Effects of Stocking Density on Blood Cortisol, Glucose and Cholesterol Levels of Immature Siberian Sturgeon (Acipenser baerii Brandt, 1869)

Year 2013, Volume: 13 Issue: 1, - , 01.02.2013

Alireza Hasanalipour Soheil Eagderi Hadi Poorbagher Mahmoud Bahmani

https://doi.org/10.4194/1303-2712-v13_1_04

Abstract

Siberian sturgeon (Acipenser baerii Brandt, 1869) is a suitable species for aquacultural purposes. This study was conducted to investigate the influence of stocking density on blood parameters of cultured immature Siberian sturgeon introduced to Iranian aquaculture systems, in order to monitor crowding stress. Two-year old juvenile fish (weight 607.7±21.49 g, total length: 68±9.46 cm [mean ± SE]) were reared at low, moderate and high densities (6, 12 and 18 fish 250 L-1, respectively) for 5 months. Cortisol, glucose and cholesterol concentration of blood plasma were measured four times during the experiment (July, August, October and November 2008). The present study indicated that (1) density had no significant effect of blood cortisol, glucose and cholesterol concentration in A. baerii (cortisol: 7.8-13.1, cholesterol: 115.6-133.9, glucose: 41.4-46.8 ng ml-1[range]) (2) a significant increase in cortisol concentration of all densities was found in August which was related to water temperature rise (23.75±2.69 in August vs 7.09±1.16 ng ml-1 for other months [mean ± SE]). Therefore, it seems that the Siberian sturgeon is not sensitive species against rearing density in aquaculture systems. Further investigation on critical density and factors that change tolerability to high density are needed to be addressed.

Keywords

Siberian sturgeon, stress, rearing density, growth

References

• Asadi, F., Hallajian, A., Asadian, P., Shahriari, A. and Pourkabir, M. 2009. Serum lipid, free fatty acid, and proteins in juvenile sturgeons: Acipenser persicus and Acipenser stellatus. Comparative Clinical Pathology, 18: 287-289. doi: 10.1007/s00580-008-0797-0;
• Bartley, D.M. and Rana, K. 1998. Stocking inland waters of the Islamic Republic of Iran. The FAO Aquaculture Newsletter, 18: 16-19.
• Barton, B.A. 2000. Salmonid fishes differ in their cortisol and glucose responses to handling and transport stress. North American Journal of Aquaculture, 62: 12 doi: 10.1577/1548-8454(2000)062;
• Barton, B.A. 2002. Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteroids. Integrative and Comparative Biology, 42: 517-525. doi: 10.1093/icb/42.3.517;
• Barton, B.A., Rahn, A.B., Feist, G., Bollig, H. and Schreck, C.B. 1998. Physiological stress responses of the freshwater chondrostean paddlefish (Polyodon spathula) to acute physical disturbances. Comparative Biochemistry Physiology, 120(A): 355-363.
• Biswas, A.K., Seoka, M., Tanaka, Y., Takii, K. and Kumai, H. 2006. Effect of photoperiod manipulation on the growth performance and stress response of juvenile red sea bream (Pagrus major). Aquaculture, 258: 3503 doi: 10.1016/j.aquaculture.2006.03.048;
• Bolasina, S., Tagawa, M., Yamashita, Y. and Tanaka, M. 200 Effect of stocking density on growth, digestive enzyme activity and cortisol level in larvae and juveniles of Japanese flounder, Paralichthys olivaceus. Aquaculture, 259: 432-443. Cataldi, E., Di Marco, P., Mandich, A. and Cataudella, S. 19 Serum parameters of Adriatic sturgeon Acipenser naccarii (Pisces: Acipenseriformes): effects of temperature and stress. Comparative Biochemistry and Physiology, 121(A): 351-354. Cech, J., Mitchell, S. and Wragg, T. 1984. Comparative growth of juvenile white sturgeon and striped bass: effects of temperature and hypoxia. Estuaries and Coasts, 7: 12-18.
• Doroshov, S.I., Moberg, G.P. and Eenennaam, J.P.V. 1997. Observations on the reproductive cycle of cultured white sturgeon, Acipenser transmontanus. Journal of Fish Biology, 48: 265–278. doi: 1007/0-306-46854-9_16
• Doukakis, P., Birstein, V.J., Ruban, G.I. and DeSalle, R. 19 Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A. stellatus. Molecular Ecology, 8: 117-127. doi: 1046/j.1365-294x.1999.00816.x;
• Falahatkar, B., Poursaeid, S., Shakoorian, M. and Barton, B. 200 Responses to handling and confinement stressors in juvenile great sturgeon Huso huso. Journal of Fish Biology, 75: 784–796. doi:1111/j.1095-8649.2009.02334.x.
• Gessner, J., Horvath, A., Arndt, G.M., Urbányi, B., Anders, E., Hegyi, A. and Wuertz, S. 2009. Intercontinental transfer of adult Acipenser oxyrinchus impact assessment of aviation transport conditions on blood parameters. Journal of Applied Ichthyology, 25: 3653 doi: 10.1111/j.1439-0426.2009.01188.x.
• Ghomi, M.R., Nazari, R.M., Poorbagher, H., Sohrabnejad, M., Jamalzadeh, H.R., Ovissipour, M., Molla, A.E. and Zarei, M. 2010. Effect of photoperiod on blood parameters of young beluga sturgeon (Huso huso Linnaeus, 1758). Comparative Clinical Pathology, 20: 647-6 doi: 10.1007/s00580-010-1051-0;
• Glass, G.V. 1966. Testing Homogeneity of Variances. American Educational Research Journal, 3: 187-190. doi: 1037/0033-2909.86.5.978.
• Hallaraker, H., Folkvord, A. and Stefansson, S.O. 1995. Growth of juvenile halibut (Hippoglossus hippoglossus) related to temperature, day length and feeding regime. Netherlands Journal of Sea Research, 34: 139-147. doi: 10.1016/0077-7579(95)90022-5.
• Holcik, J. 1989. The freshwater fishes of Europe. Aulaverlag Wiesbaden, 1: 227-262.
• Iguchi, K., Ogawa, K., Nagae, M. and Ito, F. 2003. The influence of rearing density on stress response and disease susceptibility of ayu (Plecoglossus altivelis). Aquaculture, 220: 515-523. doi: 10.1016/S0044-8486 (02)00626-9.
• Jarvis, P.L., Ballantyne, J.S. and Hogans, W.E. 2001. The Influence of Salinity on the Growth of Juvenile Shortnose Sturgeon. North American Journal of Aquaculture, 63: 272-276. doi: 10.1577/1548-8454.
• Jodun, W.A., Millard, M.J. and Mohler, J. 2002. The Effect of Rearing Density on Growth, Survival, and Feed Conversion of Juvenile Atlantic Sturgeon. North American Journal of Aquaculture, 64: 10-15.
• Koksal, G., Rad, F. and Kindir, M. 2000. Growth performance and feed conversion efficiency of Siberian sturgeon (Acipenser baerii) juvenile reared in concrete raceways. Turkish Journal of Veterinary and Animal Science, 24: 435-442.
• Kottelat, M. and Freyhof, J. 2007. Handbook of European freshwater fishes. Publications Kottelat. Cornol, Switzerland.
• Lankford, S.E., Adams, T.E. and Cech, J.J. 2003. Time of day and water temperature modify the physiological stress response in green sturgeon, Acipenser medirostris Comparative Biochemistry and Physiology, 135(A): 291-302. doi: 10.1016/S10956433(03)00075-8;
• Little, T.M. and Hills, F. 19 Agricultural experimentation: design and analysis. John Wiley, New York, 368 pp. Marino, G., Di Marco, P., Mandich, A., Finoia, M.G. and Cataudella, S. 2001. Changes in serum cortisol, metabolites, osmotic pressure and electrolytes in response to different blood sampling procedures in cultured sea bass (Dicentrarchus labrax L.). Journal of Applied Ichthyology, 17: 115-120. doi: 1111/j.1439-0426.2001.00284.x;
• Maxime, V., Nonnotte, G., Peyraud, C., Williot, P. and Truchot, J.P. 1995. Circulatory and respiratory effects of an hypoxic stress in the Siberian sturgeon. Respiration Physiology, 100: 203-212. doi: 1016/0034-5687(95)00003-V.
• North, B.P., Turnbull, J.F., Ellis, T., Porter, M.J., Migaud, H., Bron, J. and Bromage, N.R. 2006. The impact of stocking density on the welfare of rainbow trout (Oncorhynchus mykiss). Aquaculture, 255: 466-479. doi: 1016/j.aquaculture.2006.01.004;
• Øverli, Ø., Olsen, R.E., Lİvik, F. and Ringİ, E. 1999. Dominance hierarchies in Arctic charr, Salvelinus alpinus L.: differential cortisol profiles of dominant and subordinate individuals after handling stress. Aquaculture Research, 30: 259-264.
• Papoutsoglou, S.E., Karakatsouli, N., Louizos, E., Chadio, S., Kalogiannis, D., Dalla, C., Polissidis, A. and Papadopoulou-Daifoti, Z. 2007. Effect of Mozart's music (Romanze-Andante of "Eine Kleine Nacht Musik", sol major, K525) stimulus on common carp (Cyprinus carpio L.) physiology under different light conditions. Aquacultural Engineering, 36: 61-72. doi: 1016/j.aquaeng.2006.07.001;
• Pickering, A.D. and Pottinger, T.G. 1987a. Crowding causes prolonged leucopenia in salmonid fish, despite interrenal acclimation. Journal of Fish Biology, 30: 701-7 doi: 10.1111/j.1095-8649.1987.tb05799.x.
• Pickering, A.D. and Pottinger, T.G. 1987b. Poor water quality suppresses the cortisol response of salmonid fish to handling and confinement. Journal of Fish Biology, 30: 363-374. doi: 10.1111/j.1095-8649. 19tb05761.x.
• Pickering, A.D. and Stewart, A. 1984. Acclimation of the interrenal tissue of the brown trout, Salmo trutta L., to chronic crowding stress. Journal of Fish Biology, 24: 731-7 doi: 10.1111/j.1095-8649.1984.tb04844.x;
• Quinn, G.P. and Keough, M. J. 2002. Experimental design and data analysis for biologists. Cambridge University Press. Cambridge, 537 pp.
• Rafatnezhad, S., Falahatkar, B. and Gilani, M.H.T. 2008. Effects of stocking density on haematological parameters, growth and fin erosion of great sturgeon (Huso huso) juveniles. Aquaculture Research, 39: 1506-1513. doi: 10.1111/j.1365-2109.2008.02020.x;
• Ramsay, J.M., Feist, G.W., Varga, Z.M., Westerfield, M., Kent, M.L. and Schreck, C.B. 2006. Whole-body cortisol is an indicator of crowding stress in adult zebrafish, Danio rerio. Aquaculture, 258: 565-574. doi: 1016/j.aquaculture.2006.04.020;
• Rotllant, J., Balm, P.H.M., Perez-Sanchez, J., WendelaarBonga, S.E. and Tort, L. 2001. Pituitary and interrenal function in gilthead sea bream (Sparus aurata L., Teleostei) after handling and confinement stress. General and Comparative Endocrinology, 121: 3333 doi: 10.1006/gcen.2001.7604;
• Ruchin, A. 2007. Effect of photoperiod on growth, physiologica and hematological indices of juvenile Siberian sturgeon Acipenser baerii. Biology Bulletin, 34: 583-589.
• Shahsavani, D., Kazerani, H., Kaveh, S. and GholipourKanani, H. 2010. Determination of some normal serum parameters in starry sturgeon (Acipenser stellatus Pallas, 1771) during spring season. Comparative Clinical Pathology, 19: 57-61. doi: 1007/s00580-009-0899-3;
• Trenzado, C.E., Carrick, T.R. and Pottinger, T.G. 2003. Divergence of endocrine and metabolic responses to stress in two rainbow trout lines selected for differing cortisol responsiveness to stress. General and Comparative Endocrinology, 133: 332-340. doi: 1016/S0016-6480(03)00191-6;
• Underwood, A.J. 1997. Experiments in ecology: their logical design and interpretation using analysis of variance. Cambridge University Press. Cambridge.
• Valenzuela, A., Silva, V. and Klempau, A. 2008. Effects of different artificial photoperiods and temperatures on haematological parameters of rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 34: 159-167.
• Wedemeyer, G.A., Barton, B.A. and McLeay, D.J. 1990. Stress and acclimation. In: C.B. Schreck, P.B. Moyle (Eds.), Methods for fish biology. American Fisheries
• Society Bethesda, Maryland: 451-489. Weil, L.S., Barry, T.P. and Malison, J.A. 2001. Fast growth in rainbow trout is correlated with a rapid decrease in post-stress cortisol concentrations. Aquaculture, 193: 373-3 doi: 10.1016/S0044-8486(00)00482-8;
• Yousefi, M., Abtahi, B. and Kenari, A. 2011. Hematological, serum biochemical parameters, and physiological responses to acute stress of Beluga sturgeon (Huso huso, Linnaeus 1785) juveniles fed dietary nucleotide. Comparative Clinical Pathology, 1- doi: 10.1007/s00580-011-1225-4;
• Yousif, O.M. 2002. The effects of stocking density, water exchange rate, feeding frequency and grading on size hierarchy development in juvenile Nile tilapia, Oreochromis niloticus L. Emirates Journal of Agricultural Sciences, 14: 45-53.
• Zar, J. H. 1999. Biostatistical Analysis, 4th. Prentice-Hall, New Jersey, 929 pp.
• Zarejabad, A.M., Sudagar, M., Pouralimotlagh, S. and Bastami, K.D. 2009. Effects of rearing temperature on hematological and biochemical parameters of great sturgeon (Huso huso Linnaeus, 1758) juvenile. Comparative Clinical Pathology, 19: 367-371. doi: 1007/s00580-009-0880-1