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Effect of Synthetic Luteinizing Hormone - Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus frisii kutum

Year 2013, Volume: 13 Issue: 1, - , 01.02.2013

Mohaddeseh Ahmadnezhad Shahrbanoo Oryan Homayoun Hosseinzadeh Sahafi Hossein Khara

https://doi.org/10.4194/1303-2712-v13_1_12

Abstract

To assess changes of gonad development and sex steroids hormones of R. frisii kutum, broodfish were captured during spawning season 2010 (March-April) and induced intramuscularly with following hormone treatments: single injections of 1 μg kg-1 b.w. LHRH-A2, combination of LHRH-A2 with two dopamine antagonists as follows: 1 μg kg-1 b.w. LHRH-A2 + 5 mg kg-1 b.w. PIM, 1 μg kg-1 b.w. LHRH-A2 + 2.5 mg kg-1 b.w. chlorpromazine, 1 μg kg-1 b.w. LHRH-A2 + 5 mg kg-1 b.w. PIM + 2.5 mg kg-1 b.w. chlorpromazine, single injections of 5 mg kg-1 b.w. PIM and 2.5 mg kg-1 b.w. chlorpromazine, carp pitatury extract (CPE), saline solution (NaCl 0.7%) and control group (without injection) were used, respectively. The number of ovulated fish was different in each treatment. Ovaries were more progressed in ovulated rather than non-ovulated fish. Also, sex hormones such as testosterone (T) and estradiol (E2) were changed in different hormonal treatments. In ovulated fish, histological analysis revealed six stages in ovaries, while ovaries in non-ovulated ones were developed only in fourth stage

Keywords

Spawning season Histology Steroid profiles Rutilus frisii kutum

References

  • Chang, J.P. and Peter, R.E. 1983. Effects of pimozide and des Gly’O [o-Ala61 luteinizing hormone- releasing hormone ethylamide on serum gonadotropin concentrations, germinal vesicle migration and ovulation in female goldfish, Carassius auratus. General and Comparative Endocrinology, 52: 30-37.
  • Chang, J., Peter, R.E., Nahorniak, C.S. and Sokolowska, M. 1984. Effects of catecholaminergic agonists and antagonists on serum gonadotropin concentrations and ovulation in goldfish: Evidence for specificity of dopamine inhibition of gonadotropin secretion. General and Comparative Endocrinology, 55: 3513
  • Dahle, R., Taranger, G.L., Karlsen, O., Kjesbu, O.S. and Norberg, B. 2003. Gonadal development and associated changes in liver size and sexual steroids during the reproductive cycle of captive male and female Atlantic cod (Gadus morhua L.). Comparative Biochemistry and Physiology, 136: 641-653.
  • De Leeuw, R., Resink, J.W., Rooyakkers, E.J.M. and Goos, H.J. 1985a. Pimozide modulates the luteinizing hormone-releasing hormone effect on gonadotropin release in the African catfish, Clarias lazera. General and Comparative Endocrinology, 58: 120-127.
  • De Leeuw, R., Goos, H.J., Richter, C.J.J. and Eding, E.H. 1985b. Pimozide-LHRHa induced breeding of the African catfish, Clarias gariepinus (Burchell). Aquaculture, 44: 295-302.
  • Emadi, H. 1995. Rutilus frissi kutum is being victim of management problem, rapacity and tradition. J Aquatic, 3: 10-12.
  • Fostier, A., Jalabert, B., Billard, R., Breton, B. and Zohar, Y. 1983. The gonadal steroids. In: WS Hoar, DJ, Randall, Donaldson EM (Eds). Fish Physiology. (9A). Academic Press, New York.
  • Gothilf, Y., Meiri, I., Elizur, A. and Zohar, Y. 1997. Preovulatory changes in the levels of three gonadotropin-releasing hormone-encoding messenger ribonucleic acids (mRNAs), gonadotropin betasubunit mRNAs, plasma gonadotropin, and steroids in the female gilthead seabream, Sparus aurata. Biology of Reproduction, 5: 1145-54.
  • Kesteven, G.L. 1960. Manual of Field Methods in Fisheries Biology. F.A.O. Manual fish science, 1: 1-52.
  • Kobayashi, M., Aida, K. and Hanyu, U. 1987. Hormone changes during ovulation and effects of steroid hormones on plasma gonadotropin levels and ovulation in goldfish. General and Comparative Endocrinology, 67: 24-32.
  • Kozul, V., Scaramuca, B., Glamuzina, B., Glavic, N. and Tutman, P. 2001. Comparative gonadogenesis and hormonal induction of spawning of cultured and wild Mediterranean amberjack (Seriola dumerili, Risso 1810). Scientia Marina, 65: 215-220.
  • Lamba, V., Goswami, S.V. and Sundararaj, B.I. 1983. Circannual and circadian variations in plasma levels of steroids (Cortisol, estradiol-17b, estrone, and testosterone) correlated with the annual gonadal cycle in the catfish, Heteropneustes fossilis (Bloch). General and Comparative Endocrinology, 50: 2052
  • Levavi-Zermonsky, B. and Yaron, Z. 1986. Changes in gonadotropin and ovarian- steroids associated with oocytes maturation during spawning induction in the carp. General and Comparative Endocrinology, 62: 89Lokman, P.M., Vermeulen, G.J., Lambert, J.G.D. and Young, G. 1998. Gonad histology and plasma steroid profiles in wild New Zealand freshwater eels (Anguilla dieffenbachii and A. australis) before and at the onset of the natural spawning migration. Fish Physiology and Biochemistry, 19: 325–338.
  • Malison, J.A., Procarione, T.P., Barry, A, Kapuscinski, A.R. and Kayes, T.B. 1994. Endocrine and gonadal changes during the annual reproductive cycle of the freshwater teleost, Stizostedion virteum. Fish 100 Physiology and Biochemistry, 13: 473-484. Matsuyama, M.S., Adachi, Y., Nagahama, K. and Maruyama, S. 1990. Diurnal rhythm of serum steroid hormone levels in the Japanese whiting, Sillago japonica, a daily spawning teleost. Fish Physiology and Biochemistry, 8: 329-338. Matsuyama, M., Adachi, S., Nagahama, Y., Kitajima, C. and Matsuura, S. 1991. Annual reproductive cycle of the captive female Japanese sardine Sardinops melanostictus: relationship to ovarian development and serum levels of gonadal steroid hormones. Marine Biology, 108: 21-29. Nagahama, Y. 1987. Gonadotropin action on gametogenesis and steroidogenesis in Teleost gonads. Zoological Science, 4: 209-222. Nagahama, Y. 1994. Endocrine regulation of gametogenesis in fish. International Journal of Developmental Biology, 38: 217-229. Pankhurst, N.W., Stacey, N.E. and Van Der Kraak, G. 1986. Reproductive development and plasma levels of reproductive hormones of goldeye, Hiodon alosoides (Rafinesque), taken from the North Saskatchewan River during the open-water season. Canadian Journal of Zoology, 64: 2843–2849. Pankhurst, N.W. and Conroy, A.M. 1988. Endocrine changes during gonadal maturation and spawning in the orange roughy (Hoplostethus atlanticus Colett), a teleost from the midslope waters off New Zealand. General and Comparative Endocrinology, 70: 262– 2 Persson, P., Sundell, K., Bjornsson, B.T.H. and Lundovist, H. 1998. Calcium metabolism and osmoregulation during sexual Maturation of river running Atlantic salmon. Journal of Fish Biology, 52: 334-349. Peter, R.E., Crim, L.W., Goos, H.J.T. and Grim, J.W. 1978. Lesioning studies on the gravid female goldfish: Neuroendocrine regulation of ovulation. General and Comparative Endocrinology, 35: 391-401 Pham, H.Q., Nguyen, A.T., Nguyen, M.D. and Arukwe, A. 20 Sex steroid levels, oocyte maturation and spawning performance in Waigieu sea perch (Psammoperca waigiensis) exposed to thyroxin, human chorionic gonadotropin, luteinizing hormone releasing hormone and carp pituitary extract. Comparative Biochemistry and Physiology, 155: 2232
  • Planas, J.V., Athos, J., Goetz, F.W. and Swanson, P. 2000. Regulation of ovarian steroidogenesis in vitro by follicle-stimulating hormone and luteinizing hormone during sexual maturation in salmonid fish. Biology of Reproduction, 62: 1262-1269.
  • Rinchard, J., Kestemont, P., Kuhn, E.R. and Foster, A. 19 Seasonal changes in plasma levels of steroid hormones in an asynchronous fish the gudgeon Gobio gobio L; (Teleosti, Cyprinidae). General and Comparative Endocrinology, 92: 168-178. Roberts, S.B., Jackson, L.F., King, W.V., Taylor, R.G., Grier, H.J. and Sullivan, C.V. 1999. Annual reproductive cycle of the common snook: endocrine correlates of maturation. Transactions American Fisheries Society, 128: 436–445.
  • Schulz, R.W. and Miura, T. 2002. Spermatogenesis and its endocrine regulation. Fish Physiology and Biochemistry, 26: 43-56.
  • Sokolowska, M., Peter, R.E., Nahomiak, C.S., Pan, C.H., Chang, J.P., Grim, L.W. and Weil, C. 1984. Induction of ovulation in goldfish, Carassius auratus, by pimozide and analogues of LHRH. Aquaculture, 36: 71Truscott, B., Idler, D.R., So, Y.P. and Walsh, J.M. 1986. Maturational steroids and gonadotropin in upstream migratory sockeye salmon. General and Comparative Endocrinology, 62: 99–110.
  • Whitehead, C., Bromage, N.R. and Breton, B. 1983. Changes in plasma levels of gonadotropins, oestradiol 17β and vitellogenin during the first and subsequent reproductive cycles of female rainbow trout. Aquaculture, 34: 317-326.

Effect of Synthetic Luteinizing Hormone - Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus frisii kutum

Year 2013, Volume: 13 Issue: 1, - , 01.02.2013

Mohaddeseh Ahmadnezhad Shahrbanoo Oryan Homayoun Hosseinzadeh Sahafi Hossein Khara

https://doi.org/10.4194/1303-2712-v13_1_12

Abstract

To assess changes of gonad development and sex steroids hormones of R. frisii kutum, broodfish were captured during spawning season 2010 (March-April) and induced intramuscularly with following hormone treatments: single injections of 1 μg kg-1 b.w. LHRH-A2, combination of LHRH-A2 with two dopamine antagonists as follows: 1 μg kg-1 b.w. LHRH-A2 + 5 mg kg-1 b.w. PIM, 1 μg kg-1 b.w. LHRH-A2 + 2.5 mg kg-1 b.w. chlorpromazine, 1 μg kg-1 b.w. LHRH-A2 + 5 mg kg-1 b.w. PIM + 2.5 mg kg-1 b.w. chlorpromazine, single injections of 5 mg kg-1 b.w. PIM and 2.5 mg kg-1 b.w. chlorpromazine, carp pitatury extract (CPE), saline solution (NaCl 0.7%) and control group (without injection) were used, respectively. The number of ovulated fish was different in each treatment. Ovaries were more progressed in ovulated rather than non-ovulated fish. Also, sex hormones such as testosterone (T) and estradiol (E2) were changed in different hormonal treatments. In ovulated fish, histological analysis revealed six stages in ovaries, while ovaries in non-ovulated ones were developed only in fourth stage

Keywords

Spawning season Histology Steroid profiles Rutilus frisii kutum

References

  • Chang, J.P. and Peter, R.E. 1983. Effects of pimozide and des Gly’O [o-Ala61 luteinizing hormone- releasing hormone ethylamide on serum gonadotropin concentrations, germinal vesicle migration and ovulation in female goldfish, Carassius auratus. General and Comparative Endocrinology, 52: 30-37.
  • Chang, J., Peter, R.E., Nahorniak, C.S. and Sokolowska, M. 1984. Effects of catecholaminergic agonists and antagonists on serum gonadotropin concentrations and ovulation in goldfish: Evidence for specificity of dopamine inhibition of gonadotropin secretion. General and Comparative Endocrinology, 55: 3513
  • Dahle, R., Taranger, G.L., Karlsen, O., Kjesbu, O.S. and Norberg, B. 2003. Gonadal development and associated changes in liver size and sexual steroids during the reproductive cycle of captive male and female Atlantic cod (Gadus morhua L.). Comparative Biochemistry and Physiology, 136: 641-653.
  • De Leeuw, R., Resink, J.W., Rooyakkers, E.J.M. and Goos, H.J. 1985a. Pimozide modulates the luteinizing hormone-releasing hormone effect on gonadotropin release in the African catfish, Clarias lazera. General and Comparative Endocrinology, 58: 120-127.
  • De Leeuw, R., Goos, H.J., Richter, C.J.J. and Eding, E.H. 1985b. Pimozide-LHRHa induced breeding of the African catfish, Clarias gariepinus (Burchell). Aquaculture, 44: 295-302.
  • Emadi, H. 1995. Rutilus frissi kutum is being victim of management problem, rapacity and tradition. J Aquatic, 3: 10-12.
  • Fostier, A., Jalabert, B., Billard, R., Breton, B. and Zohar, Y. 1983. The gonadal steroids. In: WS Hoar, DJ, Randall, Donaldson EM (Eds). Fish Physiology. (9A). Academic Press, New York.
  • Gothilf, Y., Meiri, I., Elizur, A. and Zohar, Y. 1997. Preovulatory changes in the levels of three gonadotropin-releasing hormone-encoding messenger ribonucleic acids (mRNAs), gonadotropin betasubunit mRNAs, plasma gonadotropin, and steroids in the female gilthead seabream, Sparus aurata. Biology of Reproduction, 5: 1145-54.
  • Kesteven, G.L. 1960. Manual of Field Methods in Fisheries Biology. F.A.O. Manual fish science, 1: 1-52.
  • Kobayashi, M., Aida, K. and Hanyu, U. 1987. Hormone changes during ovulation and effects of steroid hormones on plasma gonadotropin levels and ovulation in goldfish. General and Comparative Endocrinology, 67: 24-32.
  • Kozul, V., Scaramuca, B., Glamuzina, B., Glavic, N. and Tutman, P. 2001. Comparative gonadogenesis and hormonal induction of spawning of cultured and wild Mediterranean amberjack (Seriola dumerili, Risso 1810). Scientia Marina, 65: 215-220.
  • Lamba, V., Goswami, S.V. and Sundararaj, B.I. 1983. Circannual and circadian variations in plasma levels of steroids (Cortisol, estradiol-17b, estrone, and testosterone) correlated with the annual gonadal cycle in the catfish, Heteropneustes fossilis (Bloch). General and Comparative Endocrinology, 50: 2052
  • Levavi-Zermonsky, B. and Yaron, Z. 1986. Changes in gonadotropin and ovarian- steroids associated with oocytes maturation during spawning induction in the carp. General and Comparative Endocrinology, 62: 89Lokman, P.M., Vermeulen, G.J., Lambert, J.G.D. and Young, G. 1998. Gonad histology and plasma steroid profiles in wild New Zealand freshwater eels (Anguilla dieffenbachii and A. australis) before and at the onset of the natural spawning migration. Fish Physiology and Biochemistry, 19: 325–338.
  • Malison, J.A., Procarione, T.P., Barry, A, Kapuscinski, A.R. and Kayes, T.B. 1994. Endocrine and gonadal changes during the annual reproductive cycle of the freshwater teleost, Stizostedion virteum. Fish 100 Physiology and Biochemistry, 13: 473-484. Matsuyama, M.S., Adachi, Y., Nagahama, K. and Maruyama, S. 1990. Diurnal rhythm of serum steroid hormone levels in the Japanese whiting, Sillago japonica, a daily spawning teleost. Fish Physiology and Biochemistry, 8: 329-338. Matsuyama, M., Adachi, S., Nagahama, Y., Kitajima, C. and Matsuura, S. 1991. Annual reproductive cycle of the captive female Japanese sardine Sardinops melanostictus: relationship to ovarian development and serum levels of gonadal steroid hormones. Marine Biology, 108: 21-29. Nagahama, Y. 1987. Gonadotropin action on gametogenesis and steroidogenesis in Teleost gonads. Zoological Science, 4: 209-222. Nagahama, Y. 1994. Endocrine regulation of gametogenesis in fish. International Journal of Developmental Biology, 38: 217-229. Pankhurst, N.W., Stacey, N.E. and Van Der Kraak, G. 1986. Reproductive development and plasma levels of reproductive hormones of goldeye, Hiodon alosoides (Rafinesque), taken from the North Saskatchewan River during the open-water season. Canadian Journal of Zoology, 64: 2843–2849. Pankhurst, N.W. and Conroy, A.M. 1988. Endocrine changes during gonadal maturation and spawning in the orange roughy (Hoplostethus atlanticus Colett), a teleost from the midslope waters off New Zealand. General and Comparative Endocrinology, 70: 262– 2 Persson, P., Sundell, K., Bjornsson, B.T.H. and Lundovist, H. 1998. Calcium metabolism and osmoregulation during sexual Maturation of river running Atlantic salmon. Journal of Fish Biology, 52: 334-349. Peter, R.E., Crim, L.W., Goos, H.J.T. and Grim, J.W. 1978. Lesioning studies on the gravid female goldfish: Neuroendocrine regulation of ovulation. General and Comparative Endocrinology, 35: 391-401 Pham, H.Q., Nguyen, A.T., Nguyen, M.D. and Arukwe, A. 20 Sex steroid levels, oocyte maturation and spawning performance in Waigieu sea perch (Psammoperca waigiensis) exposed to thyroxin, human chorionic gonadotropin, luteinizing hormone releasing hormone and carp pituitary extract. Comparative Biochemistry and Physiology, 155: 2232
  • Planas, J.V., Athos, J., Goetz, F.W. and Swanson, P. 2000. Regulation of ovarian steroidogenesis in vitro by follicle-stimulating hormone and luteinizing hormone during sexual maturation in salmonid fish. Biology of Reproduction, 62: 1262-1269.
  • Rinchard, J., Kestemont, P., Kuhn, E.R. and Foster, A. 19 Seasonal changes in plasma levels of steroid hormones in an asynchronous fish the gudgeon Gobio gobio L; (Teleosti, Cyprinidae). General and Comparative Endocrinology, 92: 168-178. Roberts, S.B., Jackson, L.F., King, W.V., Taylor, R.G., Grier, H.J. and Sullivan, C.V. 1999. Annual reproductive cycle of the common snook: endocrine correlates of maturation. Transactions American Fisheries Society, 128: 436–445.
  • Schulz, R.W. and Miura, T. 2002. Spermatogenesis and its endocrine regulation. Fish Physiology and Biochemistry, 26: 43-56.
  • Sokolowska, M., Peter, R.E., Nahomiak, C.S., Pan, C.H., Chang, J.P., Grim, L.W. and Weil, C. 1984. Induction of ovulation in goldfish, Carassius auratus, by pimozide and analogues of LHRH. Aquaculture, 36: 71Truscott, B., Idler, D.R., So, Y.P. and Walsh, J.M. 1986. Maturational steroids and gonadotropin in upstream migratory sockeye salmon. General and Comparative Endocrinology, 62: 99–110.
  • Whitehead, C., Bromage, N.R. and Breton, B. 1983. Changes in plasma levels of gonadotropins, oestradiol 17β and vitellogenin during the first and subsequent reproductive cycles of female rainbow trout. Aquaculture, 34: 317-326.
There are 19 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Mohaddeseh Ahmadnezhad This is me

Shahrbanoo Oryan This is me

Homayoun Hosseinzadeh Sahafi This is me

Hossein Khara This is me

Publication Date February 1, 2013
Published in Issue Year 2013 Volume: 13 Issue: 1

Cite

APA Ahmadnezhad, M., Oryan, S., Sahafi, H. H., Khara, H. (2013). Effect of Synthetic Luteinizing Hormone - Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus frisii kutum. Turkish Journal of Fisheries and Aquatic Sciences, 13(1). https://doi.org/10.4194/1303-2712-v13_1_12
AMA Ahmadnezhad M, Oryan S, Sahafi HH, Khara H. Effect of Synthetic Luteinizing Hormone - Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus frisii kutum. Turkish Journal of Fisheries and Aquatic Sciences. February 2013;13(1). doi:10.4194/1303-2712-v13_1_12
Chicago Ahmadnezhad, Mohaddeseh, Shahrbanoo Oryan, Homayoun Hosseinzadeh Sahafi, and Hossein Khara. “Effect of Synthetic Luteinizing Hormone - Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus Frisii Kutum”. Turkish Journal of Fisheries and Aquatic Sciences 13, no. 1 (February 2013). https://doi.org/10.4194/1303-2712-v13_1_12.
EndNote Ahmadnezhad M, Oryan S, Sahafi HH, Khara H (February 1, 2013) Effect of Synthetic Luteinizing Hormone - Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus frisii kutum. Turkish Journal of Fisheries and Aquatic Sciences 13 1
IEEE M. Ahmadnezhad, S. Oryan, H. H. Sahafi, and H. Khara, “Effect of Synthetic Luteinizing Hormone - Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus frisii kutum”, Turkish Journal of Fisheries and Aquatic Sciences, vol. 13, no. 1, 2013, doi: 10.4194/1303-2712-v13_1_12.
ISNAD Ahmadnezhad, Mohaddeseh et al. “Effect of Synthetic Luteinizing Hormone - Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus Frisii Kutum”. Turkish Journal of Fisheries and Aquatic Sciences 13/1 (February 2013). https://doi.org/10.4194/1303-2712-v13_1_12.
JAMA Ahmadnezhad M, Oryan S, Sahafi HH, Khara H. Effect of Synthetic Luteinizing Hormone - Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus frisii kutum. Turkish Journal of Fisheries and Aquatic Sciences. 2013;13. doi:10.4194/1303-2712-v13_1_12.
MLA Ahmadnezhad, Mohaddeseh et al. “Effect of Synthetic Luteinizing Hormone - Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus Frisii Kutum”. Turkish Journal of Fisheries and Aquatic Sciences, vol. 13, no. 1, 2013, doi:10.4194/1303-2712-v13_1_12.
Vancouver Ahmadnezhad M, Oryan S, Sahafi HH, Khara H. Effect of Synthetic Luteinizing Hormone - Releasing Hormone (LHRH-A2) Plus Pimozide and Chlorpromazine on Ovarian Development and Levels of Gonad Steroid Hormones in Female Kutum Rutilus frisii kutum. Turkish Journal of Fisheries and Aquatic Sciences. 2013;13(1).