Effect of High Dietary Carbohydrate on Growth, Serum Physiological Response, and Hepatic Heat Shock Protein 70 Expression of Wuchang Bream (Megalobrama amblycephala) at Two Temperatures

Year 2013, Volume: 13 Issue: 2, - , 01.04.2013

Chuanpeng Zhou Bo Liu Jun Xie Xianping Ge

https://doi.org/10.4194/1303-2712-v13_2_03

Abstract

The effects of high carbohydrate diet on growth, serum physiological response, and hepatic heat shock protein 70 expression in Wuchang bream were determined at 25 °C and 30 °C. The fish were reared at 25 °C had significantly higher serum C3, respiratory burst activity and hepatic superoxide dismutase activity, lower hepatic malonaldehyde than those were reared at 30 °C (P<0.05). At each temperature, the fish fed the control diet (31% CHO) had significantly higher weight gain, specific growth rate, respiratory burst activity, lysozyme, alkaline phosphatase, hepatic total antioxidative capacity, lower feed conversion ratio, glutamic-oxaloacetic transaminase, cortisol, hepatic malonaldehyde and the relative level of hepatic HSP70 mRNA than those fed the high-carbohydrate diet (47% CHO) (P<0.05). Significant interactions between temperature and diet were found for glutamic-oxaloacetic transaminase, C3, lysozyme, alkaline phosphatase and respiratory burst activity and hepatic superoxide dismutase activity (P<0.05). The present study suggested that higher dietary carbohydrate impact growth performance, the nonspecific immune ability and hepatic antioxidant abilities of Wuchang bream at higher temperature.

Keywords

Megalobrama amblycephala; Dietary carbohydrate; Temperature; Growth; Immunity; HSP70

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