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Feasibility of Identification of Fall and Spring Migrating Caspian trout (Salmo trutta caspius) by Using AFLP Molecular Marker

Year 2013, Volume: 13 Issue: 2, - , 01.04.2013

Ensieh Habibi Mohammad Reza Kalbassi Seyed Javad Hosseini Seyed Ahmad Qasemi

https://doi.org/10.4194/1303-2712-v13_2_06

Abstract

Salmo trutta caspius is a valuable endemic species in the Caspian Sea. There are two migrating forms of spring and fall run with different appearance characteristics. AFLP molecular markers were used to evaluate the possibility of identification of fall and spring migrating Caspian trout. 12 different EcoRI/ MseI primer combinations were used, from which a total of 807 bands were produced that 227 of them were polymorphic loci. Percentage of polymorphism loci in spring-run (85.02%) was higher than fall-run (76.21%). The obtained results regarding mean heterozygosity did not show significant differences between fall-run and spring-run Caspian trout. Nei's genetic distance was 0.034% and the variance between and within two populations were 6% and 94%, respectively. The results show that the genetic variation between fall and spring migrating Caspian trout is not significant. Therefore, spring forms are probably a part of Fall-run population and the difference could be attributed to physiology, environment or other related factors.

Keywords

The Caspian Sea Fall-run Genetic variation Spring-run

References

  • Abdoli, A. 2000. The Inland Water Fishes of Iran, 1st ed. Museum of Nature and Wild Life of Iran, Tehran, 378 pp.
  • Allendorf, F.W. and Ryman, N. 1987. Genetic management of hatchery stocks. In: N. Ryman and F. Utter (Eds.), Population Genetics and Fishery Management. University of Washington Press, Seattle: 141–159.
  • Apostolidis, A.P., Madeira, M.J., Hansen, M.M. and Machordom, A. 2008. Genetic structure and demographic history of brown trout (Salmo trutta) populations from the southern Balkans. Freshwater Biology, 53: 1555-1566. doi: 10.1111/j.1365242008.01988.x
  • Baglinière, J.L., Ombredane, D. and Marchand, F. 2000. Critères morphologiques pour l'identification des deux formes (rivière et mer) de truite (Salmo trutta L.) présentes sur un même bassin. Bulletin Francias de la Pêche et de la Pisciculture, 357/358: 375-386.
  • Barannik, V., Borysova, O. and Stolberg, F. 2004. The Caspian Sea region: environmental change. Ambio, 33: 45-51. doi: 10.1579/0044-7447-33.1.45
  • Blears, M.J., de Grandis, S.A., Lee, H. and Trevors, J.T. 19 Amplified fragment length polymorphism (AFLP): A review of the procedure and its applications. Jouranl of Industrial Microbiology and Biotechnology, 21: 99doi: 1038/sj.jim.2900537
  • Bohlin, T., Dellefors, C. and Faremo, U. 1993. Optimal time and size for smolt migration in wild sea trout (Salmo trutta). Canadian Journal of Fisheries and Aquatic Sciences, 50: 224-232. doi: 10.1139/f93-025
  • Bohlin, T., Dellefors, C. and Faremo, U. 1996. Date of smolt migration depends on body-size but not age in wild searun brown trout. Journal of Fish Biology, 49: 157-1 doi: 10.1111/j.1095-8649.1996.tb00012.x
  • Campbell, J.S. 1977. Spawning characteristics of brown trout and sea trout Salmo trutta L. in Kirk Burn, River Tweed, Scotland. Journal of Fish Biology, 11: 2172 doi: 10.1111/j.1095-8649.1977.tb04115.x
  • Charles, K., Guyomard, R., Hoyheim, B., Ombredane, D. and Bagliniere, J.L. 2005. Lack of genetic differentiation between anadromous and resident sympatric brown trout (Salmo trutta) in a Normandy population. Aquatic Living Resources, 18: 65-69. doi: 1051/alr:2005006
  • Coad, B.W. 2000. Criteria for assessing the conservation status of taxa (as applied to Iranian freshwater fishes). Biologia, 55: 537-555.
  • Elo, K., Ivanoff, S., Vuorinen, J.A. and Piironen, J. 1997. Inheritance of RAPD markers and detection of interspecific hybridization with brown trout and Atlantic salmon. Aquaculture, 152: 55-65. doi: 1016/S0044-8486(96)01529-3
  • Englbrecht, C.C., Freyhof, J., Nolte, A., Rassmann, K., Schliewen, U. and Tautz, D. 2000. Phylogeography of the bullhead Cottus gobio (Pisces: Teleostei: Cottidae) suggests a pre-Pleistocene origin of the major central European populations. Molecular Ecology, 9: 7097 doi: 10.1046/j.1365-294x.2000.00912.x
  • Forseth, T., Næsje, T.F., Jonsson, B. and Harsaker, K. 1999. Juvenile migration in brown trout: a consequence of energetic state. Journal of Animal Ecology, 68: 7837 doi: 10.1046/j.1365-2656.1999.00329.x
  • Frost, W.E. and Brown, M.E. 1967. The Trout, Collins.
  • New Naturalist, London, 286 pp. Hansen, M.M., Ruzzante, D.E., Nielsen, E.E. and Mensberg, K.L.D. 2000. Microsatellite and mitochondrial DNA polymorphism reveals lifehistory dependent interbreeding between hatchery and wild brown trout (Salmo trutta L.). Molecular Ecology, 9: 583-5 doi: 1046/j.1365294x.2000.00898.x
  • Hansen, M.M. 2002. Estimating the long-term effects of stocking domesticated trout into wild brown trout (Salmo trutta) populations: an approach using microsatellite DNA analysis of historical and contemporary samples. Molecular Ecology, 11: 100310 doi: 10.1046/j.1365-294X.2002.01495.x
  • Jonsson, B. 1982. Diadromous and resident trout Salmo trutta L.: Is their difference due to genetics? Oikos, 38: 297-300. doi: 10.2307/3544668
  • Jonsson, B. and Jonsson, N. 2001. Polymorphism and speciation in Arctic charr. Journal of Fish Biology, 58: 605-6 doi: 1111/j.1095862001.tb00518.x
  • Kakehi, Y., Nakayama, K., Watanabe, K. and Nishida, M. 200 Inheritance of amplified fragment length polymorphism markers and their utility in population genetic analysis of Plecoglossus altivelis. Journal of Fish Biology, 66: 1529-1544. doi: 10.1111/j.002212005.00683.x
  • Kassam, D., Seki, S., Rusuwa, B., Ambali, A.J.D. and Yamaoka, K. 2005. Genetic diversity within the genus Cynotilapia and its phylogenetic position among Lake Malawi's mbuna cichlids. African Journal of Biotechnology, 4: 1195-120.
  • Kiabi, B.H., Abdoli, A. and Naderi, M. 1999. Status of the fish fauna in the South Caspian basin of Iran. Zoology in the Middle East, 18: 57doi: 1080/09397140.1999.10637782
  • Klemetsen, A., Amundsen, P.A., Dempson, J.B., Jonsson, B., Jonsson, N., O'Connell, M.F. and Mortensen, E. 200 Atlantic salmon Salmo salar L., brown trout Salmo trutta L. and Arctic charr Salvelinus alpinus (L.): a review of aspects of their life histories. Ecology of Freshwater Fish, 12: 1-59. doi: 1034/j.1600-0633.2003.00010.x
  • Liu, Z.J. (ed) 2007. Aquaculture Genome Technologies. Blackwell Publishing, Australia, 551 pp. doi: 1002/9780470277560
  • Liu, Z.J. and Cordes, J.F. 2004. DNA Marker Technologies and their Application in Aquaculture Genetics. Aquaculture, 238: 1-37. doi: 1016/j.aquaculture.2004.05.027
  • Liu, Z.J., Karsi, A., Li, P., Cao, D., Ju, Z. and Dunham, R. 200 An AFLP-based genetic linkage map of channel catfish (Ictalurus punctatus) constructed by using an interspecific hybrid resource family. Genetics, 165: 687-6 Lynch, M. and Milligan, B.G. 1994. Analysis of population genetic structure with RAPD markers. Molecular Ecology, 3: 91doi: 1111/j.1365294X.1994.tb00109.x
  • Money, T., Reader, S., Qu, L.J., Dunford, R.P. and Moore, G. 1996. AFLP based mRNA fingerprinting. Nucleic Acids Research, 24: 2616-2617. doi: 1093/nar/24.13.2616
  • Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89: 583-590.
  • Niksirat, H. and Abdoli, A. 2009. On the status of the critically endangered Caspian brown trout, Salmo trutta caspius, during recent decades in the southern
  • Caspian Sea basin. Zoology in the Middle East, 46: 55- doi: 10.1080/09397140.2009.10638328
  • Nordeng, H. 1983. Solution to the "Char problem" based on Arctic Char (Salvelinus alpinus) in Norway. Canadian Journal of Fisheries and Aquatic Sciences, 40: 137213 doi: 10.1139/f83-159
  • Northcote, T.G. 1997. Potamodromy in Salmonidae: living and moving in the fast lane. North American Journal of Fisheries Management, 17: 1029-1045.
  • Okumuş, I. and Çiftci, Y. 2003. Fish population genetics and molecular markers: II-molecular markers and their applications in fisheries and aquaculture. Turkish Journal of Fisheries and Aquatic Sciences, 3: 51-79.
  • Ostergren, J. 2006. Migration and genetic structure of Salmo salar and Salmo trutta in Northern Swedish rivers. PhD thesis, Uppsala: Swedish University of Agricultural Sciences, 32 pp.
  • Palm, S. and Ryman, N. 1999. Genetic basis of phenotypic differences between transplanted stocks of brown trout. Ecology of Freshwater Fish, 8: 169-180. doi: 1111/j.1600-0633.1999.tb00068.x
  • Rasmussen G. 1986. The population dynamics of brown trout (Salmo trutta L.) in relation to year-class size. Polskie Archiwum Hydrobiologii, 33: 489-508.
  • Sedgwick, S.D. 1995. Trout farming handbook. Alden Press, Oxford.
  • Simmons, M., Mickett, K., Kucuktas, H., Li, P., Dunham, R. and Liu, Z. 2006. Comparison of domestic and wild channel catfish (Ictalurus punctatus) populations provides no evidence for genetic impact. Aquaculture, 252: 133-146. doi: 10.1016/j.aquaculture.2005.11.006 Sivasundar, A. and Hey, J. 2003. Population genetics of Caenorhabditis elegans: the paradox of low polymorphism in a widespread species. Genetics, 163: 147-1
  • Sonstebo, J.H., Borgstrom, R. and Heun, M. 2007. A comparison of AFLPs and microsatellites to identify the population structure of brown trout (Salmo trutta L.) populations from Hardangervidda, Norway. Molecular Ecology, 16: 1427-1438. doi: 1111/j.1365-294X.2007.03256.x
  • Vos, P., Hogers, R., Bleeker, M., Reijans, M., Lee, V., Hornes, T., Frijters, M., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23: 4407-4414. doi: 10.1093/nar/23.21.4407
  • Wang, Z.Y., Tsoi, K.H. and Chu, K.H. 2004. Applications of AFLP technology in genetic and phylogenetic analysis of penaeid shrimp. Biochemical Systematics and Ecology, 32: 399-40 doi: 1016/j.bse.2003.10.006
  • Whitehead, A., Anderson, S.L., Kuivila, K.M., Roach, J.L. and May, B. 2003. Genetic variation among interconnected populations of Catostomus occidentalis: implications for distinguishing impacts of contaminants from biogeographical structuring. Molecular Ecology, 12: 2817-2833. doi: 1046/j.1365-294X.2003.01933.x

Feasibility of Identification of Fall and Spring Migrating Caspian trout (Salmo trutta caspius) by Using AFLP Molecular Marker

Year 2013, Volume: 13 Issue: 2, - , 01.04.2013

Ensieh Habibi Mohammad Reza Kalbassi Seyed Javad Hosseini Seyed Ahmad Qasemi

https://doi.org/10.4194/1303-2712-v13_2_06

Abstract

Salmo trutta caspius is a valuable endemic species in the Caspian Sea. There are two migrating forms of spring and fall run with different appearance characteristics. AFLP molecular markers were used to evaluate the possibility of identification of fall and spring migrating Caspian trout. 12 different EcoRI/ MseI primer combinations were used, from which a total of 807 bands were produced that 227 of them were polymorphic loci. Percentage of polymorphism loci in spring-run (85.02%) was higher than fall-run (76.21%). The obtained results regarding mean heterozygosity did not show significant differences between fall-run and spring-run Caspian trout. Nei's genetic distance was 0.034% and the variance between and within two populations were 6% and 94%, respectively. The results show that the genetic variation between fall and spring migrating Caspian trout is not significant. Therefore, spring forms are probably a part of Fall-run population and the difference could be attributed to physiology, environment or other related factors.

Keywords

The Caspian Sea Fall-run Genetic variation Spring-run

References

  • Abdoli, A. 2000. The Inland Water Fishes of Iran, 1st ed. Museum of Nature and Wild Life of Iran, Tehran, 378 pp.
  • Allendorf, F.W. and Ryman, N. 1987. Genetic management of hatchery stocks. In: N. Ryman and F. Utter (Eds.), Population Genetics and Fishery Management. University of Washington Press, Seattle: 141–159.
  • Apostolidis, A.P., Madeira, M.J., Hansen, M.M. and Machordom, A. 2008. Genetic structure and demographic history of brown trout (Salmo trutta) populations from the southern Balkans. Freshwater Biology, 53: 1555-1566. doi: 10.1111/j.1365242008.01988.x
  • Baglinière, J.L., Ombredane, D. and Marchand, F. 2000. Critères morphologiques pour l'identification des deux formes (rivière et mer) de truite (Salmo trutta L.) présentes sur un même bassin. Bulletin Francias de la Pêche et de la Pisciculture, 357/358: 375-386.
  • Barannik, V., Borysova, O. and Stolberg, F. 2004. The Caspian Sea region: environmental change. Ambio, 33: 45-51. doi: 10.1579/0044-7447-33.1.45
  • Blears, M.J., de Grandis, S.A., Lee, H. and Trevors, J.T. 19 Amplified fragment length polymorphism (AFLP): A review of the procedure and its applications. Jouranl of Industrial Microbiology and Biotechnology, 21: 99doi: 1038/sj.jim.2900537
  • Bohlin, T., Dellefors, C. and Faremo, U. 1993. Optimal time and size for smolt migration in wild sea trout (Salmo trutta). Canadian Journal of Fisheries and Aquatic Sciences, 50: 224-232. doi: 10.1139/f93-025
  • Bohlin, T., Dellefors, C. and Faremo, U. 1996. Date of smolt migration depends on body-size but not age in wild searun brown trout. Journal of Fish Biology, 49: 157-1 doi: 10.1111/j.1095-8649.1996.tb00012.x
  • Campbell, J.S. 1977. Spawning characteristics of brown trout and sea trout Salmo trutta L. in Kirk Burn, River Tweed, Scotland. Journal of Fish Biology, 11: 2172 doi: 10.1111/j.1095-8649.1977.tb04115.x
  • Charles, K., Guyomard, R., Hoyheim, B., Ombredane, D. and Bagliniere, J.L. 2005. Lack of genetic differentiation between anadromous and resident sympatric brown trout (Salmo trutta) in a Normandy population. Aquatic Living Resources, 18: 65-69. doi: 1051/alr:2005006
  • Coad, B.W. 2000. Criteria for assessing the conservation status of taxa (as applied to Iranian freshwater fishes). Biologia, 55: 537-555.
  • Elo, K., Ivanoff, S., Vuorinen, J.A. and Piironen, J. 1997. Inheritance of RAPD markers and detection of interspecific hybridization with brown trout and Atlantic salmon. Aquaculture, 152: 55-65. doi: 1016/S0044-8486(96)01529-3
  • Englbrecht, C.C., Freyhof, J., Nolte, A., Rassmann, K., Schliewen, U. and Tautz, D. 2000. Phylogeography of the bullhead Cottus gobio (Pisces: Teleostei: Cottidae) suggests a pre-Pleistocene origin of the major central European populations. Molecular Ecology, 9: 7097 doi: 10.1046/j.1365-294x.2000.00912.x
  • Forseth, T., Næsje, T.F., Jonsson, B. and Harsaker, K. 1999. Juvenile migration in brown trout: a consequence of energetic state. Journal of Animal Ecology, 68: 7837 doi: 10.1046/j.1365-2656.1999.00329.x
  • Frost, W.E. and Brown, M.E. 1967. The Trout, Collins.
  • New Naturalist, London, 286 pp. Hansen, M.M., Ruzzante, D.E., Nielsen, E.E. and Mensberg, K.L.D. 2000. Microsatellite and mitochondrial DNA polymorphism reveals lifehistory dependent interbreeding between hatchery and wild brown trout (Salmo trutta L.). Molecular Ecology, 9: 583-5 doi: 1046/j.1365294x.2000.00898.x
  • Hansen, M.M. 2002. Estimating the long-term effects of stocking domesticated trout into wild brown trout (Salmo trutta) populations: an approach using microsatellite DNA analysis of historical and contemporary samples. Molecular Ecology, 11: 100310 doi: 10.1046/j.1365-294X.2002.01495.x
  • Jonsson, B. 1982. Diadromous and resident trout Salmo trutta L.: Is their difference due to genetics? Oikos, 38: 297-300. doi: 10.2307/3544668
  • Jonsson, B. and Jonsson, N. 2001. Polymorphism and speciation in Arctic charr. Journal of Fish Biology, 58: 605-6 doi: 1111/j.1095862001.tb00518.x
  • Kakehi, Y., Nakayama, K., Watanabe, K. and Nishida, M. 200 Inheritance of amplified fragment length polymorphism markers and their utility in population genetic analysis of Plecoglossus altivelis. Journal of Fish Biology, 66: 1529-1544. doi: 10.1111/j.002212005.00683.x
  • Kassam, D., Seki, S., Rusuwa, B., Ambali, A.J.D. and Yamaoka, K. 2005. Genetic diversity within the genus Cynotilapia and its phylogenetic position among Lake Malawi's mbuna cichlids. African Journal of Biotechnology, 4: 1195-120.
  • Kiabi, B.H., Abdoli, A. and Naderi, M. 1999. Status of the fish fauna in the South Caspian basin of Iran. Zoology in the Middle East, 18: 57doi: 1080/09397140.1999.10637782
  • Klemetsen, A., Amundsen, P.A., Dempson, J.B., Jonsson, B., Jonsson, N., O'Connell, M.F. and Mortensen, E. 200 Atlantic salmon Salmo salar L., brown trout Salmo trutta L. and Arctic charr Salvelinus alpinus (L.): a review of aspects of their life histories. Ecology of Freshwater Fish, 12: 1-59. doi: 1034/j.1600-0633.2003.00010.x
  • Liu, Z.J. (ed) 2007. Aquaculture Genome Technologies. Blackwell Publishing, Australia, 551 pp. doi: 1002/9780470277560
  • Liu, Z.J. and Cordes, J.F. 2004. DNA Marker Technologies and their Application in Aquaculture Genetics. Aquaculture, 238: 1-37. doi: 1016/j.aquaculture.2004.05.027
  • Liu, Z.J., Karsi, A., Li, P., Cao, D., Ju, Z. and Dunham, R. 200 An AFLP-based genetic linkage map of channel catfish (Ictalurus punctatus) constructed by using an interspecific hybrid resource family. Genetics, 165: 687-6 Lynch, M. and Milligan, B.G. 1994. Analysis of population genetic structure with RAPD markers. Molecular Ecology, 3: 91doi: 1111/j.1365294X.1994.tb00109.x
  • Money, T., Reader, S., Qu, L.J., Dunford, R.P. and Moore, G. 1996. AFLP based mRNA fingerprinting. Nucleic Acids Research, 24: 2616-2617. doi: 1093/nar/24.13.2616
  • Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89: 583-590.
  • Niksirat, H. and Abdoli, A. 2009. On the status of the critically endangered Caspian brown trout, Salmo trutta caspius, during recent decades in the southern
  • Caspian Sea basin. Zoology in the Middle East, 46: 55- doi: 10.1080/09397140.2009.10638328
  • Nordeng, H. 1983. Solution to the "Char problem" based on Arctic Char (Salvelinus alpinus) in Norway. Canadian Journal of Fisheries and Aquatic Sciences, 40: 137213 doi: 10.1139/f83-159
  • Northcote, T.G. 1997. Potamodromy in Salmonidae: living and moving in the fast lane. North American Journal of Fisheries Management, 17: 1029-1045.
  • Okumuş, I. and Çiftci, Y. 2003. Fish population genetics and molecular markers: II-molecular markers and their applications in fisheries and aquaculture. Turkish Journal of Fisheries and Aquatic Sciences, 3: 51-79.
  • Ostergren, J. 2006. Migration and genetic structure of Salmo salar and Salmo trutta in Northern Swedish rivers. PhD thesis, Uppsala: Swedish University of Agricultural Sciences, 32 pp.
  • Palm, S. and Ryman, N. 1999. Genetic basis of phenotypic differences between transplanted stocks of brown trout. Ecology of Freshwater Fish, 8: 169-180. doi: 1111/j.1600-0633.1999.tb00068.x
  • Rasmussen G. 1986. The population dynamics of brown trout (Salmo trutta L.) in relation to year-class size. Polskie Archiwum Hydrobiologii, 33: 489-508.
  • Sedgwick, S.D. 1995. Trout farming handbook. Alden Press, Oxford.
  • Simmons, M., Mickett, K., Kucuktas, H., Li, P., Dunham, R. and Liu, Z. 2006. Comparison of domestic and wild channel catfish (Ictalurus punctatus) populations provides no evidence for genetic impact. Aquaculture, 252: 133-146. doi: 10.1016/j.aquaculture.2005.11.006 Sivasundar, A. and Hey, J. 2003. Population genetics of Caenorhabditis elegans: the paradox of low polymorphism in a widespread species. Genetics, 163: 147-1
  • Sonstebo, J.H., Borgstrom, R. and Heun, M. 2007. A comparison of AFLPs and microsatellites to identify the population structure of brown trout (Salmo trutta L.) populations from Hardangervidda, Norway. Molecular Ecology, 16: 1427-1438. doi: 1111/j.1365-294X.2007.03256.x
  • Vos, P., Hogers, R., Bleeker, M., Reijans, M., Lee, V., Hornes, T., Frijters, M., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23: 4407-4414. doi: 10.1093/nar/23.21.4407
  • Wang, Z.Y., Tsoi, K.H. and Chu, K.H. 2004. Applications of AFLP technology in genetic and phylogenetic analysis of penaeid shrimp. Biochemical Systematics and Ecology, 32: 399-40 doi: 1016/j.bse.2003.10.006
  • Whitehead, A., Anderson, S.L., Kuivila, K.M., Roach, J.L. and May, B. 2003. Genetic variation among interconnected populations of Catostomus occidentalis: implications for distinguishing impacts of contaminants from biogeographical structuring. Molecular Ecology, 12: 2817-2833. doi: 1046/j.1365-294X.2003.01933.x
There are 42 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Ensieh Habibi This is me

Mohammad Reza Kalbassi This is me

Seyed Javad Hosseini This is me

Seyed Ahmad Qasemi This is me

Publication Date April 1, 2013
Published in Issue Year 2013 Volume: 13 Issue: 2

Cite

APA Habibi, E., Kalbassi, M. R., Hosseini, S. J., Qasemi, S. A. (2013). Feasibility of Identification of Fall and Spring Migrating Caspian trout (Salmo trutta caspius) by Using AFLP Molecular Marker. Turkish Journal of Fisheries and Aquatic Sciences, 13(2). https://doi.org/10.4194/1303-2712-v13_2_06
AMA Habibi E, Kalbassi MR, Hosseini SJ, Qasemi SA. Feasibility of Identification of Fall and Spring Migrating Caspian trout (Salmo trutta caspius) by Using AFLP Molecular Marker. Turkish Journal of Fisheries and Aquatic Sciences. April 2013;13(2). doi:10.4194/1303-2712-v13_2_06
Chicago Habibi, Ensieh, Mohammad Reza Kalbassi, Seyed Javad Hosseini, and Seyed Ahmad Qasemi. “Feasibility of Identification of Fall and Spring Migrating Caspian Trout (Salmo Trutta Caspius) by Using AFLP Molecular Marker”. Turkish Journal of Fisheries and Aquatic Sciences 13, no. 2 (April 2013). https://doi.org/10.4194/1303-2712-v13_2_06.
EndNote Habibi E, Kalbassi MR, Hosseini SJ, Qasemi SA (April 1, 2013) Feasibility of Identification of Fall and Spring Migrating Caspian trout (Salmo trutta caspius) by Using AFLP Molecular Marker. Turkish Journal of Fisheries and Aquatic Sciences 13 2
IEEE E. Habibi, M. R. Kalbassi, S. J. Hosseini, and S. A. Qasemi, “Feasibility of Identification of Fall and Spring Migrating Caspian trout (Salmo trutta caspius) by Using AFLP Molecular Marker”, Turkish Journal of Fisheries and Aquatic Sciences, vol. 13, no. 2, 2013, doi: 10.4194/1303-2712-v13_2_06.
ISNAD Habibi, Ensieh et al. “Feasibility of Identification of Fall and Spring Migrating Caspian Trout (Salmo Trutta Caspius) by Using AFLP Molecular Marker”. Turkish Journal of Fisheries and Aquatic Sciences 13/2 (April 2013). https://doi.org/10.4194/1303-2712-v13_2_06.
JAMA Habibi E, Kalbassi MR, Hosseini SJ, Qasemi SA. Feasibility of Identification of Fall and Spring Migrating Caspian trout (Salmo trutta caspius) by Using AFLP Molecular Marker. Turkish Journal of Fisheries and Aquatic Sciences. 2013;13. doi:10.4194/1303-2712-v13_2_06.
MLA Habibi, Ensieh et al. “Feasibility of Identification of Fall and Spring Migrating Caspian Trout (Salmo Trutta Caspius) by Using AFLP Molecular Marker”. Turkish Journal of Fisheries and Aquatic Sciences, vol. 13, no. 2, 2013, doi:10.4194/1303-2712-v13_2_06.
Vancouver Habibi E, Kalbassi MR, Hosseini SJ, Qasemi SA. Feasibility of Identification of Fall and Spring Migrating Caspian trout (Salmo trutta caspius) by Using AFLP Molecular Marker. Turkish Journal of Fisheries and Aquatic Sciences. 2013;13(2).