation in mitochondrial DNA of brook charr (Salvelinus fontinalis Mitchill). Molecular Biology and Evolution, 10: 1002–1014.
  • Bernatchez, L., Guyomard, R., and Bonhomme, F. 1992. DNA sequence variation of the mitochondrial control region among geographically and morphologically remote European brown trout Salmo trutta populations. Molecular Ecology, 1: 161–173.
  • Cortey, M., and García-Marín, J.L. 2002. Evidence for phylogeographically informative sequence variation in the mitochondrial control region of Atlantic brown trout. J. Fish Biol., 60: 1058–1063. doi:1006/jfbi.2002.1910, Duftner, N., Weiss, S., Medgyesy, N., Sturmbauer, C. 2003. Enhanced phylogeographic information about Austrian brown trout populations derived from complete mitochondrial control region sequences. J. Fish Biol., 62: 427–435. doi: 46/j.002212003.00038.x
  • Habibi, E., Kalbassi, M.R., Hosseini S.J., and Qasemi, S.A. 20 Feasibility of Identification of Fall and Spring Migrating Caspian trout (Salmo trutta caspius) by Using AFLP Molecular Marker. Turkish Journal of Fisheries and Aquatic Sciences, 13: 241-248. doi: 4194/1303-2712-v13_2_06
  • Kohout, J. 2012. Effects of stocking on the genetic structure of brown trout, Salmo trutta, in Central Europe inferred from mitochondrial and nuclear DNA markers. Turkish Journal of Fisheries and Aquatic Sciences, 19: 252-2 doi: 1111/j.13652400.2011.00828.x
  • Kohout, J., Šedivá A., Apostolou, A., Stefanov, T., Marić, S., Gaffaroglu, M., and Šlechta, V. 2013. Genetic diversity and phylogenetic origin of brown trout Salmo trutta populations in eastern Balkans. Biologia, Section Zoology, 68(6):1229-1237. doi: 2478/s11756-013-0271-6
  • Kottelat, M. and. Freyhof, J 2007. Handbook of European freshwater fishes. Publications Kottelat, Cornol, Switzerland, 646 pp
  • Kumar, S., Tamura, K., and Nei, M. 2004. MEGA3: Integrated Software for Molecular Evolutionary Genetics Analysis and Sequence Alignment. Briefings in Bioinformatics, 5: 150-163. doi: 1093/bib/5.2.150
  • Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J., and Higgins, D.G. 2007. Clustal W and Clustal X version 2.0. Bioinformatics, 23: 2947-2948. doi: 1093/bioinformatics/btm404
  • Marić, S., Sušnik, S., Simonović, P., Snoj, A. 2006. Phylogeographic study of brown trout from Serbia, based on mitochondrial DNA control region analysis. Genetics Selection Evolution, 38: 411-430. doi:1186/1297-9686-38-4-411
  • Miller, S.A., Dykes, D.D., and Polesky, H.F. 1988. A simple salting out procedure from human nucleated cells. Nucleic Acids Research, 16: 1215.
  • Mrdak, D., Nikolić, V., Tošić A., and Simonović, P. 2012. Molecular and ecological features of the soft-muzzled trout Salmo obtusirostris (Heckel, 1852) in the Zeta river, Montenegro. Biologia, Section Zoology, 67 (1): 222-2 doi: 10.2478/s11756-011-0150-y
  • Simonović, P. Nikolić, V., and Marić, S. 2003. Mid-term fisheries management plan at the catchment of the Timok River]. University of Belgrade, Faculty of Biology, Belgrade, 45 pp. (in Serbian).
  • Simonović, P., Marić, S., Nikolić, V. 2007. Trout Salmo spp. complex in Serbia and adjacent regions of the western Balkans: reconstruction of evolutionary history from external morphology. Journal of Fish Biology, 70 (C) 359–380. doi:10.1111/j.1095862007.01516.x
  • Simonović, P., Nikolić, V., and Tošić, A. 2011. [Fisheries Management Plan for the part of catchment of the Serbia-East fishery area in the 2011-2012 period]. University of Belgrade, Faculty of Biology, Belgrade, 61 pp. (in Serbian)
  • Snoj, A., Jug, T., Melkič, E., Sušnik, S., Pohar, J., Dovč, P., and Budihna, N. 2000. Mitochondrial and microsatellite DNA analysis of marble trout in Slovenia. Journal of Fish Biology, (Quaderni ETP), 29: 5–11.
  • Suárez, J., Bautista, J.M., Almodóvar, A., and Machordom, A. 200 Evolution of mitochondrial control region in Palaeartic brown trout (Salmo trutta) populations: the biogeographical role of the Iberian Peninsula. Heredity, 87: 198–206. doi:1046/j.1365252001.00905.x
  • Sušnik, S., Schöffmann, J., Weiss, S. 2005. Genetic verification of native brown trout from the Persian Gulf (Catak Cay River, Tigris basin). J. Fish Biol., 67: 879–884. doi:10.1016/j.ympev.2006.08.021
  • Swofford, D.L. 2001. PAUP V4.0b10. Sinauer Associates, Inc. Publishers, Sunderland, Massachusetts.
  • Vera, M., Cortey, M., Sanz, N., Garcia-Marin, J.-L. 2010a. Maintenance of an endemic lineage of brown trout (Salmo trutta) within the Duero river basin J Zool Syst Evol Res, 48(2): 181–187. doi: 10.1111/j.1439042009.00547.x
  • Vera, M., Sanz, N., Hansen, M. M., Almodovar, A., GarciaMarin, J.-L. 2010b. Population and family structure of brown trout, Salmo trutta, in a Mediterranean stream. Marine and Freshwater Research, 61: 676–685. doi: 1071/MF09098 1323-1650/10/060676
  • Weiss, S., Antunes, A., Schlötterer, C., Alexandrino, P. 2000. Mitochondrial haplotype diversity among Portuguese brown trout Salmo trutta L. populations: relevance to the post-Pleistocene recolonization of northern Europe. Mol. Ecol., 9: 691–698. doi: 1046/j.1365-294x.2000.00910.x
  • Zhang, D.-X., Hewitt, G.M. 2003. Nuclear DNA analyses in genetic studies of populations: practice, problems and prospects. Molecular Ecology, 12: 563-584. doi: 1046/j.1365-294X.2003.01773.x
There are 27 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Ana Toši63; This is me

Dubravka Škraba This is me

Vera Nikoli63; This is me

Danilo Mrdak This is me

Predrag Simonovi63; This is me

Publication Date July 7, 2014
Published in Issue Year 2014 Volume: 14 Issue: 1

Cite

APA Toši63;, A., Škraba, D., Nikoli63;, V., Mrdak, D., et al. (2014). New Mitochondrial DNA Haplotype of Brown Trout Salmo trutta l. from Crni Timok Drainage Area in Serbia. Turkish Journal of Fisheries and Aquatic Sciences, 14(1). https://doi.org/10.4194/1303-2712-v14_1_05
AMA Toši63; A, Škraba D, Nikoli63; V, Mrdak D, Simonovi63; P. New Mitochondrial DNA Haplotype of Brown Trout Salmo trutta l. from Crni Timok Drainage Area in Serbia. Turkish Journal of Fisheries and Aquatic Sciences. February 2014;14(1). doi:10.4194/1303-2712-v14_1_05
Chicago Toši63;, Ana, Dubravka Škraba, Vera Nikoli63;, Danilo Mrdak, and Predrag Simonovi63;. “New Mitochondrial DNA Haplotype of Brown Trout Salmo Trutta L. From Crni Timok Drainage Area in Serbia”. Turkish Journal of Fisheries and Aquatic Sciences 14, no. 1 (February 2014). https://doi.org/10.4194/1303-2712-v14_1_05.
EndNote Toši63; A, Škraba D, Nikoli63; V, Mrdak D, Simonovi63; P (February 1, 2014) New Mitochondrial DNA Haplotype of Brown Trout Salmo trutta l. from Crni Timok Drainage Area in Serbia. Turkish Journal of Fisheries and Aquatic Sciences 14 1
IEEE A. Toši63;, D. Škraba, V. Nikoli63;, D. Mrdak, and P. Simonovi63;, “New Mitochondrial DNA Haplotype of Brown Trout Salmo trutta l. from Crni Timok Drainage Area in Serbia”, Turkish Journal of Fisheries and Aquatic Sciences, vol. 14, no. 1, 2014, doi: 10.4194/1303-2712-v14_1_05.
ISNAD Toši63;, Ana et al. “New Mitochondrial DNA Haplotype of Brown Trout Salmo Trutta L. From Crni Timok Drainage Area in Serbia”. Turkish Journal of Fisheries and Aquatic Sciences 14/1 (February 2014). https://doi.org/10.4194/1303-2712-v14_1_05.
JAMA Toši63; A, Škraba D, Nikoli63; V, Mrdak D, Simonovi63; P. New Mitochondrial DNA Haplotype of Brown Trout Salmo trutta l. from Crni Timok Drainage Area in Serbia. Turkish Journal of Fisheries and Aquatic Sciences. 2014;14. doi:10.4194/1303-2712-v14_1_05.
MLA Toši63;, Ana et al. “New Mitochondrial DNA Haplotype of Brown Trout Salmo Trutta L. From Crni Timok Drainage Area in Serbia”. Turkish Journal of Fisheries and Aquatic Sciences, vol. 14, no. 1, 2014, doi:10.4194/1303-2712-v14_1_05.
Vancouver Toši63; A, Škraba D, Nikoli63; V, Mrdak D, Simonovi63; P. New Mitochondrial DNA Haplotype of Brown Trout Salmo trutta l. from Crni Timok Drainage Area in Serbia. Turkish Journal of Fisheries and Aquatic Sciences. 2014;14(1).